Utility of Image Processing and Analysis (IPA) for Diagnosis of Very Short Segment Hirschsprung Disease (vsHD)

Purpose Quantification of calretinin-stained mucosal nerve fibers by image processing and analysis (IPA) may objectively define the transition zone (TZ) of Hirschsprung disease (HD). We tested the utility of IPA as an adjunctive tool in HD. Materials and Methods Calretinin immunostain was performed on 15 HD pull-through specimens, and multiple images were captured from the proximal aganglionic zone, TZ, and probable normal zone (NZ). Pixel count (PC), defined as the percentage of brown-stained pixels in the mucosa, was quantified and plotted against distance from the rectal distal end. To validate the method, PCs from 45 images were compared with three-tiered visual scoring by five pathologists. Results were correlated against pertinent variables, which were retrieved from the clinical record. Results The PC gradually increased in the TZ toward the proximal resection margin in 10/13 (77%) cases. The PC variation in the probable NZ and around the circumference was substantial by the coefficient of variation. The mean PC of images with a visual score of 1 was less than scores of 2 and 3 by all five (100%) pathologists (p < 0.01). One patient had possible TZ pull-through that was clinically confirmed. Conclusion While the mucosal calretinin staining gradually increases in the TZ, for now, the boundaries of the TZ cannot be accurately defined by mucosal biopsies given the substantial variation of staining around the circumference at the same distance and in the NZ. However, the IPA technique does provide a continuous variable and warrants further utility in HD studies.

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Transition Zone in Total Colonic Aganglionosis and Colorectal Hirschsprung Disease Shows a Similar Trend of Mucosal Innervation: Image Processing and Analysis (IPA) Study

American Journal of Clinical Pathology ◽

10.1093/ajcp/aqy090.089 ◽

2018 ◽

Vol 150 (suppl_1) ◽

pp. S36-S37

Author(s):

Natallia Sheuka ◽

Fernanda Cordeiro-Rudnisky ◽

Christine Whyte ◽

Sangtae Ahn ◽

Ann Boguniewicz ◽

...

Keyword(s):

Image Processing ◽

Transition Zone ◽

Hirschsprung Disease ◽

Total Colonic Aganglionosis ◽

Image Processing And Analysis ◽

Mucosal Innervation

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Functional outcomes of patients with short-segment Hirschsprung disease after transanal endorectal pull-through

BMC Gastroenterology ◽

10.1186/s12876-021-01668-x ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Gunadi ◽

Gabriele Ivana ◽

Desyifa Annisa Mursalin ◽

Ririd Tri Pitaka ◽

Muhammad Wildan Zain ◽

...

Keyword(s):

Functional Outcomes ◽

Hirschsprung Disease ◽

Treatment Method ◽

Strongly Correlated ◽

Short Segment ◽

Multicenter Studies ◽

Operative Complications ◽

Pull Through ◽

Larger Sample ◽

Post Operative Complications

Abstract Background Transanal endorectal pull-through (TEPT) is considered the most preferable treatment method for Hirschsprung disease (HSCR) since it is less invasive and has fewer morbidities than transabdominal pull-through. Here, functional outcomes in short-segment HSCR patients after TEPT were assessed and associated with the prognostic factors. Methods Krickenbeck classification was used to assess the functional outcomes in patients with HSCR after TEPT surgery at our institution from 2012 to 2020. Results Fifty patients were involved in this study. Voluntary bowel movement (VBM) was achieved in 82% of subjects. Nine (18%) subjects had soiling grade 1, while two (4%) and two (4%) patients suffered constipation that was manageable with diet and laxative agents, respectively. Patients who underwent TEPT at ≥ 4 years old tended to have soiling more than patients who underwent TEPT at < 4 years old (OR = 16.47 [95% CI 0.9–301.61]; p = 0.06), whereas patients with post-operative complications had 10.5-fold higher risk for constipation than patients without post-operative complications (p = 0.037; 95% CI 1.15–95.92). Multivariate analysis showed male sex was significantly associated with VBM (OR = 9.25 [95% CI 1.34–63.77]; p = 0.024), while post-operative complications were strongly correlated with constipation (OR = 10 [95% CI 1.09–91.44]; p = 0.04). Conclusions The functional outcomes of HSCR patients after TEPT in our institution are considered relatively good. Moreover, the VBM, soiling, and constipation risk after TEPT might be affected by sex, age at TEPT performed, and post-operative complications, respectively, while the age at TEPT performed might not be associated with functional outcomes. Further multicenter studies with a larger sample size are necessary to clarify and confirm our findings.

