scholarly journals The role of carbon and nitrogen sources in the production of bioactive compounds in Monascus fermentation products: a mini review

2021 ◽  
Vol 924 (1) ◽  
pp. 012083
Author(s):  
V T Widayanti ◽  
T Estiasih ◽  
E Zubaidah ◽  
M Taher
Keyword(s):  
Bioactive Compounds ◽  
Nitrogen Sources ◽  
Nutritional Composition ◽  
Carbon And Nitrogen Sources ◽  
Fermentation Products ◽  
Carbon And Nitrogen ◽  
Fermented Product ◽  
Antihyperlipidemic Activity ◽  
The Right

Abstract Angkak is one of the Monascus fermented products with rice as a substrate. In addition, Chinese yam, potatoes, soybean, ginseng and onions can be used as a substrate for fermentation products by Monascus sp. It was known that the fermented product by Monascus sp. produces several bioactive compounds that have antihyperlipidemic activity. These bioactive compounds are monacolin-K and pigment compounds (monascin and ankaflavin). Each of these compounds has different cholesterol inhibitory activity. The production of these bioactive compounds is strongly influenced by the nutritional composition of the fermentation media. The addition of the right carbon and nitrogen sources can accelerate the production of bioactive compounds by Monascus sp.

scholarly journals Regulation and Physiological Role of theDAS1 Gene, Encoding Dihydroxyacetone Synthase, in the Methylotrophic Yeast Candida boidinii

1998 ◽  
Vol 180 (22) ◽  
pp. 5885-5890 ◽  
Author(s):  
Yasuyoshi Sakai ◽  
Tomoyuki Nakagawa ◽  
Masayuki Shimase ◽  
Nobuo Kato
Keyword(s):  
Formate Dehydrogenase ◽  
Physiological Role ◽  
Nitrogen Sources ◽  
Host Genome ◽  
Candida Boidinii ◽  
Carbon And Nitrogen Sources ◽  
Gene Encoding ◽  
Carbon And Nitrogen ◽  
Dihydroxyacetone Synthase

ABSTRACT The physiological role of dihydroxyacetone synthase (DHAS) inCandida boidinii was evaluated at the molecular level. TheDAS1 gene, encoding DHAS, was cloned from the host genome, and regulation of its expression by various carbon and nitrogen sources was analyzed. Western and Northern analyses revealed thatDAS1 expression was regulated mainly at the mRNA level. The regulatory pattern of DHAS was similar to that of alcohol oxidase but distinct from that of two other enzymes in the formaldehyde dissimilation pathway, glutathione-dependent formaldehyde dehydrogenase and formate dehydrogenase. The DAS1 gene was disrupted in one step in the host genome (das1Δ strain), and the growth of the das1Δ strain in various carbon and nitrogen sources was compared with that of the wild-type strain. Thedas1Δ strain had completely lost the ability to grow on methanol, while the strain with a disruption of the formate dehydrogenase gene could survive (Y. Sakai et al., J. Bacteriol. 179:4480–4485, 1997). These and other experiments (e.g., those to determine the expression of the gene and the growth ability of thedas1Δ strain on media containing methylamine or choline as a nitrogen source) suggested that DAS1 is involved in assimilation rather than dissimilation or detoxification of formaldehyde in the cells.

scholarly journals Role of Actinomycete sp. in Bio-extraction of Copper from Electronic Waste

2021 ◽  
Vol 12 (5) ◽  
pp. 6723-6740
Keyword(s):  
Printed Circuit Boards ◽  
Electronic Waste ◽  
Nitrogen Sources ◽  
Dissolution Mechanism ◽  
Circuit Boards ◽  
Carbon And Nitrogen Sources ◽  
Nitrate Medium ◽  
Carbon And Nitrogen ◽  
Printed Circuit

Copper is bioleached from printed circuit boards (PCBs) using Actinomycete sp. It was identified by 16S rRNA, called Streptomyces graminofaciens. The tolerance of S. graminofaciens towards copper showed that it couldn’t live in the presence of copper sulfate. The process was carried out by culturing 3.09×103 CFU of S. graminofaciens in 50 ml of modified starch nitrate medium for three days at 200 rpm and 30C and then 0.5% e-waste was added for other 5 days. Glucose (0.01 g/ml) and ammonium sulfate (0.002g/ml) were the best carbon and nitrogen sources. At pH 5, the leached copper was 88.1%. The bio-dissolution mechanism was investigated via the production of enzymes of S. graminofaciens metabolites, which showed that the higher dissolution has occurred in an un-denatured sample (non-heating) than the denatured one (heating). FTIR spectra confirmed the action of S. graminofaciens through the disappearance and appearance of some peaks. SEM showed that the e-waste gained more pores as a result of bio-treatment, which refers to the liberation of metals in solution.

scholarly journals High level production of laccases and peroxidases from the newly isolated white-rot basidiomycete Marasmiellus palmivorus VE111 in a stirred-tank bioreactor in response to different carbon and nitrogen sources

2018 ◽  
Vol 69 ◽  
pp. 1-11 ◽  
Author(s):  
Willian Daniel Hahn Schneider ◽  
Roselei Claudete Fontana ◽  
Simone Mendonça ◽  
Félix Gonçalves de Siqueira ◽  
Aldo José Pinheiro Dillon ◽  
...  
Keyword(s):  
Nitrogen Sources ◽  
White Rot ◽  
Stirred Tank ◽  
Carbon And Nitrogen Sources ◽  
Stirred Tank Bioreactor ◽  
Carbon And Nitrogen ◽  
High Level ◽  
Level Production
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