scholarly journals Black Cumin (Nigella sativa) Against Mycobacterium tuberculosis Strain H37RV And MDR-TB

Elkawnie ◽  
2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Mashuri Masri ◽  
Cut Muthiadin ◽  
Masita Masita ◽  
Tri Cahyanto ◽  
Lianah Lianah ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Multidrug Resistance ◽  
Microscopic Observation ◽  
Nigella Sativa ◽  
Drug Susceptibility ◽  
Tuberculosis Strain ◽  
Black Cumin ◽  
Mdr Tb ◽  
Susceptibility Assay ◽  
Strain H37rv

Abstract: Tuberculosis (TB) is a contagious infectious disease caused by Mycobacterium tuberculosis. 10 million people suffer from TB Every year. Although TB is a preventable and treatable disease, 1.5 million people die every year due to TB. Alternative treatments continue to be pursued, and treatment with the latest TB drugs that are continuously being encouraged. Black cumin (Nigella sativa) seed contains essential oils with active compounds such as thymohydroquinone, Oleoresins, flavonoids, alkaloids, saponins, tannins, and terpenoids that act as antibacterial drugs. This study aims to determine the sensitivity of  N. sativa seed extract in inhibiting the growth of  M. tuberculosis strain H37RV and MDR-TB (Multidrug Resistance-TB). This research using Microscopic-Observation and Drug-Susceptibility Assay (MODS) method. Extraction of N. sativa was carried out by the maceration method using 70% methanol as a solvent. The results showed that the M. tuberculosis strain H37RV and MDR-TB were sensitive to N. sativa extract at concentrations of 5 and 10% but resistant to N. sativa extract at concentrations of 1 and 3%.Abstrak: Tuberkulosis (TB) adalah penyakit menular yang disebabkan oleh Bakteri Mycobacterium tuberculosis. Penyakit ini menimbulkan dampak kematian yang cukup mengkhawatirkan.  Penyakit tersebut dapat dicegah dan diobati. Salah satu sumber pengobatannya menggunakan biji jintan hitam (Nigella sativa) yang mengandung minyak atsiri dengan senyawa aktif seperti timohidrokuinon, oleoresin, flavonoid, alkaloid, saponin, tanin, dan terpenoid yang berfungsi sebagai obat antibakteri. Penelitian ini bertujuan untuk mengetahui sensitivitas ekstrak biji N. sativa dalam menghambat pertumbuhan M. tuberculosis strain H37RV and MDR-TB (Multidrug-Resistance-TB). Penelitian ini menggunakan metode Microscopic-Observation and Drug-Susceptibility Assay (MODS). Ekstraksi N. sativa dilakukan dengan metode maserasi menggunakan pelarut metanol 70%. Hasil yang diperoleh menunjukkan bahwa bakteri M. tuberculosis strain H37RV dan TB-MDR, kedua  strain tsb sensitif terhadap ekstrak N. sativa konsentrasi 5 dan 10%,  tetapi resisten terhadap  ekstrak N. sativa konsentrasi 1 dan 3%.

scholarly journals Aktivitas Anti Mycobacterium tuberculosis Strain H37Rv dan MDR (Multi Drug Resistant) dari Ekstrak Rimpang Curcuma mangga Val.

2020 ◽  
Vol 6 (1) ◽  
pp. 70-76
Author(s):  
Ritha Pratiwi ◽  
Gemini Alam ◽  
Herlina Rante ◽  
Nasrum Massi
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Microscopic Observation ◽  
Methanol Extract ◽  
Hexane Extract ◽  
Tuberculosis Strain ◽  
Tuberculosis Activity ◽  
Strain H37rv ◽  
Mycobacterium Tuberculosis Strain ◽  
Made In

