scholarly journals Evidence of widespread binding of HLA class I molecules to peptides.

1990 ◽  
Vol 172 (3) ◽  
pp. 827-834 ◽  
Author(s):  
J A Frelinger ◽  
F M Gotch ◽  
H Zweerink ◽  
E Wain ◽  
A J McMichael
Keyword(s):  
Monoclonal Antibodies ◽  
Binding Assay ◽  
Solid Phase ◽  
Hla Class I ◽  
Class I ◽  
T Cell Epitopes ◽  
Single Class ◽  
Hla Class I Molecules ◽  
Solid Phase Binding ◽  
Hiv 1

We have tested the binding of HLA class I proteins to peptides using a solid-phase binding assay. We tested 102 peptides, mostly derived from the HIV gag and HIV pol sequences. Most peptides did not bind to any class I protein tested. The pattern of binding among the three class I proteins tested, HLA-A2, -B27, and -B8, was approximately 85% concordant. Further, all five of the known HIV-1 gag T cell epitopes detected by human CTL bound at least one class I protein. Binding of class I to the peptides could be detected either by directly iodinated class I proteins, or indirectly using monoclonal antibodies specific for class I. The binding to the plates could be blocked with MA2.1, which binds in the alpha 1 region of A2, but not by W6/32, which binds elsewhere. The data presented here show that binding of class I to peptides is specific, but that many peptides bind to more than a single class I protein.

scholarly journals Favorable and Unfavorable HLA Class I Alleles and Haplotypes in Zambians Predominantly Infected with Clade C Human Immunodeficiency Virus Type 1

2002 ◽  
Vol 76 (16) ◽  
pp. 8276-8284 ◽  
Author(s):  
Jianming Tang ◽  
Shenghui Tang ◽  
Elena Lobashevsky ◽  
Angela D. Myracle ◽  
Ulgen Fideli ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Hla Class I ◽  
Class I ◽  
Hla Class I Molecules ◽  
Immunodeficiency Virus ◽  
Clade C ◽  
Hiv 1

ABSTRACT The setpoint of viral RNA concentration (viral load [VL]) during chronic human immunodeficiency virus type 1 (HIV-1) infection reflects a virus-host equilibration closely related to CD8+ cytotoxic T-lymphocyte (CTL) responses, which rely heavily on antigen presentation by the human major histocompatibility complex (MHC) (i.e., HLA) class I molecules. Differences in HIV-1 VL among 259 mostly clade C virus-infected individuals (137 females and 122 males) in the Zambia-UAB HIV Research Project (ZUHRP) were associated with several HLA class I alleles and haplotypes. In particular, general linear model analyses revealed lower log10 VL among those with HLA allele B*57 (P = 0.002 [without correction]) previously implicated in favorable response and in those with HLA B*39 and A*30-Cw*03 (P = 0.002 to 0.016); the same analyses also demonstrated higher log10 VL among individuals with A*02-Cw*16, A*23-B*14, and A*23-Cw*07 (P = 0.010 to 0.033). These HLA effects remained strong (P = 0.0002 to 0.075) after adjustment for age, gender, and duration of infection and persisted across three orders of VL categories (P = 0.001 to 0.084). In contrast, neither B*35 (n = 15) nor B*53 (n = 53) showed a clear disadvantage such as that reported elsewhere for these closely related alleles. Other HLA associations with unusually high (A*68, B*41, B*45, and Cw*16) or low (B*13, Cw*12, and Cw*18) VL were either unstable or reflected their tight linkage respecting disequilibria with other class I variants. The three consistently favorable HLA class I variants retained in multivariable models and in alternative analyses were present in 30.9% of subjects with the lowest (<10,000 copies per ml) and 3.1% of those with the highest (>100,000) VL. Clear differential distribution of HLA profiles according to level of viremia suggests important host genetic contribution to the pattern of immune control and escape during HIV-1 infection.

Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules

2003 ◽  
Vol 64 (3) ◽  
pp. 315-326 ◽  
Author(s):  
Catherine Menier ◽  
Berta Saez ◽  
Vaclav Horejsi ◽  
Silvia Martinozzi ◽  
Irene Krawice-Radanne ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Hla Class I ◽  
Class I ◽  
Hla Class I Molecules

3.1-04 Production of monoclonal antibodies reactive with the new HLA class I molecules

1989 ◽  
Vol 26 ◽  
pp. 16
Author(s):  
D Geraghty ◽  
S Orcutt ◽  
T Staggs ◽  
J Hansen
Keyword(s):  
Monoclonal Antibodies ◽  
Hla Class I ◽  
Class I ◽  
Hla Class I Molecules

