Expression and Immunologic Characterization of Recombinant Heat Shock Protein 58 of Leptospira Species: A Major Target Antigen of the Humoral Immune Response

1999 ◽  
Vol 18 (12) ◽  
pp. 903-910 ◽  
Author(s):  
Se-Hoon Park ◽  
Byung-Yoon Ahn ◽  
Min-Ja Kim
Keyword(s):  
Immune Response ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Humoral Immune Response ◽  
Target Antigen ◽  
Humoral Immune ◽  
Leptospira Species ◽  
Major Target

Humoral immune response to the 72 kDa heat shock protein from Plasmodium falciparum in populations at hypoendemic areas of malaria in western Brazilian Amazon

Acta Tropica ◽  
1997 ◽  
Vol 64 (3-4) ◽  
pp. 155-166 ◽  
Author(s):  
Cláudio O.P Alexandre ◽  
Luis Marcelo Aranha Camargo ◽  
Denise Mattei ◽  
Marcelo U Ferreira ◽  
Alejandro M Katzin ◽  
...  
Keyword(s):  
Immune Response ◽  
Plasmodium Falciparum ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Humoral Immune Response ◽  
Brazilian Amazon ◽  
Humoral Immune

scholarly journals Humoral immune response to mycobacterial heat shock protein (hsp)65 in the cerebrospinal fluid of neuro-Behçet patients

1998 ◽  
Vol 113 (1) ◽  
pp. 100-104 ◽  
Author(s):  
B. TaŞÇi ◽  
H. Direskeneli ◽  
P. Serdaroğlu ◽  
G. Akman-Demir ◽  
M. Eraksoy ◽  
...  
Keyword(s):  
Immune Response ◽  
Cerebrospinal Fluid ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Humoral Immune Response ◽  
Humoral Immune

scholarly journals Characterization of Lassa Virus Cell Entry and Neutralization with Lassa Virus Pseudoparticles

2009 ◽  
Vol 83 (7) ◽  
pp. 3228-3237 ◽  
Author(s):  
François-Loic Cosset ◽  
Philippe Marianneau ◽  
Geraldine Verney ◽  
Fabrice Gallais ◽  
Noel Tordo ◽  
...  
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Neutralizing Antibodies ◽  
Cell Attachment ◽  
Low Ph ◽  
Cell Entry ◽  
Lassa Virus ◽  
Ph Optimum ◽  
Humoral Immune

ABSTRACT The cell entry and humoral immune response of the human pathogen Lassa virus (LV), a biosafety level 4 (BSL4) Old World arenavirus, are not well characterized. LV pseudoparticles (LVpp) are a surrogate model system that has been used to decipher factors and routes involved in LV cell entry under BSL2 conditions. Here, we describe LVpp, which are highly infectious, with titers approaching those obtained with pseudoparticles displaying G protein of vesicular stomatitis virus and their the use for the characterization of LV cell entry and neutralization. Upon cell attachment, LVpp utilize endocytic vesicles for cell entry as described for many pH-dependent viruses. However, the fusion of the LV glycoproteins is activated at unusually low pH values, with optimal fusion occurring between pH 4.5 and 3, a pH range at which fusion characteristics of viral glycoproteins have so far remained largely unexplored. Consistent with a shifted pH optimum for fusion activation, we found wild-type LV and LVpp to display a remarkable resistance to exposure to low pH. Finally, LVpp allow the fast and quantifiable detection of neutralizing antibodies in human and animal sera and will thus facilitate the study of the humoral immune response in LV infections.

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