scholarly journals Induction of Interferon-Gamma (IFN-γ) and T Helper 1 (Th1) Immune Response by Bitter Gourd Extract

2005 ◽  
Vol 67 (5) ◽  
pp. 521-524 ◽  
Author(s):  
Kazunori IKE ◽  
Yuko UCHIDA ◽  
Tomohiko NAKAMURA ◽  
Soichi IMAI
Keyword(s):  
Immune Response ◽  
Interferon Gamma ◽  
Bitter Gourd ◽  
T Helper ◽  
T Helper 1 ◽  
Ifn Gamma ◽  
Th1 Immune Response

676 FINE SPECIFICITY OF ANTI-NUP62 T-HELPER 1 CELL (TH1) IMMUNE RESPONSE IN PRIMARY BILIARY CIRRHOSIS

2009 ◽  
Vol 50 ◽  
pp. S248 ◽  
Author(s):  
F. Meda ◽  
M.S. Longhi ◽  
D.P. Bogdanos ◽  
P. Invernizzi ◽  
Y. Ma ◽  
...  
Keyword(s):  
Immune Response ◽  
Primary Biliary Cirrhosis ◽  
Biliary Cirrhosis ◽  
T Helper ◽  
T Helper 1 ◽  
Fine Specificity ◽  
Th1 Immune Response

scholarly journals Induction of a T-Helper 1 (Th1) Immune Response in Mice by an Extract from thePleurotus eryngii(Eringi) Mushroom

2012 ◽  
Vol 15 (12) ◽  
pp. 1124-1128 ◽  
Author(s):  
Kazunori Ike ◽  
Natsuko Kameyama ◽  
Akira Ito ◽  
Soichi Imai
Keyword(s):  
Immune Response ◽  
T Helper ◽  
T Helper 1 ◽  
Th1 Immune Response

scholarly journals Reversibility of T helper 1 and 2 populations is lost after long-term stimulation.

1996 ◽  
Vol 183 (3) ◽  
pp. 901-913 ◽  
Author(s):  
E Murphy ◽  
K Shibuya ◽  
N Hosken ◽  
P Openshaw ◽  
V Maino ◽  
...  
Keyword(s):  
Immune Response ◽  
Single Cells ◽  
Interleukin 4 ◽  
Th2 Cells ◽  
Th1 Cells ◽  
T Helper ◽  
T Helper 1 ◽  
Ifn Gamma ◽  
Th1 And Th2

Commitment of T helper 1 (Th1) or Th2 populations developing during an immune response to a pathogen, or an inappropriate immune response to an allergen or autoantigen, may determine the difference between health and chronic disease. We show that strongly polarized Th1 and Th2 populations assessed by immunoassay are heterogeneous using flow cytometry to detect single cells producing interferon gamma (IFN-gamma) and interleukin 4 (IL-4). Th1 populations arising after 1 wk of stimulation in IL-12 plus anti-IL-4 antibodies could convert to Th2 cells when restimulated in IL-4. Th2 populations resulting from stimulation for 1 wk in IL-4 could give rise to Th1 cells upon restimulation in IL-12 plus anti-IL-4. In contrast, the cytokine profiles of long-term Th1 and Th2 populations arising originally from repeated stimulation in IL-12 or IL-4 appeared more homogeneous and were not reversible, although IL-4 dramatically reduced the number of IFN-gamma-producing Th1 cells. This may explain previous reports that Th1 cells can be converted to Th2 cells.

scholarly journals Interleukin 12 induces stable priming for interferon gamma (IFN-gamma) production during differentiation of human T helper (Th) cells and transient IFN-gamma production in established Th2 cell clones.