Effects of HIV-1 Tat on Expression of HLA Class I Molecules

1996 ◽  
Vol 11 (3) ◽  
pp. 233-240 ◽  
Author(s):  
Masanori Matsui ◽  
Robert J. Warburton ◽  
Patricia C. Cogswell ◽  
Albert S. Baldwin ◽  
Jeffrey A. Frelinger
Keyword(s):  
Hla Class I ◽  
Class I ◽  
Hla Class I Molecules ◽  
Hiv 1

scholarly journals Platelet activation by cross-linking HLA class I molecules and Fc receptor

Blood ◽  
1992 ◽  
Vol 79 (11) ◽  
pp. 2901-2908 ◽  
Author(s):  
E Rubinstein ◽  
I Urso ◽  
C Boucheix ◽  
RC Carroll
Keyword(s):  
Monoclonal Antibodies ◽  
Platelet Activation ◽  
Hla Class I ◽  
Fc Receptor ◽  
Class I ◽  
Cross Linking ◽  
Chain Molecule ◽  
Kinase C ◽  
Hla Class I Molecules ◽  
Higher Sensitivity

Abstract The effect on platelet activation of monoclonal antibodies directed against common determinants of the HLA class I heavy chain molecule was studied. Cross-linking W6/32, an anti-HLA class I of IgG2a subclass, led to platelet activation. Two other antibodies of the same subclass did not have this effect on platelets. The lack of activity of the F(ab')2 fragments suggests that the activation signal is mediated by the platelet Fc receptor (Fc gamma RII). Indeed, except for a higher sensitivity of W6/32 to aspirin and apyrase, activations by cross- linking IV-3 (an anti-Fc gamma RII) and W6/32 are similar at the level of InsP3 formation, calcium mobilization, pH modifications, and activation of protein kinase C and myosin kinase. When HLA class I molecules and Fc gamma RII are cross-linked together, platelet activation occurs. This is not observed when a control IgG2a is substituted for W6/32 or when CD9 and Fc receptor are cross-linked together. This suggests that HLA class I molecules and Fc gamma RII synergize to activate platelets.

scholarly journals Analysis of transporter associated with antigen presentation (TAP) genes polymorphisms with HIV-1 infection

2019 ◽  
Vol 464 (1-2) ◽  
pp. 65-71 ◽  
Author(s):  
Abaineh Munshea Abitew ◽  
Ranbir Chander Sobti ◽  
Vijay Lakshmi Sharma ◽  
Ajay Wanchu
Keyword(s):  
Immune Response ◽  
Hla Class I ◽  
Class I ◽  
Control Analysis ◽  
Antigenic Peptides ◽  
Human Major Histocompatibility Complex ◽  
Hla Class I Molecules ◽  
Increased Risk ◽  
Exon 11 ◽  
Hiv 1

AbstractHuman leukocyte antigen (HLA) class I molecules of the human major histocompatibility complex (MHC) play an important role in modulating immune response. HLA class I molecules present antigenic peptides to CD8+ T cells and thereby play a role in the immune surveillance of cells infected with viruses. TAP1 and TAP2 are MHC-II-encoded genes necessary for the generation of a cellular immune response and polymorphism of these genes can influence the specificity of peptides preferentially presented by the MHC class I molecules and the outcome of the immune response. Several studies implicated genetic variation in TAP genes to various immune-mediated and infectious diseases. To determine the correlation between HIV-1 infection and the TAP1 and TAP2 genes polymorphisms, we performed PCR–RFLP assay of these genes in 500 HIV-1 seropositives and the matched seronegative individuals. Statistical analysis of the data disclosed no correlation between TAP1 (C/T intron 7) gene polymorphism and HIV-1/AIDS disease. However, the current results demonstrated that the heterozygous A/G [OR (95% CI) 1.39 (1.06–1.83), P = 0.0171] and homozygous G/G [OR (95% CI) 3.38(1.56–7.46), P = 0.0010] variants of TAP2 (A/G exon 11) (T665A) gene are positively associated with an increased risk of HIV-1/AIDS infection. This case–control analysis might suggest a possible role of TAP2 (A/G exon 11) (T665A) gene in the susceptibility to HIV-1 infection and disease outcome among North Indian patients.

scholarly journals Adaptive Interactions between HLA and HIV-1: Highly Divergent Selection Imposed by HLA Class I Molecules with Common Supertype Motifs

2010 ◽  
Vol 184 (8) ◽  
pp. 4368-4377 ◽  
Author(s):  
Mina John ◽  
David Heckerman ◽  
Ian James ◽  
Lawrence P. Park ◽  
Jonathan M. Carlson ◽  
...  
Keyword(s):  
Hla Class I ◽  
Divergent Selection ◽  
Class I ◽  
Hla Class I Molecules ◽  
Hiv 1
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