scholarly journals Hapten-specific T cell responses to 4-hydroxy-3-nitrophenyl acetyl. VI. Evidence for different T cell receptors in cells that mediate H-21-restricted and H-2D-restricted cutaneous sensitivity responses.

1980 ◽  
Vol 152 (6) ◽  
pp. 1554-1562 ◽  
Author(s):  
M E Sunday ◽  
B Benacerraf ◽  
M E Dorf
Keyword(s):  
T Cell ◽  
T Cell Receptors ◽  
Inbred Strains ◽  
Cross Reactivity ◽  
Delayed Type Hypersensitivity ◽  
Cell Receptors ◽  
Cell Responses ◽  
Histocompatibility Complex ◽  
Inbred Strains Of Mice ◽  
Lymph Node Cells

We have previously shown that cross-reactive sensitivity (CS) responses induced by 4-hydroxy-3-nitrophenyl acetyl-O-succinimide (NP-O-Su) and elicited by its 5-iodo analogue, 4-hydroxy-5-iodo-3-nitrophenyl acetyl-O-succinimide were observed in strains of mice possessing the Igh-1b allotype, but not in strains bearing allotypes Igh-1c or Igh-1j. These CS responses are mediated by T cells and can be transferred to naive recipients that are homologous at either the H-2K, H-2I, or H-2D regions of the major histocompatibility complex. We now extend our analysis of cross-reactive 4-hydroxy-3-nitrophenyl-acetyl (NP)-induced CS responses to inbred strains of mice expressing additional Igh-1 allotypes. In contrast to NP-induced delayed-type hypersensitivity responses, which only display 4-hydroxy-5-iodo-3-nitrophenyl acetyl (NIP) cross-reactivity in Igh-1b-bearing mice, cross-reactive CS responses can also be elicited in NP-primed mice carrying the Igh-1d, Igh-1e, or Igh-1f allotypes. Moreover, cross-reactive NP-induced CS responses could be transferred by NP-O-Su-primed lymph node cells from the AKR (Igh-1d) strain, into naive recipients homologous at the H-2D region, but only non-cross-reactive NP responses could be transferred into strains homologous at the H-2I region. Furthermore, the lack of cross-reactivity in the Igh-1j-bearing C3H strain was not the result of an inability of these mice to recognize NP in association with H-2K/D products, because NP-O-Su-primed cells from C3H donors transferred NP-specific CS responses into both H-2D and H02I homologous recipients. The results are discussed with respect to the nature of the T cell receptors that control NP responses.

scholarly journals Identification of Cross-Reactive CD8+ T Cell Receptors with High Functional Avidity to a SARS-CoV-2 Immunodominant Epitope and Its Natural Mutant Variants

2020 ◽  
Author(s):  
Chao Hu ◽  
Meiying Shen ◽  
Xiaojian Han ◽  
Qian Chen ◽  
Luo Li ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Receptors ◽  
Vaccine Development ◽  
Ex Vivo ◽  
T Cell Responses ◽  
Cross Reactivity ◽  
Functional Avidity ◽  
Cell Receptors ◽  
Cell Functions ◽  
Cell Responses

ABSTRACTDespite the growing knowledge of T cell responses and their epitopes in COVID-19 patients, there is a lack of detailed characterizations for T cell-antigen interactions and T cell functions. Using a peptide library predicted with HLA class I-restriction, specific CD8+ T cell responses were identified in over 75% of COVID-19 convalescent patients. Among the 15 SARS-CoV-2 epitopes identified from the S and N proteins, N361-369 (KTFPPTEPK) was the most dominant epitope. Importantly, we discovered 2 N361-369-specific T cell receptors (TCRs) with high functional avidity, and they exhibited complementary cross-reactivity to reported N361-369 mutant variants. In dendritic cells (DCs) and the lung organoid model, we found that the N361-369 epitope could be processed and endogenously presented to elicit the activation and cytotoxicity of CD8+ T cells ex vivo. Our study evidenced potential mechanisms of cellular immunity to SARS-CoV-2, illuminating natural ways of viral clearance with high relevancy in the vaccine development.

scholarly journals Predicting Cross-Reactivity and Antigen Specificity of T Cell Receptors

2020 ◽  
Vol 11 ◽  
Author(s):  
Chloe H. Lee ◽  
Mariolina Salio ◽  
Giorgio Napolitani ◽  
Graham Ogg ◽  
Alison Simmons ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Receptors ◽  
Cross Reactivity ◽  
Antigen Specificity ◽  
Cell Receptors

scholarly journals Immunological studies of T-cell receptors. II. Limited polymorphism of idiotypic determinants on T-cell receptors specific for major histocompatibility complex alloantigens.

