scholarly journals In vitro studies on lymphocyte differentiation. I. Long term in vitro culture of cells giving rise to functional lymphocytes in irradiated mice.

1978 ◽  
Vol 148 (3) ◽  
pp. 823-828 ◽  
Author(s):  
J W Schrader ◽  
S Schrader
Keyword(s):  
B Lymphocytes ◽  
Cultured Cells ◽  
Bone Marrow Cells ◽  
In Vitro Cultures ◽  
Mouse Bone Marrow ◽  
Graft Versus Host ◽  
Receptor Repertoire ◽  
Mouse Bone Marrow Cells

In vitro cultures of mouse bone marrow cells, maintained for periods up to 7 wk, were shown to contain cells able to repopulate irradiated hosts with T and B lymphocytes. The lymphocytes were fully functional and there did not appear to be any gross restriction of their receptor repertoire. The cultured cells reconstituted irradiated semiallogenic hosts without evidence of graft-versus-host disease, suggesting that culture of donor marrow might be a useful preliminary to transplantation when tissue matching is incomplete.

scholarly journals CONTRIBUTION OF A THYMIC HUMORAL FACTOR TO THE DEVELOPMENT OF AN IMMUNOLOGICALLY COMPETENT POPULATION FROM CELLS OF MOUSE BONE MARROW

1971 ◽  
Vol 134 (3) ◽  
pp. 786-800 ◽  
Author(s):  
Myra Small ◽  
Nathan Trainin
Keyword(s):  
Bone Marrow ◽  
Lymphoid Tissue ◽  
Host Response ◽  
Bone Marrow Cells ◽  
Immune Reactivity ◽  
Mouse Bone Marrow ◽  
Graft Versus Host ◽  
Peripheral Lymphoid Tissue ◽  
Marrow Cells

The hypothesis that cells located in mouse bone marrow can acquire immunological competence by a process that involves interaction with a noncellular component of the thymus was tested using an in vitro assay of graft-versus-host reactivity as a criterion of cell competence. When suspensions of C57BL bone marrow cells were incubated in thymus extract and injected into mice incapable of inducing a response in the graft-versus-host assay as a result of neonatal thymectomy, or adult thymectomy plus irradiation, or because of genetic similarity with the (C3H x C57BL)F1 tissue used for challenge in the assay, competent cells were recovered from the spleens of the injected mice. The reactive cells were shown to be of bone marrow origin since immune reactivity was related to the genetic makeup of the bone marrow cells rather than that of the intermediate recipients. A thymic factor was involved in the process leading to immune reactivity by these cells, as bone marrow cells incubated in xenogeneic or syngeneic thymic extracts induced a graft-versus-host response after passage through nonresponsive mice, whereas incubation of bone marrow cells in xenogeneic lymph node or spleen extracts or in culture medium only did not lead to subsequent reactivity. Participation of peripheral lymphoid tissue seemed essential in this process since bone marrow cells tested directly after exposure to thymic extract failed to induce a graft-versus-host response. C57BL bone marrow cells exposed to thymus extract and cultured together with fragments of (C3H x C57BL)F1 spleen tissue in vitro were competent to induce a graft-versus-host response; thus, these components would seem to be sufficient as well as necessary for the immunodifferentiation process leading to graft-versus-host activity. It is concluded that one step in the process by which bone marrow cells acquire competence vis-a-vis the graft-versus-host response depends upon a thymic agent that is noncellular and extractable, and that another stage in this process is under the influence of components found within the peripheral lymphoid tissue environment. It is suggested that differentiation of precursor cells to competence could occur by progressive development of the cells in separate compartments of the lymphoid system.

Malignant Transformation of Mouse Bone Marrow Cells In Vitro Induced by 60 Co γ-Ray Irradiation

1984 ◽  
pp. 830-830
Author(s):  
F. M. Gao ◽  
X. L. Li ◽  
M. J. Qian ◽  
W. H. Wang ◽  
F. Q. Qi ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Malignant Transformation ◽  
Bone Marrow Cells ◽  
Mouse Bone Marrow ◽  
Γ Ray ◽  
Mouse Bone Marrow Cells ◽  
Marrow Cells

The effect of decitabine on megakaryocyte maturation and platelet release

2011 ◽  
Vol 106 (08) ◽  
pp. 337-343 ◽  
Author(s):  
Jianhui Wang ◽  
Zanhua Yi ◽  
Shiyang Wang ◽  
Zongdong Li
Keyword(s):  
Bone Marrow ◽  
Platelet Count ◽  
Bone Marrow Cells ◽  
Mouse Bone Marrow ◽  
Human Cord Blood ◽  
Primary Mouse ◽  
Platelet Release ◽  
Mouse Bone Marrow Cells ◽  
Marrow Cells

SummaryThrombocytopenia is a common feature of myelodysplastic syndromes (MDS). 5-aza-2’-deoxycytidine (decitabine) has been used to treat MDS with an approximately 20% response rate in thrombocytopenia. However, the mechanism of how decitabine increases platelet count is not clear. In this study, we investigated the effect of decitabine on megakaryocyte maturation and platelet release in the mouse. The effect of decitabine on megakaryocyte maturation was studied in an in vitro megakaryocyte differentiation model utilising mouse bone marrow cells and mouse megakaryoblastic cell line L8057. Decitabine (2.5 μM) is able to induce L8057 cells to differentiate into a megakaryocyte-like polyploidy cells with positive markers of acetylcholinesterase and αIIb integrin (CD41). Higher expression of αIIb integrin was also found in primary mouse bone marrow cells and human cord blood CD34+ cells cultured with both thrombopoietin and decitabine as compared to thrombopoietin alone. In addition, we noted a 30% platelet count increase in Balb/c mice 12 hours after the injection of decitabine at a clinically relevant dose (15 mg/m2), suggesting a rapid platelet release from the spleen or bone marrow. Our data suggest that decitabine increases platelet counts by enhancing platelet release and megakaryocyte maturation.

