scholarly journals SPECIFIC IMMUNE RESPONSE GENES OF THE GUINEA PIG

1971 ◽  
Vol 134 (6) ◽  
pp. 1538-1544 ◽  
Author(s):  
Harry G. Bluestein ◽  
Ira Green ◽  
Baruj Benacerraf
Keyword(s):  
Immune Response ◽  
Lymph Node ◽  
Guinea Pig ◽  
Guinea Pigs ◽  
Genetic Locus ◽  
Cytolytic Activity ◽  
Immune Response Gene ◽  
Response Gene ◽  
Large Measure ◽  
Lymph Node Cells

The lymph node cells from all L-glutamic acid and L-tyrosine (GT) responder random-bred guinea pigs were susceptible to lysis by strain 2 anti-strain 13 isoantisera in the presence of complement. These same antisera were cytolytic for lymph node cells of only some of the GT nonresponder animals. However, after absorption with cells, from a nonresponder guinea pig, susceptible to lysis, the anti-strain 13 antisera were no longer able to lyse cells from any GT nonresponder guinea pigs while retaining a large measure of their cytolytic activity for cells of all GT responder guinea pigs. Thus, at least two major strain 13 histocompatibility specificities are expressed on the cells of random-bred guinea pigs. The genetic locus controlling the expression of only one of those strain 13 histocompatibility specificities is linked to the GT immune response gene.

scholarly journals OCCURRENCE OF DELAYED HYPERSENSITIVITY DURING THE DEVELOPMENT OF ARTHUS TYPE HYPERSENSITIVITY

1958 ◽  
Vol 107 (1) ◽  
pp. 109-124 ◽  
Author(s):  
S. B. Salvin ◽  
Keyword(s):  
Lymph Node ◽  
Guinea Pig ◽  
Latent Period ◽  
Guinea Pigs ◽  
Specific Antigen ◽  
Egg Albumin ◽  
Lymph Node Cells ◽  
Type Inclusion ◽  
Antibody Determination ◽  
Circulating Antibody

Guinea pigs were injected in the footpads with either purified diphtheria toxoid or recrystallized egg albumin in Freund adjuvant without mycobacteria. Each guinea pig was then skin-tested only once with the specific antigen and bled for antibody determination. After injection of the sensitizing antigen, a latent period occurred during which neither sensitivity nor circulating antibody could be detected. A period of delayed sensitivity followed wherein circulating antibody could not be discerned and which could be transferred by lymph node cells. Ultimately, the Arthus type sensitivity developed, accompanied by circulating antibody. The duration and severity of reactions to homologous antigens during the last 2 phases varied with the antigen and with the dose. An increase in the sensitizing dose decreased the duration of the delayed type of allergy, a decrease in the dose prolonged the delayed type. Inclusion of mycobacterium in the sensitizing inoculum tended to introduce delayed sensitivity earlier and delay the onset of Arthus type sensitivity. When specific precipitate in antibody excess was included with the toxoid in the sensitizing dose, the onset of the Arthus phase was hastened. When lymph nodes from a large number of sensitized donors were removed during the latter part of the latent period, recipients of the cells showed a delayed type sensitivity.

scholarly journals HISTOCOMPATIBILITY TYPE AND IMMUNE RESPONSIVENESS IN RANDOM BRED HARTLEY STRAIN GUINEA PIGS

1970 ◽  
Vol 132 (6) ◽  
pp. 1259-1266 ◽  
Author(s):  
William John Martin ◽  
Leonard Ellman ◽  
Ira Green ◽  
Baruj Benacerraf
Keyword(s):  
Immune Response ◽  
Inbred Strain ◽  
Guinea Pigs ◽  
Histocompatibility Antigen ◽  
Specific Immune Response ◽  
Immune Response Gene ◽  
Immune Responsiveness ◽  
Major Histocompatibility ◽  
Response Gene ◽  
Major Histocompatibility Antigen

