High-Efficient Transfection of Human Embryonic Stem Cells by Single-Cell Plating and Starvation

2016 ◽  
Vol 25 (6) ◽  
pp. 477-491 ◽  
Author(s):  
Hui Liu ◽  
Caiping Ren ◽  
Bin Zhu ◽  
Lei Wang ◽  
Weidong Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Single Cell ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
High Efficient ◽  
Cell Plating

scholarly journals Identification and Single-Cell Functional Characterization of an Endodermally Biased Pluripotent Substate in Human Embryonic Stem Cells

2018 ◽  
Vol 10 (6) ◽  
pp. 1895-1907 ◽  
Author(s):  
Thomas F. Allison ◽  
Andrew J.H. Smith ◽  
Konstantinos Anastassiadis ◽  
Jackie Sloane-Stanley ◽  
Veronica Biga ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Single Cell ◽  
Human Embryonic Stem Cells ◽  
Functional Characterization ◽  
Embryonic Stem

scholarly journals Up-scaling single cell-inoculated suspension culture of human embryonic stem cells

2010 ◽  
Vol 4 (3) ◽  
pp. 165-179 ◽  
Author(s):  
Harmeet Singh ◽  
Pamela Mok ◽  
Thavamalar Balakrishnan ◽  
Siti Norfiza Binte Rahmat ◽  
Robert Zweigerdt
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Suspension Culture ◽  
Single Cell ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Up Scaling

scholarly journals Derivation of notochordal cells from human embryonic stem cells reveals unique regulatory networks by single cell‐transcriptomics

2019 ◽  
Vol 235 (6) ◽  
pp. 5241-5255 ◽  
Author(s):  
Martha E. Diaz‐Hernandez ◽  
Nazir M. Khan ◽  
Camila M. Trochez ◽  
Tim Yoon ◽  
Peter Maye ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Single Cell ◽  
Human Embryonic Stem Cells ◽  
Regulatory Networks ◽  
Embryonic Stem ◽  
Notochordal Cells

scholarly journals Temporal Analysis of Genome Alterations Induced by Single-Cell Passaging in Human Embryonic Stem Cells

2015 ◽  
Vol 24 (5) ◽  
pp. 653-662 ◽  
Author(s):  
Qiang Bai ◽  
Jean-Marie Ramirez ◽  
Fabienne Becker ◽  
Véronique Pantesco ◽  
Thierry Lavabre-Bertrand ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Single Cell ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Temporal Analysis

Fluorescence-Activated Single Cell Sorting of Human Embryonic Stem Cells

2006 ◽  
Vol 8 (3) ◽  
pp. 225-234 ◽  
Author(s):  
Z. Hewitt ◽  
N.R. Forsyth ◽  
M. Waterfall ◽  
D. Wojtacha ◽  
A.J. Thomson ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Single Cell ◽  
Human Embryonic Stem Cells ◽  
Cell Sorting ◽  
Embryonic Stem

scholarly journals BMP-treated human embryonic stem cells transcriptionally resemble amnion cells in the monkey embryo

Biology Open ◽  
2021 ◽  
Author(s):  
Sapna Chhabra ◽  
Aryeh Warmflash
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Single Cell ◽  
Human Embryonic Stem Cells ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Embryonic Stem ◽  
Cell Types ◽  
Human Embryos ◽  
Early Human

Human embryonic stem cells (hESCs) possess an immense potential to generate clinically relevant cell types and unveil mechanisms underlying early human development. However, using hESCs for discovery or translation requires accurately identifying differentiated cell types through comparison with their in vivo counterparts. Here, we set out to determine the identity of much debated BMP-treated hESCs by comparing their transcriptome to recently published single cell transcriptomic data from early human embryos (Xiang et al., 2019). Our analyses reveal several discrepancies in the published human embryo dataset, including misclassification of putative amnion, intermediate and inner cell mass cells. These misclassifications primarily resulted from similarities in pseudogene expression, highlighting the need to carefully consider gene lists when making comparisons between cell types. In the absence of a relevant human dataset, we utilized the recently published single cell transcriptome of the early post implantation monkey embryo to discern the identity of BMP-treated hESCs. Our results suggest that BMP-treated hESCs are transcriptionally more similar to amnion cells than trophectoderm cells in the monkey embryo. Together with prior studies, this result indicates that hESCs possess a unique ability to form mature trophectoderm subtypes via an amnion-like transcriptional state.

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