Efficacy of Catharanthus roseus Extract against Dengue Virus Type 2 Infection in Vitro

2020 ◽  
Vol 11 (2) ◽  
pp. 1583
Author(s):  
Noor Zarina Abd Wahab ◽  
NorefrinaShafinaz Md. Nor ◽  
Nazlina Ibrahim
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Catharanthus Roseus ◽  
Dengue Virus Type 2

In-vitro effect of human cathelicidin antimicrobial peptide LL-37 on dengue virus type 2

Peptides ◽  
2017 ◽  
Vol 92 ◽  
pp. 23-30 ◽  
Author(s):  
K. Alagarasu ◽  
P.S. Patil ◽  
P. Shil ◽  
M. Seervi ◽  
M.B. Kakade ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Antimicrobial Peptide ◽  
Virus Type ◽  
Vitro Effect ◽  
Dengue Virus Type 2

scholarly journals Infectious clone construction of dengue virus type 2, strain Jamaican 1409, and characterization of a conditional E6 mutation

2006 ◽  
Vol 87 (8) ◽  
pp. 2263-2268 ◽  
Author(s):  
Dennis J. Pierro ◽  
Ma Isabel Salazar ◽  
Barry J. Beaty ◽  
Ken E. Olson
Keyword(s):  
Cell Culture ◽  
Dengue Virus ◽  
Virus Type ◽  
Mammalian Cells ◽  
Infectious Clone ◽  
Cell Types ◽  
Dengue Virus Type 2

A full-length infectious cDNA clone (ic) was constructed from the genome of the dengue virus type 2 (DENV-2) Jamaica83 1409 strain, pBAC1409ic, by using a bacterial artifical chromosome plasmid system. Infectious virus was generated and characterized for growth in cell culture and for infection in Aedes aegypti mosquitoes. During construction, an isoleucine to methionine (Ile→Met) change was found at position 6 in the envelope glycoprotein sequence between low- and high-passage DENV-2 1409 strains. In vitro-transcribed genomic RNA of 1409ic with E6-Ile produced infectious virions following electroporation in mosquito cells, but not mammalian cells, while 1409ic RNA with an E6-Met mutation produced virus in both cell types. Moreover, DENV-2 1409 with the E6-Ile residue produced syncytia in C6/36 cell culture, whereas viruses with E6-Met did not. However, in vitro cell culture-derived growth-curve data and in vivo mosquito-infection rates revealed that none of the analysed DENV-2 strains differed from each other.

scholarly journals Chloroquine Inhibits Dengue Virus Type 2 Replication in Vero Cells but Not in C6/36 Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Kleber Juvenal Silva Farias ◽  
Paula Renata Lima Machado ◽  
Benedito Antônio Lopes da Fonseca
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Mammalian Cells ◽  
Plaque Assay ◽  
Antiviral Drug ◽  
Vero Cells ◽  
Significant Difference ◽  
Dengue Virus Type 2

Dengue viruses are the most important arthropod-borne viruses in terms of morbidity and mortality in the world. Since there is no dengue vaccine available for human use, we have set out to investigate the use of chloroquine as an antiviral drug against dengue. Chloroquine, an amine acidotropic drug known to affect intracellular exocytic pathways by increasing endosomal pH, was used in the in vitro treatment of Vero and C6/36 cells infected with dengue virus type 2 (DENV-2). Real-time RT-PCR and plaque assays were used to quantify the DENV-2 load in infected Vero and C6/36 cells after chloroquine treatment. Our results showed that a dose of 50 μg/ml of chloroquine was not toxic to the cells and induced a statistically significant inhibition of virus production in infected Vero cells when compared to untreated cells. In C6/36 cells, chloroquine does not induce a statistically significant difference in viral replication when compared to untreated cells, showing that this virus uses an unlikely pathway of penetration in these cells, and results were also confirmed by the plaque assay (PFU). These data suggest that the inhibition of virus infection induced by chloroquine is due to interference with acidic vesicles in mammalian cells.

Inhibitory effects of curcumin on dengue virus type 2-infected cells in vitro

2013 ◽  
Vol 159 (3) ◽  
pp. 573-579 ◽  
Author(s):  
Leonardo Padilla-S ◽  
Andrés Rodríguez ◽  
María M. Gonzales ◽  
Juan C. Gallego-G ◽  
Jhon C. Castaño-O
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Inhibitory Effects ◽  
Infected Cells ◽  
Dengue Virus Type 2

scholarly journals Replication-Defective Adenoviral Vaccine Vector for the Induction of Immune Responses to Dengue Virus Type 2

2003 ◽  
Vol 77 (23) ◽  
pp. 12907-12913 ◽  
Author(s):  
Smita Jaiswal ◽  
Navin Khanna ◽  
S. Swaminathan
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Neutralizing Antibodies ◽  
Adenovirus Vector ◽  
Interleukin 4 ◽  
Promising Alternative ◽  
Infected Cells ◽  
Dengue Virus Type 2

ABSTRACT A recombinant replication-defective adenovirus vector that can overexpress the ectodomain of the envelope protein of dengue virus type 2 (NGC strain) has been constructed. This virus was immunogenic in mice and elicited dengue virus type 2 specific B- and T-cell responses. Sera from immunized mice contained neutralizing antibodies that could specifically recognize dengue virus type 2 and neutralize its infectivity in vitro, indicating that this approach has the potential to confer protective immunity. In vitro stimulation of splenocytes (from immunized mice) with dengue virus type 2 resulted in a significant proliferative response accompanied by the production of high levels of gamma interferon but did not show significant changes in interleukin-4 levels. This is suggestive of a Th1-like response (considered to be important in the maturation of cytotoxic T lymphocytes that are essential for the elimination of virus-infected cells). The data show that adenovirus vectors offer a promising alternative strategy for the development of dengue virus vaccines.

scholarly journals Novel Antiviral Investigation of Annona squamosa Leaf Extract against the Dengue Virus Type-2: In vitro Study

2020 ◽  
Vol 13 (2) ◽  
pp. 456-462
Author(s):  
Arif Nur Muhammad Ansori ◽  
Amaq Fadholly ◽  
Annise Proboningrat ◽  
Yulanda Antonius ◽  
Suhailah Hayaza ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Leaf Extract ◽  
In Vitro Study ◽  
Vitro Study ◽  
Annona Squamosa ◽  
Dengue Virus Type 2
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