scholarly journals EXTRACTION, HYDROLYSIS, AND ELECTROPHORETIC ANALYSIS OF RIBONUCLEIC ACID FROM MICROSCOPIC TISSUE UNITS (MICROPHORESIS)

1960 ◽  
Vol 8 (1) ◽  
pp. 39-46 ◽  
Author(s):  
Jan-Erik Edström
Keyword(s):  
Quantitative Determination ◽  
Ultraviolet Light ◽  
Coefficient Of Variation ◽  
Ribonucleic Acid ◽  
Single Cells ◽  
Electrophoretic Analysis ◽  
Test Substance ◽  
Different Sources ◽  
Purine Pyrimidine

A procedure for the purine-pyrimidine analysis of RNA in the 100- to 1000 µµg. range is presented. It includes hydrolysis and electrophoretic analysis of RNA, which is extracted from single isolated tissue units, like single cells. The quantitative determination of the separated compounds is carried out by a photographic-photometric procedure in ultraviolet light. The determined values show a coefficient of variation of about ±7 per cent on test substance. Microelectrophoretic analyses of RNA from different sources have been performed and are compared to macrochemical analyses. The agreement is good in those cases in which it is possible to get any information at all through macrochemical analyses.

Rapid Method for Quantitative Determination of Propoxyphene in Serum by Gas-Liquid Chromatography

1973 ◽  
Vol 19 (5) ◽  
pp. 492-495 ◽  
Author(s):  
M A Evenson ◽  
Susan Koellner
Keyword(s):  
Liquid Chromatography ◽  
Quantitative Determination ◽  
Rapid Method ◽  
Coefficient Of Variation ◽  
Therapeutic Dose ◽  
Gas Liquid Chromatography ◽  
Healthy Controls ◽  
Serum Pool ◽  
Sedative Drugs

Abstract Rapid, accurate, and precise gas-chromatographic methods are reported for measurement of propoxyphene ("Darvon") in serum. A sample of 5 ml of serum is required for quantitation in blood after a therapeutic dose of 130-195 mg of propoxyphene; in cases of overdose of propoxyphene, only 1 ml of serum is required in a "toxic method" variation. Neither serum from healthy controls or from hospitalized patients contains interfering substances. Several commonly used analgesic and sedative drugs, added to a serum pool, also did not interfere. Day-to-day precision of the therapeutic method, as measured by the coefficient of variation (CV), is 7%; the CV for the method as applied to overdose cases is less than 3%. Propoxyphene added to serum could be about 86% accounted for analytically.

The Quantitative Determination of Mixed Sulfonamides by Paper Chromatography

1963 ◽  
Vol 46 (5) ◽  
pp. 899-901
Author(s):  
Frieda M Kunze ◽  
Luis Espinoza B
Keyword(s):  
Quantitative Determination ◽  
Ultraviolet Light ◽  
Paper Chromatography ◽  
Short Wavelength ◽  
Mean Deviation ◽  
Marshall Technique ◽  
Quantitative Procedure

Abstract A simplified quantitative procedure for the determination of mixed sulfonamides in pharmaceuticals is presented. The components are resolved by paper chromatography and located by their fluorescence under short wavelength ultraviolet light. They are then eluted and determined colorimetrically by a revised Bratton-Marshall technique. Total time of analysis is less than 4 hours. Recoveries averaged 98.8% with a range of 95.7 to 103.4%. Mean deviation from the average was 1.4%.

Methods For The Quantitative Determination Of Biocides In Tobacco And Tobacco Products. Part V: A Method For Rapid Determination Of The Urea Herbicide Metobromuron/Methoden Zur Quantitativen Bestimmung Von Bioziden In Tabak Und Tabakerzeugnissen. V. Mitteilung: Eine Schnellmethode Zur Bestimmung Des Harnstoff-Herbizides Metobromuron (Patoran)

1974 ◽  
Vol 7 (5) ◽  
Author(s):  
E. Nesemann ◽  
F. Seehofer
Keyword(s):  
Quantitative Determination ◽  
Coefficient Of Variation ◽  
Chromatographic Separation ◽  
Rapid Determination ◽  
Tobacco Products ◽  
Aqueous Acetonitrile ◽  
Gas Chromatographic Separation

AbstractDuring the attempt to transfer to tobacco a method commonly used to determine metobromuron in foodstuffs, it became apparent that only very small amounts of metobromuron and less than 0.1 ppm 4-bromoanilin were found. Nor did the modification of this method produce better results, because metobromuron, apparently linked to tobacco components, cannot be extracted with aqueous acetonitrile. The tobacco was then distilled under hydrolysing conditions and the 4-bromoanilin released was bound as hydrochloride. After gas-chromatographic separation 4-bromoanilin is determined specifically with the microcoulometer (bromine) and with the Coulson detector (nitrogen). The amount of metobromuron is calculated from the 4-bromoanilin. With this method no difference can be made between intact metobromuron and 4-bromoanilin already metabolised from metobromuron. Recovery is 90 % for 4-bromoanilin and 92 % for metobromuron, the coefficient of variation is 3.0 %. In cigarettes whose tobacco had grown in soil which had been treated with 4 kg of Patoran per hectare before transplanting, a mean of 5.8 ppm metobromuron was found by this procedure.

