Evaluation of nitrate reduction assay, resazurin microtiter assay and microscopic observation drug susceptibility assay for first line antitubercular drug susceptibility testing of clinical isolates of M. tuberculosis

2012 ◽  
Vol 88 (1) ◽  
pp. 122-126 ◽  
Author(s):  
Pratima Dixit ◽  
Urmila Singh ◽  
Pratibha Sharma ◽  
Amita Jain
Keyword(s):  
Nitrate Reduction ◽  
Microscopic Observation ◽  
Susceptibility Testing ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Antitubercular Drug ◽  
First Line ◽  
Reduction Assay ◽  
Microtiter Assay ◽  
Susceptibility Assay

scholarly journals Microscopic Observation Drug Susceptibility Assay for Rapid Diagnosis of Lymph Node Tuberculosis and Detection of Drug Resistance

2015 ◽  
Vol 54 (1) ◽  
pp. 185-189 ◽  
Author(s):  
Daniela E. Kirwan ◽  
Cesar Ugarte-Gil ◽  
Robert H. Gilman ◽  
Luz Caviedes ◽  
Hasan Rizvi ◽  
...  
Keyword(s):  
Microscopic Observation ◽  
Susceptibility Testing ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Rapid Diagnosis ◽  
Accurate Diagnosis ◽  
Lower Sensitivity ◽  
Resource Poor ◽  
Lymph Node Tuberculosis ◽  
Susceptibility Assay

In this study, 132 patients with lymphadenopathy were investigated. Fifty-two (39.4%) were diagnosed with tuberculosis (TB). The microscopic observation drug susceptibility (MODS) assay provided rapid (13 days), accurate diagnosis (sensitivity, 65.4%) and reliable drug susceptibility testing (DST). Despite its lower sensitivity than that of other methods, its faster results and simultaneous DST are advantageous in resource-poor settings, supporting the incorporation of MODS into diagnostic algorithms for extrapulmonary TB.

scholarly journals Rapid Identification and Drug Susceptibility Testing of Mycobacterium tuberculosis: Standard Operating Procedure for Non-Commercial Assays: Part 1: Microscopic Observation Drug Susceptibility Assay v2.4.12

2012 ◽  
Vol 4 (02) ◽  
pp. 101-111 ◽  
Author(s):  
Sarman Singh ◽  
Parveen Kumar ◽  
Shreya Sharma ◽  
Francis Mumbowa ◽  
Anandi Martin ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Operating Procedure ◽  
Microscopic Observation ◽  
Susceptibility Testing ◽  
Standard Operating Procedure ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Health Concern ◽  
Standard Operating ◽  
Susceptibility Assay

ABSTRACTMultidrug-resistant tuberculosis is an increasing public health concern in many parts of the world, especially in low-income countries, where most cases occur. Traditional mycobacteria culture and drug susceptibility testing (DST) is either time-consuming or expensive and for that reason uptake of these technologies has remained limited in many resource-constrained settings. However, several non-commercial culture and DST methods that do not require sophisticated infrastructure and techniques have been developed. One such method is the microscopic observation drug susceptibility assay (MODS). In this method microcolonies that form in the liquid culture medium after specimen inoculation to drug-free and drug-containing micro-wells are detected by visual observation with a simple inverted microscope. The identification and drug susceptibility results can be obtained in 7-15 days. This standard operating procedure document has been developed through the culture and DST subgroup of the STOP TB Partnership, New Diagnostic Working Group. It is intended for laboratories that would want to use or already using this rapid non-commercial method for culture identification and DST of Mycobacterium tuberculosis, notably in resource-constraint settings in Asia and Africa.

scholarly journals Comparison of the Performances of Two In-House Rapid Methods for Antitubercular Drug Susceptibility Testing

2008 ◽  
Vol 53 (2) ◽  
pp. 808-810 ◽  
Author(s):  
Agustina I. de la Iglesia ◽  
Emma J. Stella ◽  
Héctor R. Morbidoni
Keyword(s):  
Drug Resistance ◽  
Mycobacterium Tuberculosis ◽  
Sensitivity And Specificity ◽  
Susceptibility Testing ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Antitubercular Drug ◽  
Rapid Methods ◽  
Resistance Assessment

ABSTRACT Resistance to rifampin (rifampicin), isoniazid, and streptomycin of 69 Mycobacterium tuberculosis isolates was analyzed by an in-house method based on mycobacteriophage D29 and a colorimetric micromethod. Both methods showed sensitivity and specificity values ranging from 93% to 100%. These simple methods offer an option for drug resistance assessment of M. tuberculosis.

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