Microscopic Observation Drug Susceptibility Assay for Tuberculosis Screening before Isoniazid Preventive Therapy in HIV‐Infected Persons

Abstract: Tuberculosis (TB) is a contagious infectious disease caused by Mycobacterium tuberculosis. 10 million people suffer from TB Every year. Although TB is a preventable and treatable disease, 1.5 million people die every year due to TB. Alternative treatments continue to be pursued, and treatment with the latest TB drugs that are continuously being encouraged. Black cumin (Nigella sativa) seed contains essential oils with active compounds such as thymohydroquinone, Oleoresins, flavonoids, alkaloids, saponins, tannins, and terpenoids that act as antibacterial drugs. This study aims to determine the sensitivity of N. sativa seed extract in inhibiting the growth of M. tuberculosis strain H37RV and MDR-TB (Multidrug Resistance-TB). This research using Microscopic-Observation and Drug-Susceptibility Assay (MODS) method. Extraction of N. sativa was carried out by the maceration method using 70% methanol as a solvent. The results showed that the M. tuberculosis strain H37RV and MDR-TB were sensitive to N. sativa extract at concentrations of 5 and 10% but resistant to N. sativa extract at concentrations of 1 and 3%.Abstrak: Tuberkulosis (TB) adalah penyakit menular yang disebabkan oleh Bakteri Mycobacterium tuberculosis. Penyakit ini menimbulkan dampak kematian yang cukup mengkhawatirkan. Penyakit tersebut dapat dicegah dan diobati. Salah satu sumber pengobatannya menggunakan biji jintan hitam (Nigella sativa) yang mengandung minyak atsiri dengan senyawa aktif seperti timohidrokuinon, oleoresin, flavonoid, alkaloid, saponin, tanin, dan terpenoid yang berfungsi sebagai obat antibakteri. Penelitian ini bertujuan untuk mengetahui sensitivitas ekstrak biji N. sativa dalam menghambat pertumbuhan M. tuberculosis strain H37RV and MDR-TB (Multidrug-Resistance-TB). Penelitian ini menggunakan metode Microscopic-Observation and Drug-Susceptibility Assay (MODS). Ekstraksi N. sativa dilakukan dengan metode maserasi menggunakan pelarut metanol 70%. Hasil yang diperoleh menunjukkan bahwa bakteri M. tuberculosis strain H37RV dan TB-MDR, kedua strain tsb sensitif terhadap ekstrak N. sativa konsentrasi 5 dan 10%, tetapi resisten terhadap ekstrak N. sativa konsentrasi 1 dan 3%.

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Evaluation of the microscopic observation drug susceptibility assay for the detection of first- and second-line drug susceptibility forMycobacterium tuberculosis

European Respiratory Journal ◽

10.1183/13993003.02215-2016 ◽

2017 ◽

Vol 49 (4) ◽

pp. 1602215 ◽

Cited By ~ 1

Author(s):

Donald G. Catanzaro ◽

Andre P. Trollip ◽

Marva Seifert ◽

Sophia B. Georghiou ◽

Richard S. Garfein ◽

...

Keyword(s):

Microscopic Observation ◽

Drug Susceptibility ◽

Second Line ◽

Line Drug ◽

Susceptibility Assay

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Improving tuberculosis screening and isoniazid preventive therapy in an HIV clinic in Addis Ababa, Ethiopia

The International Journal of Tuberculosis and Lung Disease ◽

10.5588/ijtld.13.0315 ◽

2013 ◽

Vol 17 (11) ◽

pp. 1396-1401 ◽

Cited By ~ 11

Author(s):

S. Zaeh ◽

R. Kempker ◽

E. Stenehjem ◽

H. M. Blumberg ◽

O. Temesgen ◽

...

Keyword(s):

Addis Ababa ◽

Preventive Therapy ◽

Isoniazid Preventive Therapy ◽

Tuberculosis Screening

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Evaluation of nitrate reduction assay, resazurin microtiter assay and microscopic observation drug susceptibility assay for first line antitubercular drug susceptibility testing of clinical isolates of M. tuberculosis

Journal of Microbiological Methods ◽

10.1016/j.mimet.2011.11.006 ◽

2012 ◽

Vol 88 (1) ◽

pp. 122-126 ◽

Cited By ~ 23

Author(s):

Pratima Dixit ◽

Urmila Singh ◽

Pratibha Sharma ◽

Amita Jain

Keyword(s):

Nitrate Reduction ◽

Microscopic Observation ◽

Susceptibility Testing ◽

Drug Susceptibility ◽

Drug Susceptibility Testing ◽

Antitubercular Drug ◽

First Line ◽

Reduction Assay ◽

Microtiter Assay ◽

Susceptibility Assay

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Tuberculosis screening coverage and isoniazid preventive therapy among people living with HIV at Gambella Hospital, southwest Ethiopia: a retrospective study design

Journal of International Medical Research ◽

10.1177/03000605211046133 ◽

2021 ◽

Vol 49 (9) ◽

pp. 030006052110461

Author(s):

Mecha Aboma ◽

Nagasa Dida

Keyword(s):

Retrospective Study ◽

Complete Information ◽

Preventive Therapy ◽

Isoniazid Preventive Therapy ◽

World Health ◽

People Living With Hiv ◽

African Countries ◽

Tuberculosis Screening ◽

Southwest Ethiopia ◽

Health Organization

Objective We aimed to assess the coverage of tuberculosis screening and isoniazid preventive therapy (IPT) among people living with human immunodeficiency virus (PLHIV) at Gambella Hospital, southwest Ethiopia. Methods We conducted a 5-year retrospective study of PLHIV receiving care in an antiretroviral therapy clinic from 1 January 2011 to 30 December 2015. We reviewed a total of 900 medical records of patients with complete information. Result Of the total, 897 (99.7%) PLHIV were screened for tuberculosis, among which 77 (8.6%) were found to be positive for active tuberculosis. Among 820 (91.4%) individuals eligible for IPT, only 545 (66.5%) were provided IPT; 275 (33.5%) eligible PLHIV were not provided IPT. Male sex (adjusted odds ratio [AOR] 1.63) and ages 18–29 years (AOR 0.33) and 30–44 years (AOR 0.31) were significantly associated with the likelihood of tuberculosis infection. Conclusion The present study findings demonstrated that tuberculosis screening for PLHIV at Gambella Hospital was improved in comparison with reports from many African countries and other parts of Ethiopia. Despite this improvement, the implementation rate of IPT was below national and World Health Organization recommendations. Overall, tuberculosis diagnostic approaches and available preventive measures should be strengthened in the study area.

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