Pre-polarization enhancement by dynamic nuclear polarization in SQUID-based ultra-low-field nuclear magnetic resonance

2010 ◽  
Vol 23 (11) ◽  
pp. 115008 ◽  
Author(s):  
Seong-Joo Lee ◽  
Kiwoong Kim ◽  
Chan Seok Kang ◽  
Seong-min Hwang ◽  
Yong-Ho Lee
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization ◽  
Low Field ◽  
Nuclear Magnetic

The effect of glassing solvent deuteration and Gd3+ doping on 13C DNP at 5 T

RSC Advances ◽  
2016 ◽  
Vol 6 (45) ◽  
pp. 38855-38860 ◽  
Author(s):  
Andhika Kiswandhi ◽  
Bimala Lama ◽  
Peter Niedbalski ◽  
Mudrekh Goderya ◽  
Joanna Long ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Dynamic Nuclear Polarization ◽  
Sodium Acetate ◽  
Nuclear Polarization ◽  
Nuclear Magnetic

We report the influence of glassing solvent deuteration and Gd3+ doping on 13C dynamic nuclear polarization (DNP) nuclear magnetic resonance (NMR) performed on [1-13C] sodium acetate at B0 = 5 T and 1.2 K.

scholarly journals Competing transfer pathways in direct and indirect dynamic nuclear polarization magic anglespinning nuclear magnetic resonance experiments on HIV-1 capsid assemblies: implications for sensitivity and resolution

2021 ◽  
Vol 2 (1) ◽  
pp. 239-249
Author(s):  
Ivan V. Sergeyev ◽  
Caitlin M. Quinn ◽  
Jochem Struppe ◽  
Angela M. Gronenborn ◽  
Tatyana Polenova
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization ◽  
Magic Angle Spinning ◽  
Magic Angle ◽  
Large Signal ◽  
Experimental Conditions ◽  
Hiv 1 ◽  
Nuclear Magnetic

Abstract. Dynamic nuclear polarization (DNP)-enhanced magic angle spinning (MAS) nuclear magnetic resonance (NMR) of biological systems is a rapidly growing field. Large signal enhancements make the technique particularly attractive for signal-limited cases, such as studies of complex biological assemblies or at natural isotopic abundance. However, spectral resolution is considerably reduced compared to ambient-temperature non-DNP spectra. Herein, we report a systematic investigation into sensitivity and resolution of 1D and 2D 13C-detected DNP MAS NMR experiments on HIV-1 CA capsid protein tubular assemblies. We show that the magnitude and sign of signal enhancement as well as the homogeneous line width are strongly dependent on the biradical concentration, the dominant polarization transfer pathway, and the enhancement buildup time. Our findings provide guidance for optimal choice of sample preparation and experimental conditions in DNP experiments.

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