DNA barcoding reveals mislabeling of imported fish products in Nansha new port of Guangzhou, Guangdong province, China

Nowadays, the global fish consumption continues to rise along with the continuous growth of the population, which has led to the dilemma of overfishing of fishery resources. Especially high-value fish that are overfished are often replaced by other fish. Therefore, the accurate identification of fish products in the market is a problem worthy of attention. In this study, full-DNA barcoding (FDB) and mini-DNA barcoding (MDB) used to detect the fraud of fish products in Guiyang, Guizhou province in China. The molecular identification results showed that 39 of the 191 samples were not consistent with the labels. The mislabelling of fish products for fresh, frozen, cooked and canned were 11.70%, 20.00%, 34.09% and 50.00%, respectively. The average kimura 2 parameter distances of MDB within species and genera were 0.27% and 5.41%, respectively; while average distances of FDB were 0.17% within species and 6.17% within genera. In this study, commercial fraud is noticeable, most of the high-priced fish were replaced of low-priced fish with a similar feature. Our study indicated that DNA barcoding is a valid tool for the identification of fish products and that it allows an idea of conservation and monitoring efforts, while confirming the MDB as a reliable tool for fish products.

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Hedyotis nanlingensis (Rubiaceae), a new species from South China

Phytotaxa ◽

10.11646/phytotaxa.206.1.6 ◽

2015 ◽

Vol 206 (1) ◽

pp. 37 ◽

Cited By ~ 1

Author(s):

Ruijiang Wang ◽

Shujun Deng ◽

Xiangxu Huang

Keyword(s):

New Species ◽

Dna Barcoding ◽

South China ◽

Guangdong Province ◽

Monophyletic Lineage ◽

A New Species

A new species, Hedyotis nanlingensis from Guangdong province, China, is described and illustrated. It is morphologically similar to H. nankunshanensis but differs in the smaller and more slender habit, the sessile leaves, the usually two to three secondary veins on each side of the midvein, the inflorescences with monochasial branching and the shorter style in the long-styled flowers. DNA barcoding analysis demonstrates that all accessions from H. nanlingensis form a monophyletic lineage closely related to H. cantoniensis.

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DNA Barcoding Revealed Mislabeling and Potential Health Concerns with Roasted Fish Products Sold across China

Journal of Food Protection ◽

10.4315/0362-028x.jfp-18-514 ◽

2019 ◽

Vol 82 (7) ◽

pp. 1200-1209 ◽

Cited By ~ 7

Author(s):

XIONG XIONG ◽

FANGYING YUAN ◽

MANHONG HUANG ◽

LIXIA LU ◽

XIAOHUI XIONG ◽

...

Keyword(s):

Dna Barcoding ◽

Fish Species ◽

Morphological Characteristics ◽

Economic Loss ◽

High Price ◽

Snack Food ◽

Fish Fillet ◽

Potential Health ◽

Fish Products ◽

Republic Of China

ABSTRACTRoasted fish fillet is a popular snack food in the People's Republic of China, and the market appeal can be reflected by the high price, which exceeds 330 RMB/kg in some cases. However, the labeled fish common name generally cannot be connected with a particular species, and the processing treatments make it a challenging task to identify fish species based on morphological characteristics. This study used full DNA barcoding and mini-DNA barcoding for species identification of 202 specimens sorted from 64 roasted fish fillet products sold across China, which belonged to 32 distinct brands and claimed 16 fish common names on the label. Given the absence of a harmonization in defining these common names in China, scientific literature and the Latin-Chinese dictionary of fish names (http://fishdb.sinica.edu.tw/) were consulted to define at least the family for each common name, and an identified species outside the expected family was determined to be mislabeled. Results highlighted a high mislabeling rate of 75.5%. Even worse, fish species were identified as belonging to multiple families, not only from several products of the same brand under the same common name but also from several specimens sorted from the same product. Moreover, a health issue was highlighted by the identification of toxic Tetraodontidae species from one product. To protect the consumers from economic loss and even health risks, we recommended amendments to existing legislation and the compilation of a list of acceptable market names for fish species in China. Meanwhile, DNA barcoding is a powerful tool in fish forensics, and we recommended the use of this technique to assure the accurate species labeling of fish products.HIGHLIGHTS

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DNA-barcoding as molecular marker for seafood forensics: Species identification of locally consumed shark fish products in the world’s largest shark fishery

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/278/1/012049 ◽

2019 ◽

Vol 278 ◽

pp. 012049

Author(s):

E Muttaqin ◽

A Abdullah ◽

M Nurilmala ◽

M Ichsan ◽

B M Simeone ◽

...

Keyword(s):

Molecular Marker ◽

Dna Barcoding ◽

Species Identification ◽

Fish Products

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DNA Barcoding Analysis and Phylogenetic Relation of Mangroves in Guangdong Province, China

Forests ◽

10.3390/f10010056 ◽

2019 ◽

Vol 10 (1) ◽

pp. 56 ◽

Cited By ~ 5

Author(s):

Feng Wu ◽

Mei Li ◽

Baowen Liao ◽

Xin Shi ◽

Yong Xu

Keyword(s):

Dna Barcoding ◽

Species Identification ◽

Dna Barcode ◽

Phylogenetic Reconstruction ◽

Pcr Amplification ◽

Guangdong Province ◽

Nuclear Ribosomal Dna ◽

Success Rates ◽

Mangrove Species ◽

Identification Rate

Mangroves are distributed in the transition zone between sea and land, mostly in tropical and subtropical areas. They provide important ecosystem services and are therefore economically valuable. DNA barcoding is a useful tool for species identification and phylogenetic reconstruction. To evaluate the effectiveness of DNA barcoding in identifying mangrove species, we sampled 135 individuals representing 23 species, 22 genera, and 17 families from Zhanjiang, Shenzhen, Huizhou, and Shantou in the Guangdong province, China. We tested the universality of four DNA barcodes, namely rbcL, matK, trnH-psbA, and the internal transcribed spacer of nuclear ribosomal DNA (ITS), and examined their efficacy for species identification and the phylogenetic reconstruction of mangroves. The success rates for PCR amplification of rbcL, matK, trnH-psbA, and ITS were 100%, 80.29% ± 8.48%, 99.38% ± 1.25%, and 97.18% ± 3.25%, respectively, and the rates of DNA sequencing were 100%, 75.04% ± 6.26%, 94.57% ± 5.06%, and 83.35% ± 4.05%, respectively. These results suggest that both rbcL and trnH–psbA are universal in mangrove species from the Guangdong province. The highest success rate for species identification was 84.48% ± 12.09% with trnH-psbA, followed by rbcL (82.16% ± 9.68%), ITS (66.48% ± 5.97%), and matK (65.09% ± 6.00%), which increased to 91.25% ± 9.78% with the addition of rbcL. Additionally, the identification rate of mangroves was not significantly different between rbcL + trnH-psbA and other random fragment combinations. In conclusion, rbcL and trnH-psbA were the most suitable DNA barcode fragments for species identification in mangrove plants. When the phylogenetic relationships were constructed with random fragment combinations, the optimal evolutionary tree with high supporting values (86.33% ± 4.16%) was established using the combination of matK + rbcL + trnH-psbA + ITS in mangroves. In total, the 476 newly acquired sequences in this study lay the foundation for a DNA barcode database of mangroves.

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