TB is an infectious disease caused by the bacterium Mycobacterium tuberculosis. Empirically the plants that have long been used in medicine are Curcuma mangga  rhizome, which shows pharmacological activity as an anti Mycobacterium tuberculosis drug. The objective of the research is to determine the ability of hexane and methanol extracts of Curcuma mangga in inhibiting the growth of Mycobacterium tuberculosis Strain H37Rv and MDR. The extraction of Curcuma mangga rhizome was conducted by maceration methode using hexane and methanol as the solvents, respectively.  Both of extracts were made in 1000 dan 500 ppm concentration, then tested for anti Mycobacterium tuberculosis activity using MODS (Microscopic Observation Drug Suspectibility) method. Determination of anti M.tuberculosis activity was based on microscopic observation on Mycobacterium tuberculosis colony in cord formation. The results showed that hexane and methanol extract in 1000 ppm concentration inhibited the growth of Mycobacterium tuberculosis Strain H37Rv dan MDR. However in 500 ppm only n-hexane  extract showing the inhibiton activity. In conclusion, n-hexane extract have the ability to inhibit the growth of Mycobacterium tuberculosis Strain H37Rv dan MDR. 

Rapid Diagnosis of Tuberculosis and Multidrug Resistance by the Microscopic-Observation Drug-Susceptibility Assay

2011 ◽  
Vol 183 (10) ◽  
pp. 1427-1433 ◽  
Author(s):  
N. Sarita Shah ◽  
Prashini Moodley ◽  
Palav Babaria ◽  
Salona Moodley ◽  
Melissa Ramtahal ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Microscopic Observation ◽  
Drug Susceptibility ◽  
Rapid Diagnosis ◽  
Susceptibility Assay

scholarly journals Evaluation of Microscopic Observation Drug Susceptibility Assay for Detection of Multidrug-Resistant Mycobacterium tuberculosis

2007 ◽  
Vol 45 (4) ◽  
pp. 1093-1097 ◽  
Author(s):  
G. Shiferaw ◽  
Y. Woldeamanuel ◽  
M. Gebeyehu ◽  
F. Girmachew ◽  
D. Demessie ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Microscopic Observation ◽  
Drug Susceptibility ◽  
Multidrug Resistant ◽  
Susceptibility Assay

scholarly journals Evaluation of the microscopic observation drug susceptibility assay for detection of Mycobacterium tuberculosis resistance to pyrazinamide

2011 ◽  
Vol 17 (12) ◽  
pp. 1792-1797 ◽  
Author(s):  
L.D. Ghiraldi ◽  
P.A.Z. Campanerut ◽  
F.L.E. Spositto ◽  
D.N. Sato ◽  
C.Q.F. Leite ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Microscopic Observation ◽  
Drug Susceptibility ◽  
Susceptibility Assay

scholarly journals Rapid Identification and Drug Susceptibility Testing of Mycobacterium tuberculosis: Standard Operating Procedure for Non-Commercial Assays: Part 1: Microscopic Observation Drug Susceptibility Assay v2.4.12

2012 ◽  
Vol 4 (02) ◽  
pp. 101-111 ◽  
Author(s):  
Sarman Singh ◽  
Parveen Kumar ◽  
Shreya Sharma ◽  
Francis Mumbowa ◽  
Anandi Martin ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Operating Procedure ◽  
Microscopic Observation ◽  
Susceptibility Testing ◽  
Standard Operating Procedure ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Health Concern ◽  
Standard Operating ◽  
Susceptibility Assay

ABSTRACTMultidrug-resistant tuberculosis is an increasing public health concern in many parts of the world, especially in low-income countries, where most cases occur. Traditional mycobacteria culture and drug susceptibility testing (DST) is either time-consuming or expensive and for that reason uptake of these technologies has remained limited in many resource-constrained settings. However, several non-commercial culture and DST methods that do not require sophisticated infrastructure and techniques have been developed. One such method is the microscopic observation drug susceptibility assay (MODS). In this method microcolonies that form in the liquid culture medium after specimen inoculation to drug-free and drug-containing micro-wells are detected by visual observation with a simple inverted microscope. The identification and drug susceptibility results can be obtained in 7-15 days. This standard operating procedure document has been developed through the culture and DST subgroup of the STOP TB Partnership, New Diagnostic Working Group. It is intended for laboratories that would want to use or already using this rapid non-commercial method for culture identification and DST of Mycobacterium tuberculosis, notably in resource-constraint settings in Asia and Africa.

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