Stimulation of Reparative Dentin Formation by Ex Vivo Gene Therapy Using Dental Pulp Stem Cells Electrotransfected with Growth/differentiation factor 11 (Gdf11)

2004 ◽  
Vol 15 (11) ◽  
pp. 1045-1053 ◽  
Author(s):  
Misako Nakashima ◽  
Koichiro Iohara ◽  
Masaki Ishikawa ◽  
Masataka Ito ◽  
Atsushi Tomokiyo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Therapy ◽  
Dental Pulp ◽  
Ex Vivo ◽  
Dental Pulp Stem Cells ◽  
Reparative Dentin ◽  
Dentin Formation ◽  
I Gene ◽  
Reparative Dentin Formation ◽  
Stimulation Of

scholarly journals Evaluation of Chitosan Hydrogel for Sustained Delivery of VEGF for Odontogenic Differentiation of Dental Pulp Stem Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
Si Wu ◽  
Yachuan Zhou ◽  
Yi Yu ◽  
Xin Zhou ◽  
Wei Du ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Sol Gel ◽  
Dental Pulp Stem Cells ◽  
Bioactive Molecules ◽  
Pulp Capping ◽  
Odontogenic Differentiation ◽  
Reparative Dentin ◽  
Porous Microstructure ◽  
Dentin Formation

The pulpotomy with pulp capping is aimed at retaining vital pulp with reparative dentin formation. Vascular endothelial growth factor (VEGF) plays a crucial role in dentin regeneration; however, its constant administrations in the human body is still problematic. Chitosan was widely studied as an effective carrier to deliver bioactive molecules in regenerative medicine. In this study, we conducted a chitosan/β-glycerophosphate (CS/β-GP) hydrogel as a VEGF-sustained release system and explored its effects on dental pulp stem cells (DPSCs). CS/β-GP hydrogel was manufactured using a sol-gel method. SEM assay showed the spongy and porous microstructure of the lyophilized hydrogels. DPSCs cultured in the CS/β-GP hydrogel kept adhesion and vitality. CCK-8 assay tested the promoted proliferation activity of DPSCs on the hydrogel. Besides, the added VEGF protein could continually release from VEGF/CS/β-GP hydrogel. The VEGF/CS/β-GP hydrogel could promote the odontogenic differentiation of DPSCs better than VEGF treatment without hydrogel. Our results suggested that CS/β-GP hydrogel could continually release VEGF and contribute to odontogenic differentiation of DPSCs, thus may become a potential carrier of bioactive molecules in pulp capping therapy.

scholarly journals Lithium Chloride Exerts Differential Effects on Dentinogenesis and Osteogenesis in Primary Pulp Cultures

2021 ◽  
Vol 2 ◽  
Author(s):  
Anushree Vijaykumar ◽  
Mina Mina
Keyword(s):  
Dental Pulp ◽  
Osteoblast Differentiation ◽  
Prolonged Treatment ◽  
Odontoblast Differentiation ◽  
Positive Effects ◽  
Reparative Dentin ◽  
Dentin Formation ◽  
Reparative Dentin Formation

Wnt/β-catenin signaling is known to play essential roles in odontoblast differentiation and reparative dentin formation. Various Wnt activators including LiCl have been increasingly studied for their effectiveness to induce repair of the dentin-pulp complex. LiCl is a simple salt thought to activate Wnt/β-catenin signaling by inhibiting GSK3β. Previous in vitro and in vivo studies showed that LiCl increased odontoblast differentiation and enhanced reparative dentin formation. However, the underlying molecular and cellular mechanisms by which LiCl regulates odontoblast and osteoblast differentiation during reparative dentinogenesis are not well-understood. Our in vitro studies show that exposure of early dental pulp progenitors to LiCl increased the survival and the pool of αSMA+ progenitors, leading to enhanced odontoblast and osteoblast differentiation. The positive effects of LiCl in the differentiation of osteoblasts and odontoblasts from αSMA+ progenitors are mediated by Wnt/β-catenin signaling. Our results also showed that continuous and late exposure of dental pulp cells to LiCl increased the expression of odontoblast markers through Wnt/β-catenin signaling, and the number of odontoblasts expressing DMP1-Cherry and DSPP-Cerulean transgenes. However, unlike the early treatment, both continuous and late treatments decreased the expression of Bsp and the expression of BSP-GFPtpz transgene. These observations suggest that prolonged treatment with LiCl in more mature cells of the dental pulp has an inhibitory effect on osteoblast differentiation. The inhibitory effects of LiCl on osteogenesis and Bsp were not mediated through Wnt/β-catenin signaling. These observations suggest that the effects of LiCl, and GSK3β antagonists on reparative dentinogenesis involve multiple pathways and are not specific to Wnt/β-catenin signaling.