Outbred Hartley strain guinea pigs capable of responding immunologically to 2,4-dinitrophenylated poly-L-lysine were shown to display a histocompatibility specificity in common with inbred strain 2 guinea pigs. This histocompatibility specificity was not detected in guinea pigs unable to respond immunologically to DNP-PLL. The result suggests that the poly-L-lysine specific immune response gene is very closely linked or even identical with a gene determining a major histocompatibility antigen in guinea pigs.

scholarly journals Antigen-presenting cells from nonresponder strain 2 guinea pigs are fully competent to present bovine insulin B chain to responder strain 13 T cells. Evidence against a determinant selection model and in favor of a clonal deletion model of immune response gene function.

1983 ◽  
Vol 157 (4) ◽  
pp. 1287-1299 ◽  
Author(s):  
G A Dos Reis ◽  
E M Shevach
Keyword(s):  
Immune Response ◽  
T Cells ◽  
Gene Function ◽  
Guinea Pigs ◽  
Bovine Insulin ◽  
Immune Response Gene ◽  
Cell Repertoire ◽  
Clonal Deletion ◽  
Response Gene ◽  
Selection Hypothesis

To test directly the determinant selection hypothesis of immune response gene function, we primed strain 13 T lymphocytes in vitro with allogeneic bovine insulin pulsed strain 2 macrophages. Strain 2 macrophages were found to be fully competent to present bovine insulin B chain to strain 13 T cells despite the fact that strain 2 guinea pigs are normally totally unresponsive to this antigen. These results are incompatible with a strict interpretation of the determinant selection hypothesis, which would have predicted that strain 2 macrophages would have been restricted to the presentation of A chain loop determinants. In addition, a comparison of the reactivity profiles of self-Ia- and allo-Ia-restricted strain 13 T cells to a series of synthetic B chain peptide fragments revealed that the allo-Ia-restricted populations could be activated by autologous guinea pig insulin. Taken together, these observations strongly suggest that the clonal deletion of self-reactive cells is likely to be I region restricted and that nonresponsiveness to any protein antigen may result from a restriction in the T cell repertoire that is generated during ontogeny by a clonal deletion mechanism of tolerance to self.

scholarly journals SPECIFIC IMMUNE RESPONSE GENES OF THE GUINEA PIG

1972 ◽  
Vol 135 (1) ◽  
pp. 98-109 ◽  
Author(s):  
Harry G. Bluestein ◽  
Ira Green ◽  
Paul H. Maurer ◽  
Baruj Benacerraf
Keyword(s):  
Immune Response ◽  
Immune Responses ◽  
Glutamic Acid ◽  
Guinea Pigs ◽  
Serum Antibody ◽  
Specific Immune Response ◽  
Immune Response Gene ◽  
Antigenic Determinants ◽  
Antibody Activity ◽  
Response Gene

The ability of guinea pigs to make immune responses to the random linear copolymer of L-glutamic acid and L-alanine, GA, and to L-glutamic acid and L-tyrosine, GT, is each controlled by a different immune response gene. On the other hand, the random linear terpolymer of L-glutamic acid, L-alanine, and L-tyrosine, GAT, which contains both GA and GT antigenic determinants, is immunogenic in all guinea pigs. After GAT immunization, all animals develop delayed hvpersensitivity and serum antibody specific for GAT. However, only those guinea pigs possessing the GA immune response gene demonstrate cross-reactive delayed hypersensitivity when challenged with GA. In addition, the anti-GAT antisera produced by those animals having the GA gene contain cross-reacting anti-GA antibodies. The sera from guinea pigs lacking the GA gene have no anti-GA antibody activity. Thus, we have demonstrated that a specific immune response gene controlling responsiveness to a "simple" antigen can determine the specificity of both cellular and humoral immune responses to a more complex antigen.

scholarly journals Guinea pig immune response-related histocompatibility antigens. Partial characterization and distribution.

