CHANGES IN THE DISTRIBUTION OF A HISTONE IN DORSAL ROOT GANGLION NEURONS DURING DEVELOPMENT

The isolation and cellular localization of a basic protein (histone) from central nervous tissue have been previously reported. In the tissues previously studied (nervous tissue, testis, liver, spleen, kidney, ovary), the basic protein was restricted in distribution to the nuclei of neurons and spermatogonia. In the present study, the temporal appearance of the histone within neurons and the changes in its distribution during ontogenesis were examined. The reaction between a fluorescent immune γ-globulin prepared against this purified tissue-specific histone and the neurons from the dorsal root ganglia of the rat was investigated. The dorsal root ganglia examined were those from fetuses, 2-, 10-, and 40-day-old rats, and from adult rats. At the earliest stages, only the nucleoli reacted. Subsequently, threads of fluorescent material were seen to emerge from the nucleoli. The extent of this reaction between the immune globulin and the threads within the nuclei continued to increase with maturation. No changes in fluorescence localization during development could be seen in the nuclei of neurons in the cerebellum or brain stem. The role that this tissue-specific histone may play in cell function is discussed.

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Generation of New Neurons in Dorsal Root Ganglia in Adult Rats after Peripheral Nerve Crush Injury

Neural Plasticity ◽

10.1155/2015/860546 ◽

2015 ◽

Vol 2015 ◽

pp. 1-12 ◽

Cited By ~ 11

Author(s):

Luisa Muratori ◽

Giulia Ronchi ◽

Stefania Raimondo ◽

Stefano Geuna ◽

Maria Giuseppina Giacobini-Robecchi ◽

...

Keyword(s):

Sensory Neurons ◽

Dorsal Root Ganglia ◽

Dorsal Root ◽

Morphological Changes ◽

Dorsal Root Ganglion Neurons ◽

Cell Phenotype ◽

Adult Rats ◽

Nerve Crush ◽

Satellite Glial Cells ◽

Ganglion Neurons

The evidence of neurons generatedex novoin sensory ganglia of adult animals is still debated. In the present study, we investigated, using high resolution light microscopy and stereological analysis, the changes in the number of neurons in dorsal root ganglia after 30 days from a crush lesion of the rat brachial plexus terminal branches. Results showed, as expected, a relevant hypertrophy of dorsal root ganglion neurons. In addition, we reported, for the first time in the literature, that neuronal hypertrophy was accompanied by massive neuronal hyperplasia leading to a 42% increase of the number of primary sensory neurons. Moreover, ultrastructural analyses on sensory neurons showed that there was not a relevant neuronal loss as a consequence of the nerve injury. The evidence of BrdU-immunopositive neurons and neural progenitors labeled with Ki67, nanog, nestin, and sox-2 confirmed the stereological evidence of posttraumatic neurogenesis in dorsal root ganglia. Analysis of morphological changes following axonal damage in addition to immunofluorescence characterization of cell phenotype suggested that the neuronal precursors which give rise to the newly generated neurons could be represented by satellite glial cells that actively proliferate after the lesion and are able to differentiate toward the neuronal lineage.

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Distribution patterns of different P2x receptor phenotypes in acutely dissociated dorsal root ganglion neurons of adult rats

Experimental Brain Research ◽

10.1007/s002210000414 ◽

2000 ◽

Vol 134 (1) ◽

pp. 126-132 ◽

Cited By ~ 41

Author(s):

J.C. Petruska ◽

B.Y. Cooper ◽

R.D. Johnson ◽

J.G. Gu

Keyword(s):

Dorsal Root Ganglion ◽

Dorsal Root ◽

Distribution Patterns ◽

Dorsal Root Ganglion Neurons ◽

P2x Receptor ◽

Adult Rats ◽

Ganglion Neurons

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6. Dorsal root ganglion neurons: electrical properties and cell type

Philosophical Transactions of the Royal Society of London Series B Biological Sciences ◽

10.1098/rstb.1985.0046 ◽

1985 ◽

Vol 308 (1136) ◽

pp. 412-413

Keyword(s):

Electrical Properties ◽

Dorsal Root Ganglion ◽

Dorsal Root Ganglia ◽

Dorsal Root ◽

Dorsal Root Ganglion Neurons ◽

Cell Type ◽

Neuronal Types ◽

Ganglion Neurons ◽

Two Populations ◽

Normally Distributed

It has been shown that there are two main morphological neuronal types in rat and mouse dorsal root ganglia (d.r.g.s), the large light (LL) and small dark (s.d.) neurons. Each population has a normally distributed range of cell sizes with different means, and the size ranges of the two populations overlap (Lawson 1979; Lawson & Harper, in press).

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