Downregulation of Extracellular Matrix-Related Gene Clusters during Osteogenic Differentiation of Human Bone Marrow- and Adipose Tissue-Derived Stromal Cells

The relationships between donor age and gender and initial isolation yield and the osteogenic potentials of human bone marrow stromal cells (hBMSCs) have not been clearly elucidated. The authors investigated whether isolation yields and the osteogenic differentiation potentials of hBMSCs are indeed dependent on donor age or gender. Fresh bone marrow was aspirated from iliac crest of 72 donors (mean age 54.1 years; range, 23-84 years; 39 men and 33 women) undergoing total hip arthroplasty. Numbers of mononuclear cells, numbers of colony forming unit-fibroblasts (CFU-Fs) and alkaline phosphatase (ALP)-positive CFU-Fs, and numbers of BMSCs after isolation culture were not found to be significantly dependent on donor age or gender. Moreover, no significant age- or gender-related differences were observed in terms of the proliferation activities, ALP activities, and calcium contents of BMSCs during in vitro osteogenic differentiation. The data obtained from 72 human donors revealed no significant age- or genderrelated differences among hBMSCs in terms of isolation yields, proliferation activities, and osteogenic potentials.

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Comparison of mesenchymal stromal cells from human bone marrow and adipose tissue for the treatment of spinal cord injury

Cytotherapy ◽

10.1016/j.jcyt.2012.11.015 ◽

2013 ◽

Vol 15 (4) ◽

pp. 434-448 ◽

Cited By ~ 73

Author(s):

Zhilai Zhou ◽

Yinhai Chen ◽

Hui Zhang ◽

Shaoxiong Min ◽

Bo Yu ◽

...

Keyword(s):

Spinal Cord ◽

Bone Marrow ◽

Spinal Cord Injury ◽

Adipose Tissue ◽

Mesenchymal Stromal Cells ◽

Stromal Cells ◽

Human Bone ◽

Human Bone Marrow ◽

Cord Injury

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Stromal cell-associated erythropoiesis

Blood ◽

10.1182/blood.v67.5.1418.1418 ◽

1986 ◽

Vol 67 (5) ◽

pp. 1418-1426 ◽

Cited By ~ 16

Author(s):

S Tsai ◽

CA Sieff ◽

DG Nathan

Keyword(s):

Bone Marrow ◽

Extracellular Matrix ◽

Stromal Cells ◽

Stromal Cell ◽

Culture Medium ◽

Serum Proteins ◽

Human Bone ◽

Human Bone Marrow ◽

Erythroid Progenitors ◽

Culture Dish

Abstract A novel cover slip-transfer culture system was designed to study the functional roles of stromal cells in hemopoiesis, particularly erythropoiesis. Human bone marrow stromal cell colonies were allowed to develop on small glass cover slips in liquid medium. The cover slips, along with the stromal cell colonies and progenitors attached to them were then transferred to a new tissue culture dish and overlaid with methylcellulose culture medium. No exogenous colony-stimulating factors except erythropoietin were supplied. Large erythroid bursts, comprising multiple subcolonies, developed on the stromal cells. In order to determine if stromal fibroblasts together with erythropoietin and serum proteins could support erythroid development, human bone marrow cells depleted of monocytes, macrophages, and T lymphocytes were allowed to adhere to monolayers of a homogeneous fibroblastoid human stromal cell strain ST-1 grown on cover slips. The cover slips were then washed to remove nonadherent cells, transferred to a new culture dish, and overlaid with methylcellulose culture medium containing fetal calf serum and erythropoietin. In this modified system as well, primitive erythroid progenitors migrated extensively on and within the stroma to form huge colonies of hemoglobinized erythroblasts that proceeded to enucleate. Our results indicate that (1) ST-1 cells together with serum proteins and erythropoietin can support the development of large erythroid bursts; (2) erythroid progenitors and precursors adhere to and migrate on and within the extracellular matrix elaborated by ST-1 cells; (3) erythroid progenitors are more adherent to the ST-1 cells or the extracellular matrix than are the more mature cells and possibly the myeloid progenitors.

