Intracellular transport of the transmembrane glycoprotein G of vesicular stomatitis virus through the Golgi apparatus as visualized by electron microscope radioautography.
Keyword(s):
The intracellular migration of G protein in vesicular stomatitis virus-infected cells was visualized by light and electron microscope radioautography after a 2-min pulse with [3H]mannose followed by nonradioactive chase for various intervals. The radioactivity initially (at 5-10 min) appeared predominantly in the endoplasmic reticulum, and the [3H]mannose-labeled G protein produced was sensitive to endoglycosidase H. Silver grains were subsequently (at 30-40 min) observed over the Golgi apparatus, and the [3H]mannose-labeled G protein became resistant to endoglycosidase H digestion. Our data directly demonstrate the intracellular transport of a plasmalemma-destined transmembrane glycoprotein through the Golgi apparatus.
1990 ◽
Vol 110
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pp. 625-635
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1980 ◽
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pp. 162-171
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1979 ◽
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pp. 416-426
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1983 ◽
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pp. 1815-1822
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1985 ◽
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pp. 3074-3083
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1985 ◽
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pp. 2036-2046
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1994 ◽
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pp. 99-104
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Keyword(s):
1984 ◽
Vol 99
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pp. 260-271
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