Herbalogical studies of Ranae oviductus (Hamayou) and its species identification using DNA barcoding method

Abstract Bats are mammals of great ecological and medical importance, which have associations with different pathogenic microorganisms. DNA barcoding is a tool that can expedite species identification using short DNA sequences. In this study, we assess the DNA barcoding methodology in bats from the Colombian Northern region, specifically in the Córdoba department. Cytochrome oxidase subunit I (COI) gene sequences of nine bat species were typified, and their comparison with other Neotropic samples revealed that this marker is suitable for individual species identification, with ranges of intra-species variation from 0.1 to 0.9%. Bat species clusters are well supported and differentiated, showing average genetic distances ranging from 3% between Artibeus lituratus and Artibeus planirostris, up to 27% between Carollia castanea and Molossus molossus. C. castanea and Glossophaga soricina show geographical structuring in the Neotropic. The findings reported in this study confirm DNA barcoding usefulness for fast species identification of bats in the region.

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Benchmarking DNA barcodes: an assessment using available primate sequences

Genome ◽

10.1139/g06-025 ◽

2006 ◽

Vol 49 (7) ◽

pp. 851-854 ◽

Cited By ~ 48

Author(s):

Mehrdad Hajibabaei ◽

Gregory AC Singer ◽

Donal A Hickey

Keyword(s):

New Species ◽

Cryptic Species ◽

Dna Barcoding ◽

Species Identification ◽

Dna Sequences ◽

Phylogenetic Relationships ◽

Primate Species ◽

Dna Barcodes ◽

Global Biodiversity ◽

Efficient Sequence

DNA barcoding has been recently promoted as a method for both assigning specimens to known species and for discovering new and cryptic species. Here we test both the potential and the limitations of DNA barcodes by analysing a group of well-studied organisms—the primates. Our results show that DNA barcodes provide enough information to efficiently identify and delineate primate species, but that they cannot reliably uncover many of the deeper phylogenetic relationships. Our conclusion is that these short DNA sequences do not contain enough information to build reliable molecular phylogenies or define new species, but that they can provide efficient sequence tags for assigning unknown specimens to known species. As such, DNA barcoding provides enormous potential for use in global biodiversity studies.Key words: DNA barcoding, species identification, primate, biodiversity.

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DNA BARCODING: FAST-TRACKING SPECIES IDENTIFICATION

Biodiversity ◽

10.1080/14888386.2006.9712813 ◽

2006 ◽

Vol 7 (3-4) ◽

pp. 71-79

Author(s):

Stephen Aitken

Keyword(s):

Dna Barcoding ◽

Species Identification ◽

Fast Tracking

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Assessing universality of DNA barcoding in geographically isolated selected desert medicinal species of Fabaceae and Poaceae

PeerJ ◽

10.7717/peerj.4499 ◽

2018 ◽

Vol 6 ◽

pp. e4499 ◽

Cited By ~ 1

Author(s):

Aisha Tahir ◽

Fatma Hussain ◽

Nisar Ahmed ◽

Abdolbaset Ghorbani ◽

Amer Jamil

Keyword(s):

Dna Barcoding ◽

Species Identification ◽

Phylogenetic Trees ◽

Sequence Similarity ◽

Sequence Divergence ◽

Species Level ◽

Practical Implementation ◽

Medicinal Species ◽

Level Identification ◽

Geographically Isolated

In pursuit of developing fast and accurate species-level molecular identification methods, we tested six DNA barcodes, namely ITS2, matK, rbcLa, ITS2+matK, ITS2+rbcLa, matK+rbcLa and ITS2+matK+rbcLa, for their capacity to identify frequently consumed but geographically isolated medicinal species of Fabaceae and Poaceae indigenous to the desert of Cholistan. Data were analysed by BLASTn sequence similarity, pairwise sequence divergence in TAXONDNA, and phylogenetic (neighbour-joining and maximum-likelihood trees) methods. Comparison of six barcode regions showed that ITS2 has the highest number of variable sites (209/360) for tested Fabaceae and (106/365) Poaceae species, the highest species-level identification (40%) in BLASTn procedure, distinct DNA barcoding gap, 100% correct species identification in BM and BCM functions of TAXONDNA, and clear cladding pattern with high nodal support in phylogenetic trees in both families. ITS2+matK+rbcLa followed ITS2 in its species-level identification capacity. The study was concluded with advocating the DNA barcoding as an effective tool for species identification and ITS2 as the best barcode region in identifying medicinal species of Fabaceae and Poaceae. Current research has practical implementation potential in the fields of pharmaco-vigilance, trade of medicinal plants and biodiversity conservation.

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Preliminary study: Species identification of Rasbora sumatrana through the cytochrome oxidase subunit I DNA barcoding marker

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/674/1/012107 ◽

2021 ◽

Vol 674 (1) ◽

pp. 012107

Author(s):

M Y Norfatimah ◽

M A R Akram ◽

W Z A Lyena ◽

Z N Aili ◽

A Farizan

Keyword(s):

Cytochrome Oxidase ◽

Dna Barcoding ◽

Species Identification ◽

Cytochrome Oxidase Subunit ◽

Cytochrome Oxidase Subunit I ◽

Oxidase Subunit ◽

Preliminary Study ◽

Study Species

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DNA Barcoding on Bacteria: A Review

Advances in Biology ◽

10.1155/2014/541787 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 13

Author(s):

D. E. Lebonah ◽

A. Dileep ◽

K. Chandrasekhar ◽

S. Sreevani ◽

B. Sreedevi ◽

...

Keyword(s):

Dna Barcoding ◽

Species Identification ◽

Agricultural Production ◽

Sequence Data ◽

Bacterial Pathogens ◽

Review Article ◽

Food Spoilage ◽

Identification Technique

Bacteria are omnipotent and they can be found everywhere. The study of bacterial pathogens has been happening from olden days to prevent epidemics, food spoilage, losses in agricultural production, and loss of lives. Modern techniques in DNA based species identification are considered. So, there is a need to acquire simple and quick identification technique. Hence, this review article covers the efficacy of DNA barcoding of bacteria. Routine DNA barcoding involves the production of PCR amplicons from particular regions to sequence them and these sequence data are used to identify or “barcode” that organism to make a distinction from other species.

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Use of molecular methods in identification of species, age and sex of insects useful in forensic entomology

Issues of Forensic Science ◽

10.34836/pk.2014.286.4 ◽

2014 ◽

Vol 286 ◽

pp. 66-69

Author(s):

Joanna Stojak ◽

Keyword(s):

Dna Barcoding ◽

Species Identification ◽

Environmental Conditions ◽

Forensic Entomology ◽

Sex Identification ◽

The Body ◽

Molecular Methods ◽

Insect Larvae ◽

Identification Of Species ◽

Age And Sex

Forensic entomology uses insects to determine the time, cause and place of death. To this end, two entomological methods are used. The development-based method uses the patterns of insect larvae development under the specific thermal and environmental conditions. The succession-based method analyzes the sequence of insect succession on the body in various environmental conditions. The proper insect species identification is essential in both methods. In this article, the molecular methods of species, age and sex identification are presented such as DNA barcoding or DNA-HRM-PCR.

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