scholarly journals STUDIES ON THE PROPAGATION IN VITRO OF POLIOMYELITIS VIRUSES

1952 ◽  
Vol 96 (4) ◽  
pp. 389-400 ◽  
Author(s):  
William F. Scherer ◽  
Jerome T. Syverton
Keyword(s):  
Virus Type ◽  
Testicular Tissue ◽  
Nerve Cells ◽  
Specific Antibodies ◽  
Poliomyelitis Virus ◽  
Explant Cultures ◽  
Pure Cultures ◽  
Primary Explant

Cells like fibroblasts, having no resemblance whatever to nerve cells were obtained in morphologically pure cultures from monkey testicular tissue and were found to support the growth in vitro of poliomyelitis virus, Type 2, Yale-SK strain. Moreover, these cells were destroyed as a result of the multiplication of this virus within them. Similarly, "fibroblasts" propagated in primary explant cultures of testicle were destroyed by poliomyelitis viruses, Types 1 and 2. Type specific antibodies neutralized the pathogenic effect of poliomyelitis virus on monkey testicular fibroblasts.

scholarly journals STUDIES ON THE PROPAGATION IN VITRO OF POLIOMYELITIS VIRUSES

1952 ◽  
Vol 96 (4) ◽  
pp. 369-388 ◽  
Author(s):  
William F. Scherer ◽  
Jerome T. Syverton
Keyword(s):  
Liquid Phase ◽  
Growth Curves ◽  
Testicular Tissue ◽  
Growth Cycle ◽  
Lag Phase ◽  
Viral Production ◽  
Poliomyelitis Virus ◽  
Viral Multiplication

The growth of poliomyelitis virus, Type 2, Yale-SK strain, in cultures of monkey testicular tissue was observed to occur in discrete cycles. Growth curves showed that each cycle was composed of (a) an initial lag phase when little or no virus was released from the cells, (6) a phase of viral production, and (c) a plateau which represented a decrement in the rate of viral production. This pattern of viral multiplication occurred in monkey testicular tissue cultures which have as the liquid phase either ox serum ultrafiltrate or monkey serum-chicken embryonic extract medium. The presence of a solid medium composed of chicken plasma, clotted either with chicken embryonic extract or bovine thrombin, did not alter the pattern of viral multiplication. The shape of the growth curve as established by any of four different techniques for tissue cultivation, was shown to be independent of the cultural technique employed. For cultures of monkey testicular tissue, the amount of virus in the tissue was as much as tenfold greater than that in the liquid of the same cultures. Moreover, viral production was evident earlier and was detectable for a longer period of time in the tissue than in the liquid phase. The rapidly incremental phase of the growth cycle, when large quantities of virus were released into the liquid phase, coincided in time with the destruction of the spindle-shaped cells, which extended from the explants. Although destruction of outgrowth cells was marked, there remained cells within the explants capable of supporting the growth of poliomyelitis virus.

The effect of prostaglandins on the multiplication and cell-to-cell spread of herpes simplex virus type 2 in vitro

1982 ◽  
Vol 144 (3) ◽  
pp. 346-349 ◽  
Author(s):  
David A. Baker ◽  
Joanne Thomas ◽  
Judy Epstein ◽  
Dominic Possilico ◽  
Martin L. Stone
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Simplex Virus ◽  
Cell Spread

In-vitro effect of human cathelicidin antimicrobial peptide LL-37 on dengue virus type 2

Peptides ◽  
2017 ◽  
Vol 92 ◽  
pp. 23-30 ◽  
Author(s):  
K. Alagarasu ◽  
P.S. Patil ◽  
P. Shil ◽  
M. Seervi ◽  
M.B. Kakade ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Antimicrobial Peptide ◽  
Virus Type ◽  
Vitro Effect ◽  
Dengue Virus Type 2

scholarly journals Inability to Detect Human T Cell Lymphotropic Virus Type 2-Specific Antibodies in a Patient Coinfected with HIV-1, Human T Cell Lymphotropic Virus Type 1, Human T Cell Lymphotropic Virus Type 2, and Hepatitis C Virus

2014 ◽  
Vol 30 (1) ◽  
pp. 97-101 ◽  
Author(s):  
Adele Caterino-de-Araujo ◽  
Mariana Cavalheiro Magri ◽  
Neuza Satomi Sato ◽  
Helena Kaminami Morimoto ◽  
Luis Fernando de Macedo Brigido ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
T Cell ◽  
Virus Type ◽  
Specific Antibodies ◽  
Human T Cell ◽  
Hiv 1

scholarly journals STUDIES ON THE PROPAGATION IN VITRO OF POLIOMYELITIS VIRUSES

1952 ◽  
Vol 96 (4) ◽  
pp. 355-367 ◽  
Author(s):  
Jerome T. Syverton ◽  
William F. Scherer
Keyword(s):  
Tissue Culture ◽  
Plasma Clot ◽  
Poliomyelitis Virus ◽  
Histopathological Findings ◽  
Testicular Cells ◽  
Fragment Type ◽  
Tissue Fragment ◽  
Culture Virus

Poliomyelitis virus was propagated in vitro successfully in extraneural tissues. Suspended tissue fragment cultures and combined plasma clot-suspended tissue fragment cultures of monkey or human testicular tissues were employed. Five strains representative of poliomyelitis virus were maintained for from 36 to 263 days in the suspended tissue fragment type of culture. The dilution factors calculated by tissue replacements for the eight serial passages ranged from 107.8 to 1044.5 and when assessed by fluid replacements, from 1015 to 1095.3. The LD50 for each strain of Type 2 virus was determined for selected transfers. The identify of each strain of virus was established by neutralization tests and histopathological findings in monkeys dead from the injection of tissue culture virus. Control experiments and other tests made known that propagation of poliomyelitis virus did not occur in the absence of viable testicular cells and that an extraneous virus was not inadvertently acquired during the course of these studies.

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