Feijoa sellowiana Berg Fruit Juice: Anti-Inflammatory Effect and Activity on Superoxide Anion Generation

Chemical investigation of a Red Sea Spongia sp. led to the isolation of four new compounds, i.e., 17-dehydroxysponalactone (1), a carboxylic acid, spongiafuranic acid A (2), one hydroxamic acid, spongiafuranohydroxamic acid A (3), and a furanyl trinorsesterpenoid 16-epi-irciformonin G (4), along with three known metabolites (−)-sponalisolide B (5), 18-nor- 3,17-dihydroxy-spongia-3,13(16),14-trien-2-one (6), and cholesta-7-ene-3β,5α-diol-6-one (7). The biosynthetic pathway for the molecular skeleton of 1 and related compounds was postulated for the first time. Anti-inflammatory activity of these metabolites to inhibit superoxide anion generation and elastase release in N-formyl-methionyl-leucyl phenylalanine/cytochalasin B (fMLF/CB)-induced human neutrophil cells and cytotoxicity of these compounds toward three cancer cell lines and one human dermal fibroblast cell line were assayed. Compound 1 was found to significantly reduce the superoxide anion generation and elastase release at a concentration of 10 μM, and compound 5 was also found to display strong inhibitory activity against superoxide anion generation at the same concentration. Due to the noncytotoxic activity and the potent inhibitory effect toward the superoxide anion generation and elastase release, 1 and 5 can be considered to be promising anti-inflammatory agents.

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Capping of silver nanoparticles by anti-inflammatory ligands: Antibacterial activity and superoxide anion generation

Journal of Photochemistry and Photobiology B Biology ◽

10.1016/j.jphotobiol.2019.02.005 ◽

2019 ◽

Vol 193 ◽

pp. 100-108 ◽

Cited By ~ 6

Author(s):

Manuel I. Azócar ◽

Romina Alarcón ◽

Antonio Castillo ◽

Jenny M. Blamey ◽

Mariana Walter ◽

...

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Superoxide Anion ◽

Superoxide Anion Generation ◽

Anti Inflammatory

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Phytochemical Investigation and Anti-Inflammatory Activity of the Leaves of Machilus japonica var. kusanoi

Molecules ◽

10.3390/molecules25184149 ◽

2020 ◽

Vol 25 (18) ◽

pp. 4149

Author(s):

Shiou-Ling Li ◽

Ho-Cheng Wu ◽

Tsong-Long Hwang ◽

Chu-Hung Lin ◽

Shuen-Shin Yang ◽

...

Keyword(s):

Superoxide Anion ◽

2D Nmr ◽

Human Neutrophils ◽

Superoxide Anion Generation ◽

Phytochemical Investigation ◽

Potent Inhibition ◽

Bioassay Guided Fractionation ◽

Ic50 Values ◽

Anti Inflammatory ◽

New Compounds

In a series of anti-inflammatory screenings of lauraceous plants, the methanolic extract of the leaves of Machilus japonica var. kusanoi (Hayata) J.C. Liao showed potent inhibition on both superoxide anion generation and elastase release in human neutrophils. Bioassay-guided fractionation of the leaves of M. japonica var. kusanoi led to the isolation of twenty compounds, including six new butanolides, machinolides A–F (1–6), and fourteen known compounds (7–20). Their structures were characterized by 1D and 2D NMR, UV, IR, CD, and MS data. The absolute configuration of the new compounds were unambiguously confirmed by single-crystal X-ray diffraction analyses (1, 2, and 3) and Mosher’s method (4, 5, and 6). In addition, lignans, (+)-eudesmin (11), (+)-methylpiperitol (12), (+)-pinoresinol (13), and (+)-galbelgin (16) exhibited inhibitory effects on N-formyl-methionyl-leucyl-phenylalanine/cytochalasin B (fMLP/CB)-induced superoxide anion generation in human neutrophils with IC50 values of 8.71 ± 0.74 μM, 2.23 ± 0.92 μM, 6.81 ± 1.07 μM, and 7.15 ± 2.26 μM, respectively. The results revealed the anti-inflammatory potentials of Formosan Machilus japonica var. kusanoi.

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Constituents from the Fruiting Bodies of Trametes cubensis and Trametes suaveolens in Vietnam and Their Anti-Inflammatory Bioactivity

Molecules ◽

10.3390/molecules26237311 ◽

2021 ◽

Vol 26 (23) ◽

pp. 7311

Author(s):

Yue-Chiun Li ◽

Nguyen Thi Ngan ◽

Kun-Ching Cheng ◽

Tsong-Long Hwang ◽

Tran Dinh Thang ◽

...

