scholarly journals DNA-barcoding as molecular marker for seafood forensics: Species identification of locally consumed shark fish products in the world’s largest shark fishery

Author(s):  
E Muttaqin ◽  
A Abdullah ◽  
M Nurilmala ◽  
M Ichsan ◽  
B M Simeone ◽  
...  
2014 ◽  
Vol 24 (2) ◽  
pp. 119-127 ◽  
Author(s):  
Fangping CHENG ◽  
Minxiao WANG ◽  
Song SUN ◽  
Chaolun LI ◽  
Yongshan ZHANG

2016 ◽  
Vol 202 ◽  
pp. 116-119 ◽  
Author(s):  
Shuai Yan ◽  
Guiyan Lai ◽  
Li Li ◽  
Hao Xiao ◽  
Ming Zhao ◽  
...  

Mammalia ◽  
2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Álvaro J. Benítez ◽  
Dina Ricardo-Caldera ◽  
María Atencia-Pineda ◽  
Jesús Ballesteros-Correa ◽  
Julio Chacón-Pacheco ◽  
...  

Abstract Bats are mammals of great ecological and medical importance, which have associations with different pathogenic microorganisms. DNA barcoding is a tool that can expedite species identification using short DNA sequences. In this study, we assess the DNA barcoding methodology in bats from the Colombian Northern region, specifically in the Córdoba department. Cytochrome oxidase subunit I (COI) gene sequences of nine bat species were typified, and their comparison with other Neotropic samples revealed that this marker is suitable for individual species identification, with ranges of intra-species variation from 0.1 to 0.9%. Bat species clusters are well supported and differentiated, showing average genetic distances ranging from 3% between Artibeus lituratus and Artibeus planirostris, up to 27% between Carollia castanea and Molossus molossus. C. castanea and Glossophaga soricina show geographical structuring in the Neotropic. The findings reported in this study confirm DNA barcoding usefulness for fast species identification of bats in the region.


Genome ◽  
2006 ◽  
Vol 49 (7) ◽  
pp. 851-854 ◽  
Author(s):  
Mehrdad Hajibabaei ◽  
Gregory AC Singer ◽  
Donal A Hickey

DNA barcoding has been recently promoted as a method for both assigning specimens to known species and for discovering new and cryptic species. Here we test both the potential and the limitations of DNA barcodes by analysing a group of well-studied organisms—the primates. Our results show that DNA barcodes provide enough information to efficiently identify and delineate primate species, but that they cannot reliably uncover many of the deeper phylogenetic relationships. Our conclusion is that these short DNA sequences do not contain enough information to build reliable molecular phylogenies or define new species, but that they can provide efficient sequence tags for assigning unknown specimens to known species. As such, DNA barcoding provides enormous potential for use in global biodiversity studies.Key words: DNA barcoding, species identification, primate, biodiversity.


Author(s):  
Qian Tang ◽  
Qi Luo ◽  
Qian Duan ◽  
Lei Deng ◽  
Renyi Zhang

Nowadays, the global fish consumption continues to rise along with the continuous growth of the population, which has led to the dilemma of overfishing of fishery resources. Especially high-value fish that are overfished are often replaced by other fish. Therefore, the accurate identification of fish products in the market is a problem worthy of attention. In this study, full-DNA barcoding (FDB) and mini-DNA barcoding (MDB) used to detect the fraud of fish products in Guiyang, Guizhou province in China. The molecular identification results showed that 39 of the 191 samples were not consistent with the labels. The mislabelling of fish products for fresh, frozen, cooked and canned were 11.70%, 20.00%, 34.09% and 50.00%, respectively. The average kimura 2 parameter distances of MDB within species and genera were 0.27% and 5.41%, respectively; while average distances of FDB were 0.17% within species and 6.17% within genera. In this study, commercial fraud is noticeable, most of the high-priced fish were replaced of low-priced fish with a similar feature. Our study indicated that DNA barcoding is a valid tool for the identification of fish products and that it allows an idea of conservation and monitoring efforts, while confirming the MDB as a reliable tool for fish products.


2019 ◽  
Vol 3 (2) ◽  
pp. 28-35
Author(s):  
Nurlisa A Butet ◽  
Inge Anggraeni Bela Putri Dewi ◽  
Zairion Zairion ◽  
Agus Alim Hakim

Undur-undur laut hidup pada habitat intertidal pantai berpasir. Identifikasi spesies akuatik sering mengalami kesalahan yang diakibatkan oleh fenomena cryptic spesies, sehingga diperlukan teknik identifikasi dengan pendekatan molekuler yaitu DNA barcoding. Penelitian ini bertujuan untuk menvalidasi dan menganalisis hubungan kekerabatan dari undur-undur laut berdasarkan marka molekuler gen 16S rRNA dari perairan Bantul dan Purworejo. Kit komersil berupa Gene Aid digunakan untuk isolasi dan ekstraksi DNA dan dihasilkan tiga DNA total dari setiap lokasi. DNA total dengan kualitas baik dilanjutkan pada proses amplifikasi menggunakan teknik PCR dengan primer 16S rRNA. Hasil sekuen gen 16S rRNA sampel undur-undur laut disejajarkan dengan beberapa sekuen dengan spesies lain dari genus Emerita, Scylla, dan Portunus yang berasal dari GeneBank. Validasi spesies dilakukan dengan mengunakan BLASTn. Jarak genetik dan pohon filogenik didapatkan dari program MEGA 5.0. Sampel undur-undur laut yang berasal dari Bantul dan Purworejo tidak berbeda nyata dan sampel berasal dari sumber genetik yang sama.


2019 ◽  
Vol 49 (8) ◽  
pp. 1032-1040
Author(s):  
LiQiu ZHANG ◽  
JingYuan SONG ◽  
CaiXiang XIE ◽  
JinXin LIU ◽  
Li XIANG ◽  
...  

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