Prevention of Osmotic Injury to Human Umbilical Vein Endothelial Cells for Biopreservation: A First Step Toward Biobanking of Endothelial Cells for Vascular Tissue Engineering

2016 ◽  
Vol 22 (3) ◽  
pp. 270-279 ◽  
Author(s):  
Dan Niu ◽  
Gang Zhao ◽  
Xiaoli Liu ◽  
Ping Zhou ◽  
Yunxia Cao
Keyword(s):  
Tissue Engineering ◽  
Endothelial Cells ◽  
Vascular Tissue ◽  
Umbilical Vein ◽  
Vascular Tissue Engineering ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells

Bio-Electrospraying of Human Umbilical Vein Endothelial Cells with a Customized Multi-Hole Spinneret

2016 ◽  
Vol 705 ◽  
pp. 291-296 ◽  
Author(s):  
Ting Zhang ◽  
Yuan Yuan Liu ◽  
Hong Chen Yu ◽  
Shuai Li ◽  
Hai Ping Chen ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Endothelial Cells ◽  
Cell Morphology ◽  
Trypan Blue ◽  
Umbilical Vein ◽  
Engineering Applications ◽  
Human Umbilical Vein ◽  
Sulforhodamine B ◽  
Umbilical Vein Endothelial Cells

Bio-electrospraying (BES) is becoming an attractive tool for the delivery of cells into scaffolds for tissue engineering applications. In this study, we aimed to electrospray human umbilical vein endothelial cells (HUVECs) and improve the efficiency of BES by designing a new customized multi-hole spinneret. We demonstrated that the multi-hole spinneret could produce continuous and stable jets during BES, and the efficiency was increased by 5–7 times. Morphological observations, trypan blue and sulforhodamine B assays revealed that the HUVECs electrosprayed using the multi-hole spinneret remained viable and proliferated at a rate similar to that of the controls. Thus, the new multi-hole nozzle can considerably improve output for BES without affecting cell morphology, viability, and proliferation.

scholarly journals Advantages of human umbilical vein scaffolds derived from cesarean section vs. vaginal delivery for vascular tissue engineering

Biomaterials ◽  
2008 ◽  
Vol 29 (8) ◽  
pp. 1075-1084 ◽  
Author(s):  
Markus Hoenicka ◽  
Volker R. Jacobs ◽  
Georgine Huber ◽  
Franz-Xaver Schmid ◽  
Dietrich E. Birnbaum
Keyword(s):  
Tissue Engineering ◽  
Cesarean Section ◽  
Vaginal Delivery ◽  
Vascular Tissue ◽  
Umbilical Vein ◽  
Vascular Tissue Engineering ◽  
Human Umbilical Vein

Evaluation of decellularized human umbilical vein (HUV) for vascular tissue engineering - comparison with endothelium-denuded HUV

2012 ◽  
Vol 9 (1) ◽  
pp. 13-23 ◽  
Author(s):  
Silvia Mangold ◽  
Siegfried Schrammel ◽  
Georgine Huber ◽  
Markus Niemeyer ◽  
Christof Schmid ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Vascular Tissue ◽  
Umbilical Vein ◽  
Vascular Tissue Engineering ◽  
Human Umbilical Vein

The Effect of Diameter of Electronspun PGA Scaffold for Biological Behaviour of Human Umbilical Vein Endothelial Cells

2007 ◽  
Vol 342-343 ◽  
pp. 237-240 ◽  
Author(s):  
Furong Tian ◽  
Hossein Hossinkhani ◽  
Yoshiro Yokoyama ◽  
Giovani Gomes Estrada ◽  
Hisatoshi Kobayashi
Keyword(s):  
Endothelial Cells ◽  
Cell Adhesion ◽  
Glycolic Acid ◽  
Vascular Tissue ◽  
Umbilical Vein ◽  
Silicone Membrane ◽  
Biological Behaviour ◽  
Human Umbilical Vein ◽  
Scaffold Materials ◽  
Umbilical Vein Endothelial Cells

The objective of this work was to investigate cell adhesion, Poly Glycolic Acid (PGA) and PGA/ collagen nano-fibers on the silicone membrane. PGA with the weight-mixing ration of 40% was fabricated through the electronspun technique. The behaviors of Human Umbilical Vein Endothelial cells on these scaffolds are evaluated. The highest cell adhesion was observed in the PGA/collagen fibers with the diameter of 500 nm. This study indicates the effect of nano-fibers on the Human Umbilical Vein Endothelial cells for better understanding of interactions of cells with scaffold materials. Such information will have important implications for implantable vascular tissue engineering constructs.

Sign in / Sign up

Export Citation Format

Share Document