Methods for the Quantitative Determination of Biocides in Tobacco and Tobacco Products. Part IV: A Method for the Determination of Maleic Hydrazide in Tobacco/Methoden zur quantitativen Bestimmung von Bioziden in Tabak und Tabakerzeugnissen. IV. Mitteilung: Eine Methode zur Bestimmung von Maleinsäurehydrazid

1974 ◽  
Vol 7 (4) ◽  
Author(s):  
E. Nesemann ◽  
H. Rabitz ◽  
F. Seehofer
Keyword(s):  
Activated Carbon ◽  
Quantitative Determination ◽  
Coefficient Of Variation ◽  
Maleic Hydrazide ◽  
Routine Analysis ◽  
Purification Step ◽  
Tobacco Products

AbstractThe author's method (developed in 1966) for the determination of maleic hydrazide in tobacco has been improved by the introduction of a purification step using activated carbon. Interferences produced by reactive compounds other than maleic hydrazide can thus be eliminated. The procedure proved to be suitable for routine analysis. The coefficient of variation was found to range from 1 to 7 %. Added amounts of maleic hydrazide have been recovered at the 90-105 % level. The accuracy of the procedure could be confirmed by results comparatively obtained in three laboratories

Perchlorate in Sea Water

1961 ◽  
Vol 41 (1) ◽  
pp. 175-186 ◽  
Author(s):  
R. Greenhalgh ◽  
J. P. Riley
Keyword(s):  
Aqueous Solution ◽  
Quantitative Determination ◽  
Water Sample ◽  
Coefficient Of Variation ◽  
Sea Water ◽  
Absorption Spectrophotometry ◽  
Infra Red ◽  
Tetraphenylarsonium Chloride

The use of tetraphenylarsonium chloride for the detection and determination of perchlorate in sea water has been investigated. An aqueous solution of this reagent gives a visible precipitate of tetraphenylarsonium perchlorate with sea water containing 5 μg ClO4-/ml. within 10 min. For the quantitative determination of traces of perchlorate, potassium perrhenate is added to the water sample, and the tetraphenylarsonium perrhenate, which is precipitated by addition of the reagent, co-precipitates tetraphenylarsonium perchlorate. Perchlorate is determined in the mixed precipitate by infra-red absorption spectrophotometry. Using 100 ml. of sea water, a sensitivity of 0·03 μg ClO4-/ml. can be attained, with a coefficient of variation of ca. 10%. No interference is caused by the ions normally present in sea water.

Spectrophotometric Determination of Ethchlorvynol in Biologic Specimens

1974 ◽  
Vol 20 (2) ◽  
pp. 159-162 ◽  
Author(s):  
Jack E Wallace ◽  
Horace E Hamilton ◽  
Joel A Riloff ◽  
Kenneth Blum
Keyword(s):  
Quantitative Determination ◽  
Ultraviolet Light ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Product Mixture

Abstract A sensitive, highly specific spectrophotometric method for quantitative determination of ethchlorvynol in biologic specimens is described. The procedure is based on conversion of ethchlorvynol to a product mixture consisting of two major components that strongly absorb ultraviolet light. Results can be obtained within 45 min of receipt of a specimen.

Rapid Gas-Liquid Chromatographic Method for Determination of Sulfamethazine in Swine Feed

1982 ◽  
Vol 65 (5) ◽  
pp. 1048-1053
Author(s):  
Robert K Munns ◽  
Jose E Roybal
Keyword(s):  
Quantitative Determination ◽  
Coefficient Of Variation ◽  
Chromatographic Method ◽  
Internal Standard ◽  
Liquid Chromatographic Method ◽  
Average Coefficient ◽  
Swine Feed ◽  
Liquid Chromatographic

Abstract A gas-liquid chromatographic method is described for the quantitative determination of trace amounts of sulfamethazine in swine feed. Sulfamethazine is extracted in ammoniated acetone and isolated from other extractants on a Sephadex LH-20 column. The eluate is methylated with diazomethane and evaporated to dryness. The residue is dissolved in a solvent containing an internal standard of methyl sulfasymazine before being injected onto an OV-25 GLC column. An estimation of precision was established by assaying 10 sets of swine feed fortified with 0.5, 1,2, and 5 ppm SMZ. Mean recoveries were 96.0,94.3, 93.5, and 94.0%, respectively, with an average coefficient of variation of 3.07%. The critical steps and ruggedness of the method were also determined.

Sign in / Sign up

Export Citation Format

Share Document