Keyword(s):

Superoxide Anion ◽

Analytical Data ◽

Significant Inhibition ◽

Human Neutrophils ◽

Spectrometric Analysis ◽

Fruiting Bodies ◽

Edible Fungi ◽

Superoxide Anion Generation ◽

Isolation And Characterization ◽

Anti Inflammatory

It is reported that various fungi have been used for medicine and edible foods. The tropical Trametes genus is popular and well-known in Vietnam for its health effects and bioactivities. In this study, the fruiting bodies of the edible fungi T. cubensis and T. suaveolens were collected in Vietnam. The preliminary bioactivity screening data indicated that the methanol extracts of the fruiting bodies of T. cubensis and T. suaveolens displayed significant inhibition of superoxide anion generation and elastase release in human neutrophils. Therefore, the isolation and characterization were performed on these two species by a combination of chromatographic methods and spectrometric analysis. In total, twenty-four compounds were identified, and among these (1–3) were characterized by 1D-, 2D-NMR, and HRMS analytical data. In addition, the anti-inflammatory potentials of some purified compounds were examined by the cellular model for the inhibition of superoxide anion generation and elastase release in human neutrophils. Among the isolated compounds, (5,14), and (19) displayed significant anti-inflammatory potential. As the results suggest, the extracts and isolated compounds from T. cubensis and T. suaveolens are potential candidates for the further development of new anti-inflammatory lead drugs or natural healthy foods.

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Anti-Inflammatory Cembranoids from a Formosa Soft Coral Sarcophyton cherbonnieri

Marine Drugs ◽

10.3390/md18110573 ◽

2020 ◽

Vol 18 (11) ◽

pp. 573

Author(s):

Chia-Chi Peng ◽

Chiung-Yao Huang ◽

Atallah F. Ahmed ◽

Tsong-Long Hwang ◽

Jyh-Horng Sheu

Keyword(s):

Superoxide Anion ◽

Soft Coral ◽

Chemical Reduction ◽

Chemical Constituents ◽

2D Nmr ◽

Human Neutrophils ◽

Superoxide Anion Generation ◽

Chemical Structures ◽

Proinflammatory Responses ◽

Anti Inflammatory

The present investigation on chemical constituents of the soft coral Sarcophyton cherbonnieri resulted in the isolation of seven new cembranoids, cherbonolides F–L (1–7). The chemical structures of 1–7 were determined by spectroscopic methods, including infrared, one- and two-dimensional (1D and 2D) NMR (COSY, HSQC, HMBC, and NOESY), MS experiments, and a chemical reduction of hydroperoxide by triphenylphosphine. The anti-inflammatory activities of 1–7 against neutrophil proinflammatory responses were evaluated by measuring their inhibitory ability toward N-formyl-methionyl-leucyl-phenylalanine/cytochalasin B (fMLF/CB)-induced superoxide anion generation and elastase release in primary human neutrophils. The results showed that all isolates exhibited moderate activities, while cherbonolide G (2) and cherbonolide H (3) displayed a more active effect than others on the inhibition of elastase release (48.2% ± 6.2%) and superoxide anion generation (44.5% ± 4.6%) at 30 µM, respectively.

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Clerodane Diterpenoids from Callicarpa hypoleucophylla and Their Anti-Inflammatory Activity

Molecules ◽

10.3390/molecules25102288 ◽

2020 ◽

Vol 25 (10) ◽

pp. 2288 ◽

Cited By ~ 2

Author(s):

Yu-Chi Lin ◽

Jue-Jun Lin ◽

Shu-Rong Chen ◽

Tsong-Long Hwang ◽

Shu-Yen Fang ◽

...

Keyword(s):

Nuclear Magnetic Resonance ◽

Superoxide Anion ◽

Spectroscopic Data ◽

Structural Diversity ◽

Inflammatory Activity ◽

Endemic Plant ◽

Superoxide Anion Generation ◽

Phytochemical Investigation ◽

Clerodane Diterpenoids ◽

Anti Inflammatory

Plants of the genus Callicarpa are known to possess several medicinal effects. The constituents of the Taiwan endemic plant Callicarpa hypoleucophylla have never been studied. Therefore, C. hypoleucophylla was selected for our phytochemical investigation. Two new clerodane-type diterpenoids, named callihypolins A (1) and B (2), along with seven known compounds were isolated from the leaves and twigs of the Lamiaceae plant C. hypoleucophylla and then characterized. The structures of compounds 1 and 2 were elucidated by spectroscopic data analysis, specifically, two-dimension nuclear magnetic resonance (NMR). The anti-inflammatory activity of compounds 1–9 based on the suppression of superoxide anion generation and elastase release was evaluated. Among the isolates, compounds 2–4 showed anti-inflammatory activity (9.52−32.48% inhibition at the concentration 10 μm) by suppressing superoxide anion generation and elastase release. Our findings not only expand the description of the structural diversity of the compounds present in plants of the genus Callicarpa but also highlight the possibility of developing anti-inflammatory agents from Callicarpa endemic species.

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1H NMR-Based Isolation of Anti-Inflammatory 9,11-Secosteroids from the Octocoral Sinularia leptoclados

Marine Drugs ◽

10.3390/md18050271 ◽

2020 ◽

Vol 18 (5) ◽

pp. 271 ◽

Cited By ~ 1

Author(s):

Yu-Chia Chang ◽

Kuei-Hung Lai ◽

Sunil Kumar ◽

Po-Jen Chen ◽

Yi-Hsuan Wu ◽

...