In vitro effects of OK-432 on irradiated mouse bone marrow cells

1994 ◽  
Vol 29 (3) ◽  
pp. 631-634 ◽  
Author(s):  
Masako Nose ◽  
Yoshiro Aoki ◽  
Yoshiko Kawase ◽  
Gen Suzuki ◽  
Makoto Akashi ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Mouse Bone Marrow ◽  
In Vitro Effects ◽  
Mouse Bone Marrow Cells ◽  
Marrow Cells

scholarly journals A comparative study of the effects of genistein, estradiol and raloxifene on the murine skeletal system.

2009 ◽  
Vol 56 (2) ◽  
Author(s):  
Leszek Sliwiński ◽  
Joanna Folwarczna ◽  
Barbara Nowińska ◽  
Urszula Cegieła ◽  
Maria Pytlik ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Bone Marrow ◽  
Mrna Expression ◽  
Bone Marrow Cells ◽  
Bone Mineralization ◽  
Mouse Bone Marrow ◽  
Skeletal System ◽  
Mouse Bone Marrow Cells

Genistein, a major phytoestrogen of soy, is considered a potential drug for prevention and treatment of postmenopausal osteoporosis. The aim of the present study was to compare the effects of genistein, estradiol and raloxifene on the skeletal system in vivo and in vitro. Genistein (5 mg/kg), estradiol (0.1 mg/kg) or raloxifene hydrochloride (5 mg/kg) were administered daily by a stomach tube to mature ovariectomized Wistar rats for 4 weeks. Bone mass, mineral and calcium content, macrometric parameters and mechanical properties were examined. Also the effects of genistein, estradiol and raloxifene (10(-9)-10(-7) M) on the formation of osteoclasts from neonatal mouse bone marrow cells and the activity of osteoblasts isolated from neonatal mouse calvariae were compared. In vivo, estrogen deficiency resulted in the impairment of bone mineralization and bone mechanical properties. Raloxifene but not estradiol or genistein improved bone mineralization. Estradiol fully normalized the bone mechanical properties, whereas genistein augmented the deleterious effect of estrogen-deficiency on bone strength. In vitro, genistein, estradiol and raloxifene inhibited osteoclast formation from mouse bone marrow cells, decreasing the ratio of RANKL mRNA to osteoprotegerin mRNA expression in osteoblasts. Genistein, but not estradiol or raloxifene, decreased the ratio of alkaline phosphatase mRNA to ectonucleotide pyrophosphatase phosphodiesterase 1 mRNA expression in osteoblasts. This difference may explain the lack of genistein effect on bone mineralization observed in ovariectomized rats in the in vivo study. Concluding, our experiments demonstrated profound differences between the activities of genistein, estradiol and raloxifene towards the osseous tissue in experimental conditions.

A Conditional Fibroblast Growth Factor Receptor-1 Derivative Stimulates Extensive Self Renewal of Genetically Modified Primary Hematopoietic Cells.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 493-493
Author(s):  
Ingrid Lintmaer ◽  
Michael A. Weinreich ◽  
C. Anthony Blau
Keyword(s):  
Fibroblast Growth Factor Receptor ◽  
Bone Marrow Cells ◽  
Genetically Modified ◽  
Hematopoietic Cells ◽  
Growth Factor Receptor ◽  
Mouse Bone Marrow ◽  
Fibroblast Growth ◽  
Self Renewal ◽  
Mouse Bone Marrow Cells

Abstract Fibroblast growth factor receptor-1 (FGFR-1) signaling has been implicated in self renewal and proliferation of hematopoietic stem cells. We have studied the effect of a conditional FGFR-1 in genetically modified primary mouse bone marrow cells. The activity of the conditional FGFR-1 is dependent on the presence of a drug called a chemical inducer of dimerization (CID). For these studies we used a bicistronic vector encoding green fluorescence protein (GFP) as a marker and a fusion protein made up of the intracellular portion of FGFR-1 and the CID binding domain. To test the construct in vitro we plated transduced mouse bone marrow cells in 10% serum with or without the CID, AP20187 (100 nM). While the cells without CID died within two weeks, in the culture with CID the transduced, GFP marked cells proliferated. These hematopoietic cells have proliferated in vitro for over 30 weeks. This makes FGFr-1 only the second CID dependent receptor, other than mpl, that is able to support long-term self renewal of primary hematopoietic cells. By day 28 of culture cells had expanded from 106 to 1.2x1012 cells. The differentiation potential of these cells was tested by transplanting 8 million cells into each of 5 lethally irradiated (1050 cGy) congenic recipients. Four mice are alive 76 days post transplantation, with mean frequencies of GFP positive red cells, platelets, granulocytes, monocytes, B cells and T cells of 55%, 5%, 85%, 98%, 42% and 2%, respectively. These findings indicate that cells expanded under the influence of FGFR-1 signaling retain both lymphoid and myeloid repopulating ability. Additionally, CID-mediated activation of FGFR-1 induced a dramatic rise in genetically modfied red cells, granulocytes and monocytes in vivo. These findings point to a clear difference between CID induced activation of FGFR-1 compared to our previous studies using the thrombopoietin receptor, where effects on neutrophils and monocytes are modest and variable. The CID controlled activation of FGFR-1 may have utility for control of genetically modified hematopoietic cells.

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