Effect of Low-Level Laser Therapy on Mast Cells in Second-Degree Burns in Rats

Low-level-laser therapy (LLLT) is an effective complementary treatment, especially for anti-inflammation and wound healing in which dermis or mucus mast cells (MCs) are involved. In periphery, MCs crosstalk with neurons via purinergic signals and participate in various physiological and pathophysiological processes. Whether extracellular ATP, an important purine in purinergic signaling, of MCs and neurons could be modulated by irradiation remains unknown. In this study, effects of red-laser irradiation on extracellular ATP content of MCs and dorsal root ganglia (DRG) neurons were investigated and underlying mechanisms were exploredin vitro. Our results show that irradiation led to elevation of extracellular ATP level in the human mast cell line HMC-1 in a dose-dependent manner, which was accompanied by elevation of intracellular ATP content, an indicator for ATP synthesis, together with [Ca2+]ielevation, a trigger signal for exocytotic ATP release. In contrast to MCs, irradiation attenuated the extracellular ATP content of neurons, which could be abolished by ARL 67156, a nonspecific ecto-ATPases inhibitor. Our results suggest that irradiation potentiates extracellular ATP of MCs by promoting ATP synthesis and release and attenuates extracellular ATP of neurons by upregulating ecto-ATPase activity. The opposite responses of these two cell types indicate complex mechanisms underlying LLLT.

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Comparative Efficacy of Low level Laser Therapy And Pulsed Ultrasound in the Management of Second - Degree Contusion

Nigerian Journal of Medical Rehabilitation ◽

10.34058/njmr.v11i2.15 ◽

2007 ◽

Author(s):

O Ayanniyi ◽

O B Ogunkunle ◽

C E Mbada

Keyword(s):

Laser Therapy ◽

Low Level Laser Therapy ◽

Comparative Efficacy ◽

Pulsed Ultrasound ◽

Low Level ◽

Low Level Laser ◽

Level Laser ◽

Group A ◽

Group B ◽

Second Degree

Low level LASER therapy (LLLT) and pulsed ultrasound have been shown to relieve pain, promote healing and tissue repair in sport injuries. The objective of this study was to investigate the comparative efficacy of LLLT and pulsed ultrasound in the management of second - degree contusion in sportsmen. This is a 2 group pre-test – post-test quasi – experimental study involving 20 sportsmen, ten were in Group A (LLLT) and ten in Group B (P-US). They were aged between 19 and 30 years with the mean age 24.9 ± 3.28 years and 27.4 ± 3.75 years for the Group A and Group B respectively. Treatment consisted of six sessions, in a period of 2 weeks. Data collected on each participants included age, weight, height, site of injury, pain intensity, swelling, calf girth and range of motion. The result of the study revealed decrease in pain for both LLLT and pulsed ultrasound (p0.05). It was concluded that both therapies are equally effective in the management of pain resulting from second – degree contusion in sportsmen. KEYWORDS: Low level LASER, pulsed ultrasound, second – degree contusions.

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