1994 ◽  
Vol 179 (4) ◽  
pp. 1273-1283 ◽  
Author(s):  
R Manetti ◽  
F Gerosa ◽  
M G Giudizi ◽  
R Biagiotti ◽  
P Parronchi ◽  
...  
Keyword(s):  
T Cells ◽  
Interferon Gamma ◽  
Specific Antigen ◽  
Th2 Cells ◽  
Interleukin 12 ◽  
T Helper ◽  
Ifn Gamma ◽  
Selection Of

Interleukin 12 (IL-12) facilitates the generation of a T helper type 1 (Th1) response, with high interferon gamma (IFN-gamma) production, while inhibiting the generation of IL-4-producing Th2 cells in polyclonal cultures of both human and murine T cells and in vivo in the mouse. In this study, we analyzed the effect of IL-12, present during cloning of human T cells, on the cytokine profile of the clones. The culture system used allows growth of clones from virtually every T cell, and thus excludes the possibility that selection of precommitted Th cell precursors plays a role in determining characteristics of the clones. IL-12 present during the cloning procedures endowed both CD4+ and CD8+ clones with the ability to produce IFN-gamma at levels severalfold higher than those observed in clones generated in the absence of IL-12. This priming was stable because the high levels of IFN-gamma production were maintained when the clones were cultured in the absence of IL-12 for 11 d. The CD4+ and some of the CD8+ clones produced variable amounts of IL-4. Unlike IFN-gamma, IL-4 production was not significantly different in clones generated in the presence or absence of IL-12. These data suggest that IL-12 primes the clone progenitors, inducing their differentiation to high IFN-gamma-producing clones. The suppression of IL-4-producing cells observed in polyclonally generated T cells in vivo and in vitro in the presence of IL-12 is not observed in this clonal model, suggesting that the suppression depends more on positive selection of non-IL-4-producing cells than on differentiation of individual clones. However, antigen-specific established Th2 clones that were unable to produce IFN-gamma with any other inducer did produce IFN-gamma at low but significant levels when stimulated with IL-12 in combination with specific antigen or insoluble anti-CD3 antibodies. This induction of IFN-gamma gene expression was transient, because culture of the established clones with IL-12 for up to 1 wk did not convert them into IFN-gamma producers when stimulated in the absence of IL-12. These results suggest that Th clones respond to IL-12 treatment either with a stable priming for IFN-gamma production or with only a transient low level expression of the IFN-gamma gene, depending on their stage of differentiation.

scholarly journals Can levamisole upregulate the equine cell‐mediated macrophage (M1) dendritic cell (DC1) T‐helper 1 (CD4 Th1) T‐cytotoxic (CD8) immune response in vitro?

2019 ◽  
Vol 33 (2) ◽  
pp. 889-896 ◽  
Author(s):  
Sharon Witonsky ◽  
Virginia Buechner‐Maxwell ◽  
Amy Santonastasto ◽  
Robert Pleasant ◽  
Stephen Werre ◽  
...  
Keyword(s):  
Immune Response ◽  
Dendritic Cell ◽  
T Helper ◽  
T Helper 1 ◽  
Immune Response In Vitro

scholarly journals Pengaruh Simvastatin Terhadap Kadar Proliferasi Limfosit Mencit Balb/C yang Diinduce Sepsis dengan LPS

2013 ◽  
Vol 5 (3) ◽  
pp. 193
Author(s):  
Made Ryan Kharmayani ◽  
Haris Lutfi ◽  
Danu Soesilowati
Keyword(s):  
Interferon Gamma ◽  
Adhesion Molecule ◽  
Intercellular Adhesion Molecule ◽  
T Helper ◽  
Intercellular Adhesion Molecule 1 ◽  
T Helper 1 ◽  
Mhc Ii ◽  
Leukocyte Function ◽  
Post Test ◽  
Coa Reductase

