scholarly journals LYMPHOCYTE ACTIVATION IN SUBACUTE SCLEROSING PANENCEPHALITIS VIRUS AND CYTOMEGALOVIRUS INFECTIONS

1973 ◽  
Vol 138 (4) ◽  
pp. 839-846 ◽  
Author(s):  
G. B. Thurman ◽  
A. Ahmed ◽  
D. M. Strong ◽  
R. C. Knudsen ◽  
W. R. Grace ◽  
...  
Keyword(s):  
Measles Virus ◽  
Subacute Sclerosing Panencephalitis ◽  
Lymphocyte Activation ◽  
Virus Diseases ◽  
Sspe Virus ◽  
Slow Virus ◽  
Immune Capacity ◽  
Slow Virus Diseases ◽  
Cytomegalovirus Infections

Efforts to stimulate lymphocytes from measles seropositive and two patients with subacute sclerosing panencephalitis (SSPE) with either commercially available measles virus or virus isolated from a known case of SSPE failed to show any significant data using a microculture assay. Similar results were obtained using lymphocytes from two patients with active cytomegalovirus (CMV) infections and CMV seropositive individuals using CMV suspensions. On the other hand, lymphocytes from the patients with subacute sclerosing panencephalitis exhibited in vitro blastogenesis in culture with SSPE virus-infected HeLa cells. Similarly, lymphocytes from the CMV-infected patients demonstrated blastogenesis when cocultivated with CMV-infected WI-38 cells. This affords a new method for determining the cell-mediated immune capacity of patients with "slow" virus diseases.

Use of Protein a Conjugated to Peroxidase to Localize Immunoglobulin G in SSPE Ferret Brain

1982 ◽  
Vol 40 ◽  
pp. 350-351
Author(s):  
Hannah R. Brown ◽  
Anthony F. Nostro ◽  
Halldor Thormar
Keyword(s):  
Immunoglobulin G ◽  
Human Disease ◽  
Measles Virus ◽  
Subacute Sclerosing Panencephalitis ◽  
Protein A ◽  
Inflammatory Lesions ◽  
Sspe Virus ◽  
Specific Immunoglobulin ◽  
Antibody Titers ◽  
The Brain

Subacute sclerosing panencephalitis (SSPE) is a slowly progressing disease of the CNS in children which is caused by measles virus. Ferrets immunized with measles virus prior to inoculation with the cell associated, syncytiogenic D.R. strain of SSPE virus exhibit characteristics very similar to the human disease. Measles virus nucleocapsids are present, high measles antibody titers are found in the sera and inflammatory lesions are prominent in the brains. Measles virus specific immunoglobulin G (IgG) is present in the brain,and IgG/ albumin ratios indicate that the antibodies are synthesized within the CNS.

Infection-specific particle from the unconventional slow virus diseases

Science ◽  
1984 ◽  
Vol 225 (4660) ◽  
pp. 437-440 ◽  
Author(s):  
P. Merz ◽  
R. Rohwer ◽  
R Kascsak ◽  
H. Wisniewski ◽  
R. Somerville ◽  
...  
Keyword(s):  
Virus Diseases ◽  
Slow Virus ◽  
Slow Virus Diseases ◽  
Specific Particle

Interspecies Patterns of Slow Virus Diseases

1972 ◽  
Vol 23 (1) ◽  
pp. 191-200 ◽  
Author(s):  
R W Leader ◽  
A I Hurvitz
Keyword(s):  
Virus Diseases ◽  
Slow Virus ◽  
Slow Virus Diseases

scholarly journals High-Dose Intravenous Ribavirin Therapy for Subacute Sclerosing Panencephalitis

2001 ◽  
Vol 45 (3) ◽  
pp. 943-945 ◽  
Author(s):  
Mitsuaki Hosoya ◽  
Shiro Shigeta ◽  
Shuichi Mori ◽  
Akemi Tomoda ◽  
Seiji Shiraishi ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Virus Replication ◽  
Subacute Sclerosing Panencephalitis ◽  
Alpha Interferon ◽  
High Dose ◽  
Sspe Virus ◽  
High Doses

ABSTRACT Two patients with subacute sclerosing panencephalitis (SSPE) were treated safely and effectively with high doses of intravenous ribavirin combined with intraventricular alpha interferon. The ribavirin concentrations maintained in the serum and cerebrospinal fluid were higher than those which inhibit SSPE virus replication in vitro and in vivo.

scholarly journals RNA editing enzyme adenosine deaminase is a restriction factor for controlling measles virus replication that also is required for embryogenesis

2010 ◽  
Vol 108 (1) ◽  
pp. 331-336 ◽  
Author(s):  
Simone V. Ward ◽  
Cyril X. George ◽  
Megan J. Welch ◽  
Li-Ying Liou ◽  
Bumsuk Hahm ◽  
...  
Keyword(s):  
Adenosine Deaminase ◽  
Virus Replication ◽  
Measles Virus ◽  
Influenza A ◽  
Sendai Virus ◽  
Subacute Sclerosing Panencephalitis ◽  
Lymphocyte Activation ◽  
Syncytium Formation ◽  
Restriction Factor

Measles virus (MV), a member of the family Paramyxoviridae and an exclusively human pathogen, is among the most infectious viruses. A progressive fatal neurodegenerative complication, subacute sclerosing panencephalitis (SSPE), occurs during persistent MV infection of the CNS and is associated with biased hypermutations of the viral genome. The observed hypermutations of A-to-G are consistent with conversions catalyzed by the adenosine deaminase acting on RNA (ADAR1). To evaluate the role of ADAR1 in MV infection, we selectively disrupted expression of the IFN-inducible p150 ADAR1 isoform and found it caused embryonic lethality at embryo day (E) 11–E12. We therefore generated p150-deficient and WT mouse embryo fibroblast (MEF) cells stably expressing the MV receptor signaling lymphocyte activation molecule (SLAM or CD150). The p150−/−but not WT MEF cells displayed extensive syncytium formation and cytopathic effect (CPE) following infection with MV, consistent with an anti-MV role of the p150 isoform of ADAR1. MV titers were 3 to 4 log higher in p150−/−cells compared with WT cells at 21 h postinfection, and restoration of ADAR1 in p150−/−cells prevented MV cytopathology. In contrast to infection with MV, p150 disruption had no effect on vesicular stomatitis virus, reovirus, or lymphocytic choriomeningitis virus replication but protected against CPE resulting from infection with Newcastle disease virus, Sendai virus, canine distemper virus, and influenza A virus. Thus, ADAR1 is a restriction factor in the replication of paramyxoviruses and orthomyxoviruses.

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