Induction of a T-Helper 1 (Th1) Immune Response in Mice by an Extract from thePleurotus eryngii(Eringi) Mushroom

Synthetic oligodeoxynucleotides (ODN) that contain unmethylated CpG motifs (CpG ODN) induce macrophages to secrete IL-12, which induces interferon (IFN)-γ secretion by natural killer (NK) cells. Since these cytokines can induce T helper 1 (Th1) differentiation, we examined the effects of coadministered CpG ODN on the differentiation of Th responses to hen egg lysozyme (HEL). In both BALB/c (Th2-biased) and B10.D2 (Th1-biased) mice, immunization with HEL in incomplete Freund's adjuvant (IFA) resulted in Th2-dominated immune responses characterized by HEL-specific secretion of IL-5 but not IFN-γ. In contrast, immunization with IFA-HEL plus CpG ODN switched the immune response to a Th1-dominated cytokine pattern, with high levels of HEL-specific IFN-γ secretion and decreased HEL-specific IL-5 production. IFA-HEL plus CpG ODN also induced anti-HEL IgG2a (a Th1-associated isotype), which was not induced by IFA-HEL alone. Control non–CpG ODN did not induce IFN-γ or IgG2a, excepting lesser increases in B10.D2 (Th1-biased) mice. Thus, CpG ODN provide a signal to switch on Th1-dominated responses to coadministered antigen and are potential adjuvants for human vaccines to elicit protective Th1 immunity.

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Lactobacillus fermentum CJL-112 protects mice against influenza virus infection by activating T-helper 1 and eliciting a protective immune response

International Immunopharmacology ◽

10.1016/j.intimp.2013.10.020 ◽

2014 ◽

Vol 18 (1) ◽

pp. 50-54 ◽

Cited By ~ 13

Author(s):

Jung-Min Yeo ◽

Hyun-Jeong Lee ◽

Jae-Won Kim ◽

Joong-Bok Lee ◽

Seung-Yong Park ◽

...

Keyword(s):

Immune Response ◽

Influenza Virus ◽

Virus Infection ◽

Influenza Virus Infection ◽

Protective Immune Response ◽

Lactobacillus Fermentum ◽

T Helper ◽

T Helper 1

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Mycobacterium tuberculosisProtein Rv3841 Activates Dendritic Cells and Contributes to a T Helper 1 Immune Response

Journal of Immunology Research ◽

10.1155/2018/3525302 ◽

2018 ◽

Vol 2018 ◽

pp. 1-13 ◽

Cited By ~ 7

Author(s):

Seunga Choi ◽

Han-Gyu Choi ◽

Ki-Won Shin ◽

Yong Woo Back ◽

Hye-Soo Park ◽

...

Keyword(s):

Immune Response ◽

T Cells ◽

Dendritic Cells ◽

Protective Efficacy ◽

Mitogen Activated Protein Kinase ◽

T Helper ◽

T Helper 1 ◽

Cell Immunity ◽

Mitogen Activated Protein ◽

Mycobacterial Growth

The attenuated vaccineMycobacterium bovisBCG (Bacille Calmette Guerin) has limited protective efficacy against TB. The development of more effective TB vaccines has focused on the mycobacterial antigens that cause strong T helper 1 (Th1) responses. Mtb protein Rv3841 (bacterioferritin B; BfrB) is known to play a crucial role in the growth of Mtb. Nonetheless, it is unclear whether Rv3841 can induce protective immunity against Mtb. Here, we studied the action of Rv3841 in maturation of dendritic cells (DCs) and its engagement in the development of T-cell immunity. We found that Rv3841 functionally activated DCs by upregulating costimulatory molecules and increased secretion of proinflammatory cytokines. Activation of DCs by Rv3841 was mediated by Toll-like receptor 4 (TLR4), followed by triggering of mitogen-activated protein kinase and nuclear factor-κB signaling pathways. In addition, Rv3841-matured DCs effectively proliferated and polarized Th1 immune response of naïve CD4+and CD8+T-cells. Moreover, Rv3841 specifically caused the expansion of CD4+CD44highCD62LlowT-cells from Mtb-infected mice; besides, the T-cells activated by Rv3841-matured DCs inhibited intracellular mycobacterial growth. Our data suggest that Rv3841 induces DC maturation and protective immune responses, a finding that may provide candidate of effective TB vaccines.

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