scholarly journals Anniversary of the discovery/isolation of the yeast centromere by Clarke and Carbon

2015 ◽  
Vol 26 (9) ◽  
pp. 1575-1577 ◽  
Author(s):  
Kerry Bloom
Keyword(s):  
Fission Yeast ◽  
Dna Sequence ◽  
Budding Yeast ◽  
Sequence Specificity ◽  
Molecular Tools ◽  
Dna Sequence Specificity ◽  
Artificial Chromosomes

The first centromere was isolated 35 years ago by Louise Clarke and John Carbon from budding yeast. They embarked on their journey with rudimentary molecular tools (by today's standards) and little knowledge of the structure of a chromosome, much less the nature of a centromere. Their discovery opened up a new field, as centromeres have now been isolated from fungi and numerous plants and animals, including mammals. Budding yeast and several other fungi have small centromeres with short, well-defined sequences, known as point centromeres, whereas regional centromeres span several kilobases up to megabases and do not seem to have DNA sequence specificity. Centromeres are at the heart of artificial chromosomes, and we have seen the birth of synthetic centromeres in budding and fission yeast and mammals. The diversity in centromeres throughout phylogeny belie conserved functions that are only beginning to be understood.

scholarly journals The DNA sequence specificity of stimulation of DNA polymerases by factor D.

1987 ◽  
Vol 262 (18) ◽  
pp. 8868-8874 ◽  
Author(s):  
M Fry ◽  
R Sharf ◽  
P Weisman-Shomer ◽  
P C Evers ◽  
L A Loeb
Keyword(s):  
Dna Sequence ◽  
Dna Polymerases ◽  
Sequence Specificity ◽  
Dna Sequence Specificity ◽  
Stimulation Of

Thermodynamic and Kinetic Basis for the Relaxed DNA Sequence Specificity of “Promiscuous” Mutant EcoRI Endonucleases

2005 ◽  
Vol 348 (2) ◽  
pp. 307-324 ◽  
Author(s):  
Paul J. Sapienza ◽  
Crystal A. dela Torre ◽  
William H. McCoy ◽  
Samyukta V. Jana ◽  
Linda Jen-Jacobson
Keyword(s):  
Dna Sequence ◽  
Sequence Specificity ◽  
Dna Sequence Specificity

Reaction of the antitumor antibiotic CC-1065 with DNA: structure of a DNA adduct with DNA sequence specificity

Science ◽  
1984 ◽  
Vol 226 (4676) ◽  
pp. 843-844 ◽  
Author(s):  
L. Hurley ◽  
V. Reynolds ◽  
D. Swenson ◽  
G. Petzold ◽  
T. Scahill
Keyword(s):  
Dna Sequence ◽  
Dna Structure ◽  
Dna Adduct ◽  
Antitumor Antibiotic ◽  
Sequence Specificity ◽  
Dna Sequence Specificity

scholarly journals A plant DNA-binding protein that recognizes 5-methylcytosine residues.

1989 ◽  
Vol 9 (3) ◽  
pp. 1351-1356 ◽  
Author(s):  
D L Zhang ◽  
K C Ehrlich ◽  
P C Supakar ◽  
M Ehrlich
Keyword(s):  
Dna Binding ◽  
Dna Sequence ◽  
Base Pair ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Sequence Specificity ◽  
Dna Sequence Specificity ◽  
Plant Dna ◽  
Related Family ◽  
Nuclear Extracts

A novel, 5-methylcytosine-specific, DNA-binding protein, DBP-m, has been identified in nuclear extracts of peas. DBP-m specifically recognizes 5-methylcytosine residues in DNA without appreciable DNA sequence specificity, unlike a mammalian DNA-binding protein (MDBP), which recognizes 5-methylcytosine residues but only in a related family of 14-base-pair sequences.

scholarly journals Evolution of DNA replication origin specification and gene silencing mechanisms

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Y. Hu ◽  
A. Tareen ◽  
Y-J. Sheu ◽  
W. T. Ireland ◽  
C. Speck ◽  
...  
Keyword(s):  
Dna Replication ◽  
Gene Silencing ◽  
Dna Sequence ◽  
Origin Recognition Complex ◽  
Separate Analysis ◽  
Sequence Specificity ◽  
Replication Origins ◽  
Origin Firing ◽  
Dna Sequence Specificity ◽  
Α Helix

Abstract DNA replication in eukaryotic cells initiates from replication origins that bind the Origin Recognition Complex (ORC). Origin establishment requires well-defined DNA sequence motifs in Saccharomyces cerevisiae and some other budding yeasts, but most eukaryotes lack sequence-specific origins. A 3.9 Å structure of S. cerevisiae ORC-Cdc6-Cdt1-Mcm2-7 (OCCM) bound to origin DNA revealed that a loop within Orc2 inserts into a DNA minor groove and an α-helix within Orc4 inserts into a DNA major groove. Using a massively parallel origin selection assay coupled with a custom mutual-information-based modeling approach, and a separate analysis of whole-genome replication profiling, here we show that the Orc4 α-helix contributes to the DNA sequence-specificity of origins in S. cerevisiae and Orc4 α-helix mutations change genome-wide origin firing patterns. The DNA sequence specificity of replication origins, mediated by the Orc4 α-helix, has co-evolved with the gain of ORC-Sir4-mediated gene silencing and the loss of RNA interference.

scholarly journals Role for first zinc finger of WT1 in DNA sequence specificity: Denys–Drash syndrome-associated WT1 mutant in ZF1 enhances affinity for a subset of WT1 binding sites

2017 ◽  
Vol 46 (8) ◽  
pp. 3864-3877 ◽  
Author(s):  
Dongxue Wang ◽  
John R Horton ◽  
Yu Zheng ◽  
Robert M Blumenthal ◽  
Xing Zhang ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Zinc Finger ◽  
Binding Sites ◽  
Sequence Specificity ◽  
Dna Sequence Specificity ◽  
Drash Syndrome

Dna Sequence Specificity of Antitumor Agents: Oncogenes as possible targets for cancer therapy

1988 ◽  
Vol 27 (5) ◽  
pp. 503-510 ◽  
Author(s):  
J. A. Hartley ◽  
J. W. Lown ◽  
W. B. Mattes ◽  
K. W. Kohn
Keyword(s):  
Cancer Therapy ◽  
Dna Sequence ◽  
Antitumor Agents ◽  
Sequence Specificity ◽  
Dna Sequence Specificity
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