Methodological Analysis of Immunocytochemical Screening for Disseminated Epithelial Tumor Cells in Bone Marrow

1994 ◽  
Vol 3 (3) ◽  
pp. 165-173 ◽  
Author(s):  
KLAUS PANTEL ◽  
GÜNTER SCHLIMOK ◽  
MATTHIAS ANGSTWURM ◽  
DOROTHEA WECKERMANN ◽  
WERNER SCHMAUS ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Tumor Cells ◽  
Epithelial Tumor ◽  
Epithelial Tumor Cells

scholarly journals Occult epithelial tumor cells detected in bone marrow by an enzyme immunoassay specific for cytokeratin 19

1997 ◽  
Vol 70 (4) ◽  
pp. 396-400 ◽  
Author(s):  
Wilma Höchtlen-Vollmar ◽  
Rudolf Gruber ◽  
Heinz Bodenmüller ◽  
Erich Felber ◽  
Fritz Lindemann ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Enzyme Immunoassay ◽  
Tumor Cells ◽  
Cytokeratin 19 ◽  
Epithelial Tumor ◽  
Epithelial Tumor Cells

Disseminated Epithelial Tumor Cells in Bone Marrow of Patients with Esophageal Cancer: Detection and Prognostic Significance

1996 ◽  
Vol 20 (5) ◽  
pp. 567-573 ◽  
Author(s):  
Stefan Thorban ◽  
Jürgen D. Roder ◽  
Hjalmar Nekarda ◽  
Armin Funk ◽  
Klaus Pantel ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Esophageal Cancer ◽  
Tumor Cells ◽  
Cancer Detection ◽  
Prognostic Significance ◽  
Epithelial Tumor ◽  
Epithelial Tumor Cells

Epithelial tumor cells in bone marrow of patients with colorectal cancer: immunocytochemical detection, phenotypic characterization, and prognostic significance.

1990 ◽  
Vol 8 (5) ◽  
pp. 831-837 ◽  
Author(s):  
G Schlimok ◽  
I Funke ◽  
B Bock ◽  
B Schweiberer ◽  
J Witte ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Tumor Cells ◽  
Prognostic Significance ◽  
Phenotypic Characterization ◽  
Staining Procedure ◽  
Regional Lymph Nodes ◽  
Epithelial Tumor ◽  
Hla Class I Antigens ◽  
Primary Surgery ◽  
Epithelial Tumor Cells

A monoclonal antibody (mAb) directed against the cytokeratin (CK) polypeptide no. 18 specifically expressed in cells derived from simple epithelia was used to detect epithelial tumor cells in bone marrow aspirates. Of 156 patients with colorectal carcinoma, 42 presented with cells at the time of primary surgery. The incidence of positive findings varied considerably with the size and the localization of the primary tumor, the involvement of regional lymph nodes, and the presence of clinically manifest metastases. Applying a sensitive double-staining procedure, we could demonstrate that epithelial cells in bone marrow showed a heterogeneic expression of receptors for epidermal growth factor (EGF-R) and transferrin (Tf-R) as well as of the proliferation-associated Ki67 antigen. Also human leukocyte antigen (HLA) class I antigens differed widely in their expression on the CK-positive cells. Clinical follow-up studies on 85 patients showed a significantly higher relapse rate in patients presenting with CK-positive cells in their bone marrow at the time of primary surgery. Twenty-three patients were monitored for the presence or absence of CK-positive cells in bone marrow over time. The majority of monitored patients (18 of 23) exhibited a constant pattern of immunocytochemical findings during the time of observation. Thus, the technique may be useful in identifying high-risk patients as well as in monitoring adjuvant therapeutic trials.

scholarly journals Syndecan-4 Promotes Epithelial Tumor Cells Spreading and Regulates the Turnover of PKCα Activity under Mechanical Stimulation on the Elastomeric Substrates

2015 ◽  
Vol 36 (4) ◽  
pp. 1291-1304 ◽  
Author(s):  
Chun-Ping Huang ◽  
Chao-Min Cheng ◽  
Hong-Lin Su ◽  
Yi-Wen Lin
Keyword(s):  
Mechanical Stress ◽  
Tumor Cells ◽  
Focal Adhesion ◽  
Mechanical Stimulation ◽  
Binding Motif ◽  
Epithelial Tumor ◽  
Cell Contractility ◽  
Myosin Light Chain 2 ◽  
Epithelial Tumor Cells ◽  
Time Courses

Background: Heparan sulfate proteoglycans (HSPGs) at the cell surface play an important role in cell adhesion, spreading, formation of focal adhesion complexes (FACs), and sensing mechanical stress. Syndecans are members of the HSPGs family and are highly expressed in various tumor cells. Syndecan-4 (SDC4) is a unique member of syndecans that activates protein kinase C alpha (PKCα). However, syndecan-4 in tumor cells development is not clear when receiving mechanical stress. Aims: Here we investigate the role of syndecan-4 in tumor cells spreading and its downstream kinases under mechanical stimulation. Methods: Epithelial tumor cells were seeded onto elastomeric polydimethylsiloxane (PDMS) membranes coated with poly-L-lysine (Pl), fibronectin (Fn), or anti-SDC4 antibody and stretched with a modified pressure-driven cell-stretching (PreCS) device. Results: When cells received mechanical stimulation, engagement of syndecan-4 promoted the phosphorylation of focal adhesion kinase (FAK) at tyrosine 397 and PKCα at serine 657. Furthermore, we analyzed the cell contractility marker—myosin light chain 2 (MLC2) in 30 min time courses. The levels of phosphorylated MLC2 at serine19 were augmented through ligations of syndecan-4 but not integrin binding motif (RGD) at 10 min mechanical stimulation and were suppressed at 30 min and this phenomenon was associated with the activity of PKCα. Conclusion: Our data demonstrate that syndecan-4 is essential for transmitting the mechanotransduction signals via activation of PKCα and is important for tumor cells spreading, assembly of actin cytoskeleton and cell contractility.

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