1975 ◽  
Vol 141 (1) ◽  
pp. 27-41 ◽  
Author(s):  
F D Findelman ◽  
E M Shevach ◽  
E S Vitetta ◽  
I Green ◽  
W E Paul
Keyword(s):  
Immune Response ◽  
Lymph Node ◽  
Cell Membrane ◽  
Guinea Pig ◽  
B Cell ◽  
Polyacrylamide Gels ◽  
Histocompatibility Antigens ◽  
Lymph Node Cells ◽  
Membrane Antigens ◽  
Cell Membrane Antigens

We have previously demonstrated that guinea pig alloantisera directed at strain 2 and strain 13 membrane antigens block specific lymphocyte activation in immune response gene-controlled systems. In this communication we describe the partial characterization of the antigens against which these antisera are directed (the 2 and 13 antigens) and, in addition, that of the B antigen which by distribution resembles the human HL-A and mouse H-2 major histocompatibility antigens. Lymphoid cells from strain 2 and strain 13 guinea pigs were surface labeled with 125I by the lactoperoxidase technique. Nonidet P-40 extracts of these labeled cells were precipitated by sandwiches of strain 2 antistrain 13, strain 13 antistrain 2, or outbred anti-B antisera, followed by rabbit antiguinea pig immunoglobulin antisera. Precipitates were dissolved in sodium dodecyl sulfate (SDS) and electrophoresed on SDS polyacrylamide gels. Radioactive peaks representing the 2 and B-cell membrane antigens were obtained from strain 2 lymph node cells, as well as from a B-lymphoid cell population (L2C leukemia cells) and a T-lymphocyte population (STRAIN 2 PERITONEAL EXUDATE LYMPHOCYTES [PELs]). Radioactive peaks representing the 13 and B-cell membrane antigens were obtained from strain 13 lymph node cells and strain 13 PELs. All anti-B precipitates produced two peaks when electrophoresed on SDS polyacrylamide gels; one representing an antigen with a mol wt of approximately 45,000, and one representing an antigen with a mol wt of about 12,000. Both may be components of a single protein. All anti-2 and anti-13 precipitates produced a single peak when electrophoresed on SDS polyacrylamide gels. Both the 2 and 13 antigens were found by this technique to have mol wt of approximately 25,000. By molecular weight criteria, as well as by previously investigated distributional criteria, the B antigen is similar to the human LA and Four antigens, and to the mouse D and K antigens, and the 2 and 13 antigens are similar to the mouse Ia antigens.

scholarly journals Transfer of experimental allergic encephalomyelitis in Lewis rats using supernates of incubated sensitized lymph node cells.

1977 ◽  
Vol 145 (5) ◽  
pp. 1405-1410 ◽  
Author(s):  
C C Whitacre ◽  
P Y Paterson
Keyword(s):  
Spinal Cord ◽  
Lymph Node ◽  
Guinea Pig ◽  
Experimental Allergic Encephalomyelitis ◽  
Nervous Tissue ◽  
Allergic Encephalomyelitis ◽  
Lewis Rats ◽  
Transfer Activity ◽  
Lymph Node Cells ◽  
Experimental Allergic

Supernates derived from incubated lymph node cells of Lewis rats sensitized to guinea pig spinal cord-Freund's adjuvant transfer experimental allergic encephalomyelitis (EAE) to syngeneic recipients. EAE supernatant transfer activity (EAE-STA) is not demonstrable in supernates derived from LNC of control donors not sensitized to nervous tissue. After addition of brain antigen to active supernates, EAE-STA is not longer demonstrable.

Lack of immune response gene control for induction of epitope-specific suppression by TGAL antigen

Nature ◽  
1982 ◽  
Vol 295 (5847) ◽  
pp. 329-331 ◽  
Author(s):  
Leonore A. Herzenberg ◽  
Takeshi Tokuhisa ◽  
Kyoko Hayakawa ◽  
Leonard A. Herzenberg
Keyword(s):  
Immune Response ◽  
Immune Response Gene ◽  
Gene Control ◽  
Response Gene ◽  
Specific Suppression

scholarly journals Passive transfer of experimental autoimmune myasthenia by lymph node cells in inbred guinea pigs.