Keyword(s):

1H Nmr ◽

Superoxide Anion ◽

Nmr Spectra ◽

Spectroscopic Analysis ◽

Pronounced Effect ◽

Neutrophilic Inflammation ◽

Superoxide Anion Generation ◽

Ic50 Values ◽

Anti Inflammatory

Octocoral Sinularia leptoclados has been identified as a source of bioactive 9,11-secosteroids. This study adopted a targeted isolation approach to the discovery and analysis of five 9,11-secosteroids, including two novel compounds named sinleptosterols A (1) and B (2) as well as five known analogues (8αH-3β,11-dihydroxy-24-methylene-9,11-secocholest-5-en-9-one (3), 8βH-3β,11-dihydroxy-24-methylene-9,11-secocholest-5-en-9-one (4), leptosterol A (5), (24S)-3β,11-dihydroxy-24-methyl-9,11-secocholest-5-en-9-one (6), and 3β,11-dihydroxy-9,11-secogorgost-5-en-9-one (7)) in terms of 1H-NMR patterns and potency against neutrophilic inflammation. The structure of secosteroids 1 and 2 was deduced from general spectroscopic analysis and an examination of NMR spectra. Among the above-mentioned isolates, compound 4 had the most pronounced effect in inhibiting elastase release and superoxide anion generation, with the IC50 values of 2.96 and 1.63 μM, respectively.

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Chemical Constituents from the Stems of Tinospora sinensis and Their Bioactivity

Molecules ◽

10.3390/molecules23102541 ◽

2018 ◽

Vol 23 (10) ◽

pp. 2541 ◽

Cited By ~ 8

Author(s):

Sio-Hong Lam ◽

Po-Hsun Chen ◽

Hsin-Yi Hung ◽

Tsong-Long Hwang ◽

Chih-Chao Chiang ◽

...

Keyword(s):

Analytical Methods ◽

Superoxide Anion ◽

Cytochalasin B ◽

Chemical Constituents ◽

Superoxide Anion Generation ◽

Anti Inflammatory ◽

New Compounds

Fifty-seven compounds were purified from the stems of Tinospora sinensis, including three new compounds characterized as a lignan (1), a pyrrole alkaloid (11), and a benzenoid (17), respectively. Their structures were elucidated and established by various spectroscopic and spectrometric analytical methods. Among the isolates, fifteen compounds were examined for their anti-inflammatory potential in vitro. The results showed that several compounds displayed moderate inhibition of N-formyl-methionyl-leucyl-phenylalanine/cytochalasin B (fMLP/CB)-induced superoxide anion generation and elastase release.

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Reduction of paw edema and liver oxidative stress in carrageenan-induced acute inflammation by Lobaria pulmonaria and Parmelia caperata, lichen species, in mice

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000620 ◽

2019 ◽

pp. 1-9

Author(s):

Samira Salem ◽

Essaid Leghouchi ◽

Rachid Soulimani ◽

Jaouad Bouayed

Keyword(s):

Time Course ◽

Acute Inflammation ◽

Lichen Species ◽

Paw Edema ◽

Sod Activity ◽

Edema Formation ◽

Lobaria Pulmonaria ◽

Anti Inflammatory ◽

Endogenous Antioxidant ◽

Inflammatory Effect

Abstract. Paw edema volume reduction is a useful marker in determining the anti-inflammatory effect of drugs and plant extracts in carrageenan-induced acute inflammation. In this study, the anti-inflammatory effect of Lobaria pulmonaria (LP) and Parmelia caperata (PC), two lichen species, was examined in carrageenan-induced mouse paw edema test. Compared to the controls in carrageenan-induced inflammation (n = 5/group), our results showed that pretreatment by single oral doses with PC extract (50–500 mg/kg) gives better results than LP extract (50–500 mg/kg) in terms of anti-edematous activity, as after 4 h of carrageenan subplantar injection, paw edema formation was inhibited at 82–99% by PC while at 35–49% by LP. The higher anti-inflammatory effect of PC, at all doses, was also observed on the time-course of carrageenan-induced paw edema, displaying profile closely similar to that obtained with diclofenac (25 mg/kg), an anti-inflammatory drug reference (all p < 0.001). Both LP and PC, at all doses, significantly ameliorated liver catalase (CAT) activity (all p < 0.05). However, superoxide dismutase (SOD) activity, glutathione peroxidase (GPx) activity and glutathione (GSH) levels were found increased in liver of PC- compared to LP-carrageenan-injected mice. Our findings demonstrated on one hand higher preventive effects of PC compared to LP in a mouse carrageenan-induced inflammatory model and suggested, on the other hand, that anti-inflammatory effects elicited by the two lichens were closely associated with the amelioration in the endogenous antioxidant status of liver.

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