Latar Belakang : Statin, inhibitor 3-hidroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase merupakan agen yang paling efektif dalam menurunkan lipid dan mempunyai efek pleiotrofik yaitu anti inflamatori dan immunomodulatori. Statin juga memodifikasi interaksi interseluler dan kemotaksis seluler pada sistem imun serta berpotensi mempengaruhi limfosit T dengan cara menghambat iinteraksi antara adhesi molekul seluler leukocyte function-associated antigen-1 (LFA-1) dan intercellular adhesion molecule-1 (ICAM-1), juga menurunkan interferon gamma (IFN -ɣ) yang berperan dalam ekspresi class II major histocompatibilty complex (MHC II) pada antigen precenting cells (APC) dan merupakan proses penting dalam aktivasi sel T. Penurunan ekspresi MHC II berakibat pada inhibisi aktivasi CD 4 limfosit, sehingga mengakibatkan penurunan diferensiasi T helper-1 (Th1) dan pelepasan sitokin proinflamasi juga menurun.Tujuan : Membuktikan efek simvastatin dosis bertingkat peroral pada mencit yang diberi LPS intraperitoneal terhadap penurunan kadar proliferasi limfosit.Metode : Penelitian eksperimental laboratorik dengan desain randomized post test only controlled group pada 20 ekor mencit Balb/c yang disuntik lipopolisakarida 10 mg/KgBB intraperitoneal dan simvastatin dosis 0,03 mg, 0,06 mg dan 0,12 mg peroral. Mencit dibagi menjadi 4 kelompok secara random, yaitu K1 sebagai control,  K2 yang mendapat simvastatin 0,03 mg, K3 yang mendapat simvastatin 0,06 mg dan K4 yang mendapat simvastatin 0,12 mg. Pemeriksaan limfosit diambil dari kultur limpa setelah 72 jam pemberian simvastatin. Uji statistik yang digunakan adalah parametrik ANOVA dan dilanjutkan PosterioriHasil : Kadar rerata limfosit kelompok K1 (1,546 ± 0,106), K2 (0,541 ± 0,046), K3 (0,471 ± 0,013) dan K4 (0,553 ± 0,02). Terdapat penurunan kadar limfosit secara signifikan pada kelompok K2, K3 dan K4 dibanding K1 dengan p <0,05. Tidak terdapat perbedaan bermakna antara kadar limfosit kelompok K2 dengan kelompok K3 dan K4 ( p>0,05) tetapi didapatkan perbedaan bermakna antara kelompok K3 dibandingkan kelompok K4 ( p<0,05).Simpulan : Simvastatin secara signifikan menurunkan kadar proliferasi limfosit pada mencit yang diberi LPS intraperitoneal. Dosis 0,06 mg memiliki efek menekan kadar proliferasi limfosit paling besar.

scholarly journals CpG Oligodeoxynucleotides Act as Adjuvants that Switch on T Helper 1 (Th1) Immunity

1997 ◽  
Vol 186 (10) ◽  
pp. 1623-1631 ◽  
Author(s):  
Rose S. Chu ◽  
Oleg S. Targoni ◽  
Arthur M. Krieg ◽  
Paul V. Lehmann ◽  
Clifford V. Harding
Keyword(s):  
Immune Response ◽  
Immune Responses ◽  
Cpg Odn ◽  
T Helper ◽  
T Helper 1 ◽  
Cpg Oligodeoxynucleotides ◽  
Cytokine Pattern ◽  
Incomplete Freund’S Adjuvant ◽  
Ifn Γ ◽  
Hen Egg

Synthetic oligodeoxynucleotides (ODN) that contain unmethylated CpG motifs (CpG ODN) induce macrophages to secrete IL-12, which induces interferon (IFN)-γ secretion by natural killer (NK) cells. Since these cytokines can induce T helper 1 (Th1) differentiation, we examined the effects of coadministered CpG ODN on the differentiation of Th responses to hen egg lysozyme (HEL). In both BALB/c (Th2-biased) and B10.D2 (Th1-biased) mice, immunization with HEL in incomplete Freund's adjuvant (IFA) resulted in Th2-dominated immune responses characterized by HEL-specific secretion of IL-5 but not IFN-γ. In contrast, immunization with IFA-HEL plus CpG ODN switched the immune response to a Th1-dominated cytokine pattern, with high levels of HEL-specific IFN-γ secretion and decreased HEL-specific IL-5 production. IFA-HEL plus CpG ODN also induced anti-HEL IgG2a (a Th1-associated isotype), which was not induced by IFA-HEL alone. Control non–CpG ODN did not induce IFN-γ or IgG2a, excepting lesser increases in B10.D2 (Th1-biased) mice. Thus, CpG ODN provide a signal to switch on Th1-dominated responses to coadministered antigen and are potential adjuvants for human vaccines to elicit protective Th1 immunity.

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