scholarly journals Tomography of the Solar Corona. II. Robust, Regularized, Positive Estimation of the Three‐dimensional Electron Density Distribution from LASCO‐C2 Polarized White‐Light Images

2002 ◽  
Vol 570 (1) ◽  
pp. 408-422 ◽  
Author(s):  
Richard A. Frazin ◽  
Paul Janzen



2014 ◽  
Vol 70 (a1) ◽  
pp. C1752-C1752
Author(s):  
Rino Saiga ◽  
Susumu Takekoshi ◽  
Naoya Nakamura ◽  
Akihisa Takeuchi ◽  
Kentaro Uesugi ◽  
...  

In macromolecular crystallography, an electron density distribution is traced to build a model of the target molecule. We applied this method to model building for electron density maps of a brain network. Human cerebral tissue was stained with heavy atoms [1]. The sample was then analyzed at the BL20XU beamline of SPring-8 to obtain a three-dimensional map of X-ray attenuation coefficients representing the electron density distribution. Skeletonized wire models were built by placing and connecting nodes in the map [2], as shown in the figure below. The model-building procedures were similar to those reported for crystallographic analyses of macromolecular structures, while the neuronal network was automatically traced by using a Sobel filter. Neuronal circuits were then analytically resolved from the skeletonized models. We suggest that X-ray microtomography along with model building in the electron density map has potential as a method for understanding three-dimensional microstructures relevant to biological functions.





Optik ◽  
2021 ◽  
pp. 168169
Author(s):  
S. Saravanakumar ◽  
D. Sivaganesh ◽  
V. Sivakumar ◽  
Yang Li ◽  
Rajajeyaganthan Ramanthan ◽  
...  


Measurements were made during 1948-9 of all the intensities of hkl reflexions observable with chromium Koc X-radiation from air-dried crystals of vitamin B 12 . Calculations parallel with those of J. G. White were carried out on these data. The positions of the cobalt atoms in the crystal structure were found from three-dimensional Patterson series and the positions of 90 atoms of the B 12 molecule and 7 water molecules were derived through three successive approximations to the three-dimensional electron density distribution. The choice of atomic positions was checked against superposition maps derived from the original Patterson series, and assisted by comparisons with other B 12 derivatives. Minor differences appear between the positions derived here and in III; some of these may be real differences due to the state of dryness of the crystals.





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