Templated dentin formation by dental pulp stem cells on banded collagen bundles nucleated on electrospun poly (4-vinyl pyridine) fibers in vitro

2018 ◽  
Vol 76 ◽  
pp. 80-88 ◽  
Author(s):  
Linxi Zhang ◽  
Yingjie Yu ◽  
Kuan-che Feng ◽  
Ya-chen Chuang ◽  
Xianghao Zuo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Vinyl Pyridine ◽  
Dentin Formation

scholarly journals Secreted Frizzled-Related Protein 1 Promotes Odontoblastic Differentiation and Reparative Dentin Formation in Dental Pulp Cells

Cells ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2491
Author(s):  
Keita Ipposhi ◽  
Atsushi Tomokiyo ◽  
Taiga Ono ◽  
Kozue Yamashita ◽  
Muhammad Anas Alhasan ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Nodule Formation ◽  
Dental Pulp Cells ◽  
Related Protein ◽  
Direct Pulp Capping ◽  
Odontoblastic Differentiation ◽  
Reparative Dentin ◽  
Pulp Cells ◽  
Dentin Formation ◽  
Reparative Dentin Formation

Direct pulp capping is an effective treatment for preserving dental pulp against carious or traumatic pulp exposure via the formation of protective reparative dentin by odontoblast-like cells. Reparative dentin formation can be stimulated by several signaling molecules; therefore, we investigated the effects of secreted frizzled-related protein (SFRP) 1 that was reported to be strongly expressed in odontoblasts of newborn molar tooth germs on odontoblastic differentiation and reparative dentin formation. In developing rat incisors, cells in the dental pulp, cervical loop, and inner enamel epithelium, as well as ameloblasts and preodontoblasts, weakly expressed Sfrp1; however, Sfrp1 was strongly expressed in mature odontoblasts. Human dental pulp cells (hDPCs) showed stronger expression of SFRP1 compared with periodontal ligament cells and gingival cells. SFRP1 knockdown in hDPCs abolished calcium chloride-induced mineralized nodule formation and odontoblast-related gene expression and decreased BMP-2 gene expression. Conversely, SFRP1 stimulation enhanced nodule formation and expression of BMP-2. Direct pulp capping treatment with SFRP1 induced the formation of a considerable amount of reparative dentin that has a structure similar to primary dentin. Our results indicate that SFRP1 is crucial for dentinogenesis and is important in promoting reparative dentin formation in response to injury.

Pulp Stem Cells: Implication in Reparative Dentin Formation

2014 ◽  
Vol 40 (4) ◽  
pp. S13-S18 ◽  
Author(s):  
Sasha Dimitrova-Nakov ◽  
Anne Baudry ◽  
Yassine Harichane ◽  
Odile Kellermann ◽  
Michel Goldberg
Keyword(s):  
Stem Cells ◽  
Reparative Dentin ◽  
Dentin Formation ◽  
Reparative Dentin Formation

scholarly journals Telomere Attrition Occurs during Ex Vivo Expansion of Human Dental Pulp Stem Cells

2010 ◽  
Vol 2010 ◽  
pp. 1-11 ◽  
Author(s):  
Jaroslav Mokry ◽  
Tomas Soukup ◽  
Stanislav Micuda ◽  
Jana Karbanova ◽  
Benjamin Visek ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Telomere Length ◽  
Dental Pulp ◽  
Ex Vivo ◽  
Ex Vivo Expansion ◽  
Dental Pulp Stem Cells ◽  
Cell Markers ◽  
Relative Telomere Length ◽  
Human Dental Pulp

We provide a detailed characteristic of stem cells isolated and expanded from the human dental pulp. Dental pulp stem cells express mesenchymal cell markers STRO-1, vimentin, CD29, CD44, CD73, CD90, CD166, and stem cell markers Sox2, nestin, and nucleostemin. They are multipotent as shown by their osteogenic and chondrogenic potential. We measured relative telomere length in 11 dental pulp stem cell lines at different passages by quantitative real-time PCR. Despite their large proliferative capacity, stable viability, phenotype, and genotype over prolonged cultivation, human dental pulp stem cells suffer from progressive telomere shortening over time they replicate in vitro. Relative telomere length (T/S) was inversely correlated with cumulative doubling time. Our findings indicate that excessive ex vivo expansion of adult stem cells should be reduced at minimum to avoid detrimental effects on telomere maintenance and measurement of telomere length should become a standard when certificating the status and replicative age of stem cells prior therapeutic applications.

Differentiation of Human Dental Pulp Stem Cells into Functional Motor Neuron; In vitro and Ex vivo Study

2021 ◽  
pp. 101542
Author(s):  
Marzieh Darvishi ◽  
Hatef Ghasemi Hamidabadi ◽  
Maryam Nazm Bojnordi ◽  
Sara saeednia ◽  
Maria Zahiri ◽  
...  
Keyword(s):  
Stem Cells ◽  
Motor Neuron ◽  
Dental Pulp ◽  
Ex Vivo ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp ◽  
Ex Vivo Study
Sign in / Sign up

Export Citation Format

Share Document