The effect of genotype on in vitro morphogenesis of blue- and purple-grained bread wheat

Aim. Receiving of genetically modified plants of bread wheat with heterologous ornithine‑δ‑aminotransferase gene. Methods. Agrobacterium-mediated transformation of callus cultures in vitro, PCR-analysis. Results. By Agrobacterium-mediated transformation of the morphogenic calluses of bread wheat (Triticum aestivum L.) using the AGLO strain containing the binary vector pBi-OAT with the target ornithine-δ-aminotransferase (oat) and selective neomycinphosphotransferase II (nptII), transgenic plants-regenerators have been obtained. Conclusions. As a result of the genetic transformation of Zimoyarka variety, 12 wheat regenerants were obtained in the genome which revealed a complete integration of the genetic construct containing the oat and nptII transgenes. Keywords: Triticum aestivum L., Agrobacterium-mediated transformation, ornithine‑δ‑aminotransferase gene, PCR-analysis.

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Some aspects of in vitro regeneration of bread wheat

Agronomie ◽

10.1051/agro:19950729 ◽

1995 ◽

Vol 15 (7-8) ◽

pp. 499-500

Author(s):

S. Bernard

Keyword(s):

Bread Wheat ◽

In Vitro Regeneration

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Effect of exposure time and incubation period of various sterilants and antioxidants on the in vitro morphogenesis of guava explants

IOSR Journal of Agriculture and Veterinary Science ◽

10.9790/2380-07548186 ◽

2014 ◽

Vol 7 (5) ◽

pp. 81-86

Author(s):

Roshan Zamir ◽

◽

Abdur- Rab

Keyword(s):

Incubation Period ◽

Exposure Time ◽

In Vitro Morphogenesis

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Establishment and characterization of conditionally immortalized cells from the mouse urogenital ridge

Journal of Cell Science ◽

10.1242/jcs.109.5.899 ◽

1996 ◽

Vol 109 (5) ◽

pp. 899-909 ◽

Cited By ~ 2

Author(s):

B. Capel ◽

J.R. Hawkins ◽

E. Hirst ◽

D. Kioussis ◽

R. Lovell-Badge

Keyword(s):

Primordial Germ Cells ◽

Cell Types ◽

T Antigen ◽

Temperature Sensitive ◽

Sv40 Large T Antigen ◽

In Vitro Morphogenesis ◽

Immortalized Cells ◽

Clonal Lines

Cell cultures from the urogenital ridge have been established to facilitate the study of the regulation and downstream interactions of Sry in mammalian sex determination. Cells have been explanted from transgenic mice carrying a temperature sensitive SV40 large T-antigen, and established in ongoing cultures. Analysis of the cells in these cultures at the electron microscope level reveals multiple cell types that compare to the cell types found in vivo during this period of development. Primordial germ cells, that are simultaneously explanted in the course of these experiments, also survive in culture. The explants undergo a morphogenetic organization into branching cord-like structures when cells are trypsinized and plated in extracellular matrix (Matrigel). We analyzed the expression of a number of molecular markers of the fetal gonad during monolayer culture, during in vitro morphogenesis in Matrigel, and in clonal lines derived from the complex explants. This analysis included Sry which is found to be expressed in some cultures from XY urogenital ridges that have been maintained for as long as 8 months.

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