scholarly journals VISUALIZATION OF EGYPT 101 VIRUS IN THE MOUSE'S BRAIN AND IN CULTURED HUMAN CARCINOMA CELLS BY MEANS OF FLUORESCENT ANTIBODY

1955 ◽  
Vol 102 (3) ◽  
pp. 243-248 ◽  
Author(s):  
Wilbur Fiske Noyes
Keyword(s):  
Cultured Cells ◽  
Fluorescent Antibody ◽  
Carcinoma Cells ◽  
Fluorescent Antibody Technique ◽  
Human Carcinoma ◽  
Antibody Technique ◽  
Human Cell Cultures ◽  
Brain And Spinal Cord ◽  
The Brain ◽  
Human Epidermoid Carcinoma

The antigens of the Egypt virus have been detected by means of the fluorescent antibody technique in the neurons of the brain and spinal cord, in both sensory and motor areas, of infected mice. The antigens have also been observed in infected human epidermoid carcinoma cells in culture. In both cases the antigens occurred in the cytoplasm of the cells exclusively. In the early stages of infection the antigen was localized about the nucleus of the human cultured cells. In some cases the staining of the antigens produced a sponge-like effect, probably because of the numerous vacuoles of lipid material. The antigens were observed in the protoplasmic connections between cells and they probably served as a main route of spread of the agent in these human cell cultures. The number of cells initially infected and showing antigen was proportional to the amount of virus added, and titration with one substrain of cells proved much more sensitive than intracerebral titration in the mouse.

Accelerated detection of lymphocytic choriomeningitis virus in diagnostic specimens

1976 ◽  
Vol 22 (11) ◽  
pp. 1662-1663
Author(s):  
Vester J. Lewis ◽  
W. Lanier Thacker ◽  
Shannon H. Mitchell
Keyword(s):  
Fluorescent Antibody ◽  
Lymphocytic Choriomeningitis ◽  
Lymphocytic Choriomeningitis Virus ◽  
Fluorescent Antibody Technique ◽  
Optimal Route ◽  
Antibody Technique ◽  
Clinical Specimens ◽  
The Brain

Lymphocytic choriomeningitis virus could be demonstrated earlier in mice inoculated with clinical specimens if the mice were sacrificed before they appeared sick for examination by the fluorescent antibody technique. In the procedure, the optimal route for inoculation was intracerebral and the best organ tested for staining was the brain.

scholarly journals An abortion storm in dairy cattle associated with neosporosis in southern Brazil

2021 ◽  
Vol 30 (2) ◽  
Author(s):  
João Henrique Perotta ◽  
Bárbara Barbi de Freitas ◽  
Nicoly Nayana Marcom ◽  
Caroline Argenta Pescador ◽  
Cláudia Carnielli Pereira ◽  
...  
Keyword(s):  
Neospora Caninum ◽  
Fluorescent Antibody ◽  
Dairy Herd ◽  
Eastern Region ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Southern Brazil ◽  
Antibody Technique ◽  
Blood Samples ◽  
The Brain

Abstract Between December 2016 and April 2017, a spate of abortions occurred in a closed dairy herd from the central eastern region of Paraná, Brazil, in which 75 cows aborted. To identify its cause, organ fragments were collected from an aborted fetus for histopathology, and the blood samples from a stillborn, 4 aborted fetuses, and 9 farm dogs for indirect fluorescent antibody technique (IFAT). These tests found multifocal non-suppurative encephalitis, periportal hepatitis, and multifocal lymphoplasmacytic myocarditis, and detected anti-Neospora antibodies in all aborted fetuses, and in 5 of the 9 dogs. DNA of Neospora caninum was detected in the brain tissue of an aborted fetus. Blood samples of 340 cows and 146 heifers showed 33.5% and 30.8% seropositivity, respectively. In this closed herd, the parasite was probably introduced by infected domesticated or wild carnivores inhabiting the farm, through the infective oocysts present in their stool.

scholarly journals Comparison of purified anti-actin and fluorescent-heavy meromyosin staining patterns in dividing cells.

