Gradient porous hydroxyapatite ceramics fabricated by freeze casting method

In this study, hydroxyapatite nanoparticles containing 10% doxycycline, a structural isomer of tetracycline, was prepared by the co-precipitation method. It was added to collagen solution for the preparation of the scaffold with freeze-casting method in order to develop a composite scaffold with both antibacterial and osteoinductive properties for repairing bone defects. The scaffolds were evaluated regarding their morphology, porosity, degradation and cellular response. The scaffolds for further investigation were added in a rat calvaria defect model. The study showed that after eight weeks, the bone formation was relatively higher in the collagen/nano-hydroxyapatite/doxycycline group with completely filled defect when compared with other groups. Histopathological evaluation showed that the defect in the collagen/nano-hydroxyapatite/doxycycline group was fully replaced by the new bone and connective tissue. Our results provide evidence supporting the possible applicability of doxycycline-containing scaffolds for successful bone regeneration.

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Synthesis, characterization and biocompatibility evaluation of sol–gel derived bioactive glass scaffolds prepared by freeze casting method

Ceramics International ◽

10.1016/j.ceramint.2013.10.115 ◽

2014 ◽

Vol 40 (4) ◽

pp. 5349-5355 ◽

Cited By ~ 24

Author(s):

M. Mozafari ◽

F. Moztarzadeh

Keyword(s):

Bioactive Glass ◽

Sol Gel ◽

Freeze Casting ◽

Casting Method

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Preparation of a Hydroxyapatite Ceramic with Comblike Tubules Structure and its Permeablity

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.768.135 ◽

2018 ◽

Vol 768 ◽

pp. 135-139 ◽

Cited By ~ 1

Author(s):

Xin Yi Zhao ◽

Xiao Peng Li ◽

Kang Zhao ◽

Yu Fei Tang

Keyword(s):

Cross Section ◽

Longitudinal Section ◽

Hydraulic Conductance ◽

Hydroxyapatite Ceramic ◽

Porous Hydroxyapatite ◽

Dentin Tubule ◽

Hydroxyapatite Powder ◽

Hydroxyapatite Ceramics ◽

Porous Hydroxyapatite Ceramic ◽

C 30

The objectives of this study are to prepare a porous hydroxyapatite ceramic which has dentin tubule-like structure and determine its permeability. Slurry of hydroxyapatite powder, deionized water and a binder was poured into a ring which was placed on a freezing plate.The hydroxyapatite slurry was freezed in a certain rate (by controlling the temperature of the freeze plate at −15°C, −30°C and −45°C) for a certain period of time, then the freezed sample was freezing dried to remove the frozen vehicle, followed by being sintered at 1250 °C for 2 h. After that，the morphology of the cross section and longitudinal section of the sintered porous hydroxyapatite ceramic was observed by SEM and the hydraulic conductance of cross section discs of the sintered porous hydroxyapatite were determined using a self-made micro-flowing permeability tester. Results showed that the prepared hydroxyapatite ceramics having bottom-up unidirectional comblike tubule structure and the tubule diameters associated with the temperature of freezing plate.The ceramic discs prepared on the freezing plate of −45°C exhibited similarity to nature dentin tubule, with a diameter of 9.72±3.41mm and a hydraulic conductance of 0.16±0.09 ml×min-1×cm-2×cm×H2O-1.

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