Determination of Serum Zinc Concentrations in Normal Adult Subjects by Atomic Absorption Spectrophotometry

Abstract Of two sensitive complexometric reagents for the colorimetry of serum zinc that we investigated, one, 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol (Br-PADAP), was found to be a potentially useful compound for trace-metal determinations. It has a high molar absorptivity (120 000 L mol-1 cm-1) but is not convenient to use because it is not very soluble in water. The other reagent, a related pyridylazo compound, is 2-(5-bromo-2-pyridylazo)-5-(N-n-propyl-N-3-sulfopropylamino)phenol (5-BR-PAPS). It seems better suited for use in routine zinc determinations because, besides being water soluble, it has a higher molar absorptivity, 130 000 L mol-1 cm-1. Results by the proposed method developed with 5-Br-PAPS correlated well with those by atomic absorption spectrophotometry. The between-run CV for control sera was less than 5%; the within-run CV (same controls) was less than 4%.

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DETERMINATION OF SERUM TRACE ELEMENTS MAGNESIUM, COPPER, ZINC, AND SELENIUM IN ASTHMATIC PATIENTS BY ATOMIC ABSORPTION SPECTROPHOTOMETRY

Journal of Al-Nahrain University-Science ◽

10.22401/jnus.13.1.03 ◽

2010 ◽

Vol 13 (1) ◽

pp. 20-25

Author(s):

Nahla A. Al-Assaf ◽

Keyword(s):

Trace Elements ◽

Atomic Absorption ◽

Atomic Absorption Spectrophotometry ◽

Asthmatic Patients ◽

Absorption Spectrophotometry ◽

Copper Zinc ◽

Serum Trace Elements

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Determination of Minor Nutrients in Fertilizers by Atomic Absorption Spectrophotometry: Second Collaborative Study

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/48.6.1100 ◽

1965 ◽

Vol 48 (6) ◽

pp. 1100-1103

Author(s):

C H Mcbride

Keyword(s):

Atomic Absorption ◽

Collaborative Study ◽

Atomic Absorption Spectrophotometry ◽

Absorption Method ◽

Atomic Absorption Method ◽

Absorption Spectrophotometry ◽

Minor Nutrients

Abstract The atomic absorption method studied last year was re-examined and extended to include calcium and sodium. The procedures were submitted to 16 collaborators for determination of Ca, Cu, Fe, Mg, Mn, Na, and Zn. Results for Ca and Na were discouraging; further study is recommended.

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Comparison of Methods for Determining Biuret in Urea

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/62.1.153 ◽

1979 ◽

Vol 62 (1) ◽

pp. 153-159

Author(s):

Luis F Corominas ◽

Victor M Boy ◽

Manuel Guijosa

Keyword(s):

Atomic Absorption ◽

Spectrophotometric Determination ◽

Atomic Absorption Spectrophotometry ◽

International Organization ◽

Comparison Of Methods ◽

Definite Advantage ◽

Absorption Spectrophotometry ◽

Alternative Procedures ◽

Aoac Method

Abstract The official first action AOAC method for the spectrophotometric determination of biuret in urea, 2.072—2.074, was compared with official first action AOAC method 2.C01-2.C03 (atomic absorption spectrophotometry), 2 simplified versions of 2.072-2.074, and modified versions of 2 alternative procedures of the International Organization for Standardization. Three synthetic urea samples (0.3, 1.4, and 3.0% biuret) and 1 commercial urea sample (1.0% biuret) were analyzed. The methods proved to be equivalent and none showed a definite advantage over 2.072-2.074. The purification of biuret and the interference by ammonia are also discussed.

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