1975 ◽  
Vol 142 (3) ◽  
pp. 785-789 ◽  
Author(s):  
R Tarrab-Hazdi ◽  
A Aharonov ◽  
O Abramsky ◽  
I Yaar ◽  
S Fuchs
Keyword(s):  
Lymph Node ◽  
Human Disease ◽  
Guinea Pigs ◽  
Cellular Response ◽  
Clinical Signs ◽  
Clinical Manifestations ◽  
Passive Transfer ◽  
Lymph Node Cells ◽  
Autoimmune Myasthenia ◽  
Experimental Autoimmune

Passive transfer of experimental autoimmune myasthenia (EAM) was performed with lymph node cells from donor guinea pigs immunized with purified acetylcholine receptor (AChR) from Torpedo californica. Recipient animals revealed the same clinical signs and electromyographic patterns as observed in actively challenged animals. These phenomena are parallel to the clinical manifestations of the human disease myasthenia gravis, in which cellular response to AChR was recently demonstrated.

Immune response gene polymorphisms in tuberculosis

2015 ◽  
Vol 62 (4) ◽  
pp. 633-640 ◽  
Author(s):  
Marek Fol ◽  
Magdalena Druszczynska ◽  
Marcin Wlodarczyk ◽  
Elzbieta Ograczyk ◽  
Wieslawa Rudnicka
Keyword(s):  
Immune Response ◽  
Gene Polymorphisms ◽  
Immune Response Gene ◽  
Response Gene

scholarly journals RECEPTORS ON IMMUNOCOMPETENT CELLS

1971 ◽  
Vol 134 (2) ◽  
pp. 517-531 ◽  
Author(s):  
Joseph M. Davie ◽  
Alan S. Rosenthal ◽  
William E. Paul
Keyword(s):  
Immune Response ◽  
Guinea Pig ◽  
Heavy Chain ◽  
Guinea Pigs ◽  
Bovine Serum ◽  
Antigen Binding ◽  
Agarose Beads ◽  
Cellular Immune ◽  
Draining Lymph Nodes ◽  
Antibody Secreting Cells

Guinea pigs immunized with 2,4-dinitrophenyl-guinea pig albumin (DNP-GPA) possess lymphocytes which specifically bind sufficient DNP-GPA-125I to their surface to be detected by radioautography. These lymphocytes are present in the draining lymph nodes in a frequency of ∼50/1000 lymphocytes in animals immunized 2–4 wk earlier with DNP-GPA in complete Freund's adjuvant. Nonimmunized animals have ∼0.4 DNP-GPA antigen-binding cells (ABC) per 1000 lymphocytes. An increase in the frequency of DNP-GPA ABC in peripheral blood is detectable by 5 days after immunization, which is before the time that serum anti-DNP antibody is measurable. The receptors of these ABC are hapten specific in that free ϵ-DNP-L-lysine, at low concentration, inhibits the binding of DNP-GPA-125I; DNP bovine serum alumbin (DNP-BSA) is equivalent to DNP-GPA in the inhibition of binding of DNP-GPA-125I to ABC; and both DNP-GPA agarose beads and DNP-BSA agarose beads specifically adsorb DNP-GPA-125I ABC. Anti-immunoglobulin antisera, particularly anti-γ2 sera, inhibit the binding of DNP-GPA-125I to these cells implying that the receptors are immunoglobulin, primarily of the γ2 heavy chain class. DNP-GPA-125I ABC appear to represent precursors of antibody-secreting cells and have specificity characteristics which are very different from cells, of similarly immunized guinea pigs, which mediate a cellular immune response to DNP-GPA.

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