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Remodeling of Three-Dimensional Collagen I Matrices by Human Bone Marrow Stromal Cells during Osteogenic Differentiation In Vitro

ACS Applied Bio Materials ◽

10.1021/acsabm.0c00856 ◽

2020 ◽

Vol 3 (10) ◽

pp. 6967-6978 ◽

Cited By ~ 2

Author(s):

Sarah Vogel ◽

Franziska Ullm ◽

Claudia Damaris Müller ◽

Tilo Pompe ◽

Ute Hempel

Keyword(s):

Bone Marrow ◽

Osteogenic Differentiation ◽

Stromal Cells ◽

Bone Marrow Stromal Cells ◽

Three Dimensional ◽

Marrow Stromal Cells ◽

Human Bone ◽

Collagen I ◽

Human Bone Marrow

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Human Bone Marrow Stromal Cells: A Reliable, Challenging Tool forIn VitroOsteogenesis and Bone Tissue Engineering Approaches

Stem Cells International ◽

10.1155/2016/7842191 ◽

2016 ◽

Vol 2016 ◽

pp. 1-14 ◽

Cited By ~ 15

Author(s):

Ute Hempel ◽

Katrin Müller ◽

Carolin Preissler ◽

Carolin Noack ◽

Sabine Boxberger ◽

...

Keyword(s):

Bone Marrow ◽

Osteogenic Differentiation ◽

Stromal Cells ◽

Bone Marrow Stromal Cells ◽

Marrow Stromal Cells ◽

Human Bone ◽

Human Bone Marrow ◽

Peroxisome Proliferator ◽

Differentiation Potential

Adult human bone marrow stromal cells (hBMSC) are important for many scientific purposes because of their multipotency, availability, and relatively easy handling. They are frequently used to study osteogenesisin vitro. Most commonly, hBMSC are isolated from bone marrow aspirates collected in clinical routine and cultured under the “aspect plastic adherence” without any further selection. Owing to the random donor population, they show a broad heterogeneity. Here, the osteogenic differentiation potential of 531 hBMSC was analyzed. The data were supplied to correlation analysis involving donor age, gender, and body mass index. hBMSC preparations were characterized as follows: (a) how many passages the osteogenic characteristics are stable in and (b) the influence of supplements and culture duration on osteogenic parameters (tissue nonspecific alkaline phosphatase (TNAP), octamer binding transcription factor 4, core-binding factor alpha-1, parathyroid hormone receptor, bone gla protein, and peroxisome proliferator-activated proteinγ). The results show that no strong prediction could be made from donor data to the osteogenic differentiation potential; only the ratio of induced TNAP to endogenous TNAP could be a reliable criterion. The results give evidence that hBMSC cultures are stable until passage 7 without substantial loss of differentiation potential and that established differentiation protocols lead to osteoblast-like cells but not to fully authentic osteoblasts.

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Osteogenic Differentiation of Human Bone Marrow Stromal Cells Cultured on Hydroxyl Carbonate Apatite Layer Preformed on Poly (ε-aprolactone)–Organosiloxane Nano-Hybrid Materials

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.309-311.573 ◽

2006 ◽

Vol 309-311 ◽

pp. 573-576

Author(s):

Jeong Joon Yoo ◽

Hee Joong Kim ◽

Sang Hoon Rhee

Keyword(s):

Bone Marrow ◽

Osteogenic Differentiation ◽

Hybrid Material ◽

Stromal Cells ◽

Bone Marrow Stromal Cells ◽

Apatite Layer ◽

Marrow Stromal Cells ◽

Human Bone ◽

Human Bone Marrow ◽

Carbonate Apatite

Previous study showed that the novel poly(ε-caprolactone)–organosiloxane nano-hybrid material (SiOPCL) had bioactivity, biodegradability, and mechanical properties comparable to those of human cancellous bone. In this study, hydroxyl carbonate apatite layer (HCA) was preformed on the surface of SiOPCL, which mimicked the events likely to occur in vivo, and cellular behaviors of human bone marrow stromal cells (hBMSCs) were investigated during the osteogenic differentiation on its surface (SiOPCL/HCA). Initial attachment, proliferation, and ALP activities of hBMSCs were comparable to those on tissue culture plates (TCPs), while the calcium content in the cell layer showed significantly higher value. It means that this novel bioactive nano-hybrid material is likely to be a promising candidate for bone grafting materials because of good hBMSCs responses as well as apatite forming ability in the simulated body fluid.

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