1979 ◽  
Vol 80 (3) ◽  
pp. 509-520 ◽  
Author(s):  
I M Herman ◽  
T D Pollard
Keyword(s):  
Cultured Cells ◽  
Direct Method ◽  
Fluorescent Antibody ◽  
Stress Fibers ◽  
Fluorescent Antibody Technique ◽  
Heavy Meromyosin ◽  
Antibody Technique ◽  
Nonmuscle Cells ◽  
Dividing Cells ◽  
Fluorescent Heavy Meromyosin

We purified actin antibodies by affinity chromatography from the serum of rabbits immunized with glutaraldehyde-fixed chicken gizzard actin filaments and used this anti-actin to localize actin in myofibrils and fixed cultured cells at each stage of the cell cycle. By double immunodiffusion the anti-actin reacted with both smooth and skeletal muscle actin. Several blocking and absorption experiments demonstrated that the antibodies also bound specifically to actin in nonmuscle cells. The same structures stained using either the direct or the indirect fluorescent antibody technique; and, while the indirect method was more sensitive, the direct method was superior because there was no detectable nonspecific staining. As expected, anti-actin stained the I-band of myofibrils. It also stained stress fibers and membrane ruffles in HeLa cells. Some PtK-2 cells have straight stress fibers which stained with anti-actin, but in confluent cultures all PtK-2 cells have, instead, sinuous phase-dense fibers which stained with antibody. At prophase the whole cytoplasm stained uniformly with anti-actin. During metaphase and anaphase, anti-actin staining was concentrated diffusely in the mitotic spindle. In contrast, fluorescent heavy meromyosin stained discrete fine spindle fibers in these fixed cells. During cytokinesis, anti-actin stained the whole cytoplasm uniformly and was not concentrated in the cleavage furrow.

Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method

2000 ◽  
Vol 41 (4-5) ◽  
pp. 301-308 ◽  
Author(s):  
N. Noda ◽  
H. Ikuta ◽  
Y. Ebie ◽  
A. Hirata ◽  
S. Tsuneda ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Fluorescent Antibody ◽  
Nitrifying Bacteria ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Antibody Method ◽  
In Situ Detection ◽  
Rapid Quantification

Fluorescent antibody technique by the monoclonal antibody method is very useful and helpful for the rapid quantification and in situ detection of the specific bacteria like nitrifiers in a mixed baxterial habitat such as a biofilm. In this study, twelve monoclonal antibodies against Nitrosomonas europaea (IFO14298) and sixteen against Nitrobacter winogradskyi (IFO14297) were raised from splenocytes of mice (BALB/c). It was found that these antibodies exhibited little cross reactivity against various kinds of heterotrophic bacteria. The direct cell count method using monoclonal antibodies could exactly detect and rapidly quantify N. europaea and N. winogradskyi. Moreover, the distribution of N. europaea and N. winogradskyi in a biofilm could be examined by in situ fluorescent antibody technique. It was shown that most of N. winogradskyi existed near the surface part and most of N. europaea existed at the inner part of the polyethylene glycol (PEG) gel pellet, which had entrapped activated sludge and used in a landfill leachate treatment reactor. It was suggested that this monoclonal antibody method was utilized for estimating and controlling the population of nitrifying bacteria as a quick and favorable tool.

Rubella Antibodies in Human Serum: Detection by the Indirect Fluorescent-Antibody Technique

Science ◽  
1964 ◽  
Vol 145 (3635) ◽  
pp. 943-945 ◽  
Author(s):  
G. C. Brown ◽  
H. F. Maassab ◽  
J. A. Veronelli ◽  
T. J. Francis
Keyword(s):  
Human Serum ◽  
Fluorescent Antibody ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Antibody Technique ◽  
Serum Detection

scholarly journals LOCALIZATION OF UROMUCOID IN HUMAN KIDNEY AND IN SECTIONS OF HUMAN KIDNEY STONE WITH THE FLUORESCENT ANTIBODY TECHNIQUE

1965 ◽  
Vol 13 (3) ◽  
pp. 155-160 ◽  
Author(s):  
H. J. KEUTEL
Keyword(s):  
Kidney Stones ◽  
Kidney Stone ◽  
Fluorescent Antibody ◽  
Human Kidney ◽  
Fluorescent Antibody Technique ◽  
Renal Tubules ◽  
Antibody Technique ◽  
The Matrix ◽  
Normal Human ◽  
The Common

Fluorescent labeled antibodies were used for the demonstration of uromucoid. This urine specific mucoprotein is demonstrably present only in the epithelial cells of the proximal segments of the normal human renal tubules and in the matrix of human kidney stones of all the common crystalline compositions.

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