Generation and Evaluation of Recombinant Baculovirus Coexpressing GP5 and M Proteins of Porcine Reproductive and Respiratory Syndrome Virus Type 1

2021 ◽  
Vol 34 (10) ◽  
pp. 697-707
Author(s):  
Wang Xu ◽  
Shouwen Du ◽  
Tiyuan Li ◽  
Shipin Wu ◽  
Ningyi Jin ◽  
...  
Keyword(s):  
Virus Type ◽  
Recombinant Baculovirus ◽  
Respiratory Syndrome Virus ◽  
M Proteins

scholarly journals Human immunodeficiency virus type 1 subtype C Gag virus-like particle boost substantially improves the immune response to a subtype C gag DNA vaccine in mice

2004 ◽  
Vol 85 (2) ◽  
pp. 409-413 ◽  
Author(s):  
Ann Jaffray ◽  
Enid Shephard ◽  
Joanne van Harmelen ◽  
Carolyn Williamson ◽  
Anna-Lise Williamson ◽  
...  
Keyword(s):  
Immune Response ◽  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Recombinant Baculovirus ◽  
Vaccination Strategy ◽  
Subtype C ◽  
Immunodeficiency Virus ◽  
Hiv 1

Human immunodeficiency virus type 1 (HIV-1) subtype C is the predominant HIV in southern Africa, and is the target of a number of recent vaccine candidates. It has been proposed that a heterologous prime/boost vaccination strategy may result in stronger, broader and more prolonged immune responses. Since HIV-1 Gag Pr55 polyprotein can assemble into virus-like particles (VLPs) which have been shown to induce a strong cellular immune response in animals, we showed that a typical southern African subtype C Pr55 protein expressed in insect cells via recombinant baculovirus could form VLPs. We then used the baculovirus-produced VLPs as a boost to a subtype C HIV-1 gag DNA prime vaccination in mice. This study shows that a low dose of HIV-1 subtype C Gag VLPs can significantly boost the immune response to a single subtype C gag DNA inoculation in mice. These results suggest a possible vaccination regimen for humans.

Herpes simplex Virus Type 1 DNase: Functional Analysis of the Enzyme Expressed by Recombinant Baculovirus

Intervirology ◽  
1998 ◽  
Vol 41 (2-3) ◽  
pp. 110-119 ◽  
Author(s):  
Elke Kehm ◽  
Mehmet-Ali Göksu ◽  
Stefanie Bayer ◽  
Charles W. Knopf
Keyword(s):  
Functional Analysis ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Recombinant Baculovirus ◽  
Simplex Virus

EXPRESSION OF VP2 PROTEIN OF RAT MINUTE VIRUS TYPE 1 (RMV-1) IN RECOMBINANT BACULOVIRUS AND ITS APPLICATION TO DIAGNOSIS OF RMV-1 INFECTION

2014 ◽  
Vol 40 (01) ◽  
pp. 21-27
Author(s):  
Cho-Hua Wan ◽  
David J. Pintel ◽  
Lela K. Riley
Keyword(s):  
Capsid Protein ◽  
Virus Type ◽  
Recombinant Baculovirus ◽  
Structural Protein ◽  
Minute Virus Of Mice ◽  
Vp2 Protein ◽  
Laboratory Rats ◽  
Minute Virus

Rat minute virus type 1 (RMV-1) was identified in laboratory rats. In this study, an ELISA using a recombinant capsid protein (rVP2) of RMV-1 was developed to diagnose RMV infections. The developed RMV-1 rVP2 ELISA exhibited a sensitivity of 97.5% and a specificity of 100%, and was more sensitive and specific in detecting RMV-1 infection than the ELISA using a recombinant minute virus of mice non-structural protein as antigen.

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