1979 ◽  
Vol 149 (1) ◽  
pp. 234-243 ◽  
Author(s):  
D Bellgrau ◽  
D B Wilson
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Receptors ◽  
Parental Strain ◽  
Germ Line ◽  
Third Party ◽  
Cell Receptors ◽  
Histocompatibility Complex ◽  
Cell Mediated Immune Response ◽  
Different Strains

These studies explore the extent of genetic polymorphism in the expression of anti-MHC receptors by T cells in different strains of rats. This question was approached with the use of the model of specifically induced GVH resistance in F1 rats which has been shown to reflect a specific T-cell mediated immune response against parental strain T cell anti-MHC receptors specific for host alloantigens. When A/B F1 rats derived from MHC incompatibile matings are immunized with lymphocytes from one parental strain (A they display a specific resistance to anti-B GVH reactivity caused by T cells from that parental strain, but not anti-AGVH reactions from the other. In addition, they resist anti-B GHV reactivity by T cells from third-party donors (C, D, E,...), a finding taken to indicate that the idiotypes of anti-MHC receptors on T cells, recognized by other T cells, show little or no polymorphism. This conclusion suggests that anti-MHC receptors are shared in the species and may be encoded, at least partially, by germ-line genes.

scholarly journals T-cell Receptors Engineered De Novo for Peptide Specificity Can Mediate Optimal T-cell Activity without Self Cross-Reactivity

2019 ◽  
Vol 7 (12) ◽  
pp. 2025-2035 ◽  
Author(s):  
Preeti Sharma ◽  
Daniel T. Harris ◽  
Jennifer D. Stone ◽  
David M. Kranz
Keyword(s):  
T Cell ◽  
T Cell Receptors ◽  
De Novo ◽  
Cell Activity ◽  
Cross Reactivity ◽  
Cell Receptors ◽  
Peptide Specificity

Antigen-Specific, Major Histocompatibility Complex-Restricted T Cell Receptors

1983 ◽  
Vol 76 (1) ◽  
pp. 131-145 ◽  
Author(s):  
P. Marrack ◽  
C. Hannum ◽  
M. Harris ◽  
K. Haskins ◽  
R. Kubo ◽  
...  
Keyword(s):  
Major Histocompatibility Complex ◽  
T Cell ◽  
T Cell Receptors ◽  
Major Histocompatibility ◽  
Cell Receptors ◽  
Histocompatibility Complex

scholarly journals The somatically generated portion of T cell receptor CDR3α contributes to the MHC allele specificity of the T cell receptor

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Philippa Marrack ◽  
Sai Harsha Krovi ◽  
Daniel Silberman ◽  
Janice White ◽  
Eleanor Kushnir ◽  
...  
Keyword(s):  
Major Histocompatibility Complex ◽  
T Cell ◽  
Positive Selection ◽  
T Cell Receptor ◽  
T Cell Receptors ◽  
Cell Receptor ◽  
Structural Basis ◽  
Major Histocompatibility ◽  
Cell Receptors ◽  
Histocompatibility Complex

Mature T cells bearing αβ T cell receptors react with foreign antigens bound to alleles of major histocompatibility complex proteins (MHC) that they were exposed to during their development in the thymus, a phenomenon known as positive selection. The structural basis for positive selection has long been debated. Here, using mice expressing one of two different T cell receptor β chains and various MHC alleles, we show that positive selection-induced MHC bias of T cell receptors is affected both by the germline encoded elements of the T cell receptor α and β chain and, surprisingly, dramatically affected by the non germ line encoded portions of CDR3 of the T cell receptor α chain. Thus, in addition to determining specificity for antigen, the non germline encoded elements of T cell receptors may help the proteins cope with the extremely polymorphic nature of major histocompatibility complex products within the species.

scholarly journals Analysis of SARS-CoV-2 specific T-cell receptors in ImmuneCode reveals cross-reactivity to immunodominant Influenza M1 epitope

2020 ◽  
Author(s):  
John-William Sidhom ◽  
Alexander S. Baras
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
T Cell Receptors ◽  
Cell Receptor ◽  
Cross Reactivity ◽  
Cell Receptors

Adaptive Biotechnologies and Microsoft have recently partnered to release ImmuneCode, a database containing SARS-CoV-2 specific T-cell receptors derived through MIRA, a T-cell receptor (TCR) sequencing based sequencing approach to identify antigen-specific TCRs. Herein, we query the extent of cross reactivity between these derived SARS-CoV-2 specific TCRs and other known antigens present in McPas-TCR, a manually curated catalogue of pathology-associated TCRs. We reveal cross reactivity between SARS-CoV-2 specific TCRs and the immunodominant Influenza GILGFVFTL M1 epitope, suggesting the importance of further work in characterizing the implications of prior Influenza exposure or co-exposure to the pathology of SARS-CoV-2 illness.

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