Cloning and Characterization of the Bovine Gene for Steroid 21-Hydroxylase (P-450c21)

DNA ◽  
1985 ◽  
Vol 4 (3) ◽  
pp. 211-219 ◽  
Author(s):  
BON-CHU CHUNG ◽  
KARLA J. MATTESON ◽  
WALTER L. MILLER
Keyword(s):  
Bovine Gene

Isolation and characterization of a bovine gene encoding phenol sulfotransferase

Gene ◽  
1996 ◽  
Vol 174 (2) ◽  
pp. 221-224 ◽  
Author(s):  
Travis Henry ◽  
Brian Kliewer ◽  
Robert Palmatier ◽  
Joseph S. Ulphani ◽  
Joe D. Beckmann
Keyword(s):  
Gene Encoding ◽  
Bovine Gene ◽  
Isolation And Characterization ◽  
Phenol Sulfotransferase

scholarly journals Characterization of the cDNA and genomic sequence of a G protein gamma subunit (gamma 5).

1992 ◽  
Vol 12 (4) ◽  
pp. 1585-1591 ◽  
Author(s):  
K J Fisher ◽  
N N Aronson
Keyword(s):  
G Protein ◽  
Genomic Sequence ◽  
Cdna Clones ◽  
Gamma Subunit ◽  
Bovine Gene ◽  
Gamma Subunits ◽  
Sequence Encoding ◽  
Rat Livers ◽  
Exon Structure

A cDNA from human placenta and liver tissues that contained both sequence for the lysosomal glycosidase di-N-acetylchitobiase and sequence homologous to the gamma subunit of GTP-binding proteins was previously isolated. Here we have shown that the gamma-subunit-homologous portion of this unusual cDNA is derived from a member of the gamma-subunit multigene family. The partial human gamma-subunit sequence was used to isolate the corresponding full-length cDNA clones from bovine and rat livers. The two cDNAs encode identical 68-amino-acid proteins (7.3 kDa) homologous to previously cloned G protein gamma subunits. The bovine gene sequence encoding this new gamma-subunit isoform (gamma 5) was determined and found to have an intron-exon structure consistent with the original human chitobiase-gamma 5-subunit hybrid mRNA being a product of alternative splicing. Genomic cloning also resulted in the isolation of a human gamma 5 pseudogene.

Characterization of the cDNA and genomic sequence of a G protein gamma subunit (gamma 5)

1992 ◽  
Vol 12 (4) ◽  
pp. 1585-1591
Author(s):  
K J Fisher ◽  
N N Aronson
Keyword(s):  
G Protein ◽  
Genomic Sequence ◽  
Cdna Clones ◽  
Gamma Subunit ◽  
Bovine Gene ◽  
Gamma Subunits ◽  
Sequence Encoding ◽  
Rat Livers ◽  
Exon Structure

A cDNA from human placenta and liver tissues that contained both sequence for the lysosomal glycosidase di-N-acetylchitobiase and sequence homologous to the gamma subunit of GTP-binding proteins was previously isolated. Here we have shown that the gamma-subunit-homologous portion of this unusual cDNA is derived from a member of the gamma-subunit multigene family. The partial human gamma-subunit sequence was used to isolate the corresponding full-length cDNA clones from bovine and rat livers. The two cDNAs encode identical 68-amino-acid proteins (7.3 kDa) homologous to previously cloned G protein gamma subunits. The bovine gene sequence encoding this new gamma-subunit isoform (gamma 5) was determined and found to have an intron-exon structure consistent with the original human chitobiase-gamma 5-subunit hybrid mRNA being a product of alternative splicing. Genomic cloning also resulted in the isolation of a human gamma 5 pseudogene.

scholarly journals Characterization of the bovine gene LIPE and possible influence on fatty acid composition of meat

Meta Gene ◽  
2014 ◽  
Vol 2 ◽  
pp. 746-760 ◽  
Author(s):  
Daniel Estanislao Goszczynski ◽  
Juliana Papaleo Mazzucco ◽  
María Verónica Ripoli ◽  
Edgardo Leopoldo Villarreal ◽  
Andrés Rogberg-Muñoz ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Bovine Gene

Microfibril-associated glycoprotein: Characterization of the bovine gene and of the recombinantly expressed protein

Biochemistry ◽  
1994 ◽  
Vol 33 (2) ◽  
pp. 593-600 ◽  
Author(s):  
Muhammad M. Bashir ◽  
William R. Abrams ◽  
Joan Rosenbloom ◽  
Umberto Kucich ◽  
Michelle Bacarra ◽  
...  
Keyword(s):  
Bovine Gene

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

AEM analysis of crystallization in Ti-based amorphous alloys

1983 ◽  
Vol 41 ◽  
pp. 270-271
Author(s):  
A.R. Pelton ◽  
A.F. Marshall ◽  
Y.S. Lee
Keyword(s):  
Magnetic Properties ◽  
Amorphous Alloys ◽  
Amorphous Materials ◽  
Isothermal Annealing ◽  
Amorphous Matrix ◽  
Nucleation And Growth ◽  
Current Interest ◽  
Amorphous Films ◽  
Electrical And Magnetic Properties

Amorphous materials are of current interest due to their desirable mechanical, electrical and magnetic properties. Furthermore, crystallizing amorphous alloys provides an avenue for discerning sequential and competitive phases thus allowing access to otherwise inaccessible crystalline structures. Previous studies have shown the benefits of using AEM to determine crystal structures and compositions of partially crystallized alloys. The present paper will discuss the AEM characterization of crystallized Cu-Ti and Ni-Ti amorphous films.Cu60Ti40: The amorphous alloy Cu60Ti40, when continuously heated, forms a simple intermediate, macrocrystalline phase which then transforms to the ordered, equilibrium Cu3Ti2 phase. However, contrary to what one would expect from kinetic considerations, isothermal annealing below the isochronal crystallization temperature results in direct nucleation and growth of Cu3Ti2 from the amorphous matrix.

Characterization of Coherent, Ordered Precipitates in Nickel-Base Alloys

1973 ◽  
Vol 31 ◽  
pp. 144-145
Author(s):  
B. H. Kear ◽  
J. M. Oblak
Keyword(s):  
Stable Phase ◽  
Nickel Base Superalloy ◽  
Alloy 718 ◽  
Commercial Alloy ◽  
Precipitate Morphology ◽  
Continuous Precipitation ◽  
Nickel Base ◽  
Base Superalloy ◽  
Strengthening Precipitate

A nickel-base superalloy is essentially a Ni/Cr solid solution hardened by additions of Al (Ti, Nb, etc.) to precipitate a coherent, ordered phase. In most commercial alloy systems, e.g. B-1900, IN-100 and Mar-M200, the stable precipitate is Ni3 (Al,Ti) γ′, with an LI2structure. In A lloy 901 the normal precipitate is metastable Nis Ti3 γ′ ; the stable phase is a hexagonal Do2 4 structure. In Alloy 718 the strengthening precipitate is metastable γ″, which has a body-centered tetragonal D022 structure.Precipitate MorphologyIn most systems the ordered γ′ phase forms by a continuous precipitation re-action, which gives rise to a uniform intragranular dispersion of precipitate particles. For zero γ/γ′ misfit, the γ′ precipitates assume a spheroidal.

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