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Comparative Analysis of Modified Laparoscopic Swenson and Laparoscopic Soave Procedure for Short-Segment Hirschsprung Disease in Children

European Journal of Pediatric Surgery ◽

10.1055/s-0034-1384647 ◽

2014 ◽

Vol 25 (05) ◽

pp. 430-434

Author(s):

Yaohao Wu ◽

LeXiang Zeng ◽

Jie Zhang ◽

Jiajia Zhou ◽

Ronglin Qiu ◽

...

Keyword(s):

Comparative Analysis ◽

Hirschsprung Disease ◽

Short Segment

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Validation of a new image analysis procedure for quantifying filamentous bacteria in activated sludge

Water Science & Technology ◽

10.2166/wst.2014.296 ◽

2014 ◽

Vol 70 (6) ◽

pp. 955-963 ◽

Cited By ~ 4

Author(s):

Ewa Liwarska-Bizukojc ◽

Marcin Bizukojc ◽

Olga Andrzejczak

Keyword(s):

Image Processing ◽

Image Analysis ◽

Activated Sludge ◽

Analysis Procedure ◽

Automated Image Analysis ◽

Filamentous Bacteria ◽

Image Processing And Analysis ◽

Manual Counting ◽

Analysis Application ◽

Activated Sludge Systems

Quantification of filamentous bacteria in activated sludge systems can be made by manual counting under a microscope or by the application of various automated image analysis procedures. The latter has been significantly developed in the last two decades. In this work a new method based upon automated image analysis techniques was elaborated and presented. It consisted of three stages: (a) Neisser staining, (b) grabbing of microscopic images, and (c) digital image processing and analysis. This automated image analysis procedure possessed the features of novelty. It simultaneously delivered data about aggregates and filaments in an individual calculation routine, which is seldom met in the procedures described in the literature so far. What is more important, the macroprogram performing image processing and calculation of morphological parameters was written in the same software which was used for grabbing of images. Previously published procedures required using two different types of software, one for image grabbing and another one for image processing and analysis. Application of this new procedure for the quantification of filamentous bacteria in the full-scale as well as laboratory activated sludge systems proved that it was simple, fast and delivered reliable results.

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Intelligent Life System Modelling, Image Processing and Analysis

10.1007/978-981-16-7207-1 ◽

2021 ◽

Keyword(s):

Image Processing ◽

System Modelling ◽

Image Processing And Analysis ◽

Intelligent Life

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Microdeformations in Sands by Digital Image Processing and Analysis

Transportation Research Record Journal of the Transportation Research Board ◽

10.1177/0361198196154800105 ◽

1996 ◽

Vol 1548 (1) ◽

pp. 31-37 ◽

Cited By ~ 5

Author(s):

Scott A. Raschke ◽

Roman D. Hryciw ◽

Gregory W. Donohoe

Keyword(s):

Image Processing ◽

Digital Image Processing ◽

Digital Image ◽

Small Scale ◽

Two Dimensional ◽

Localized Deformation ◽

Displacement Fields ◽

Particulate Media ◽

Image Processing And Analysis ◽

Video Images

Laboratory experiments are typically performed on particulate media to study stress-deformation behavior and to verify or calibrate computer models from controlled or measured boundary stresses and displacements. However, such data do not permit the formation of shear bands, displacement fields within flowing granular media, and other small-scale localized deformation phenomena to be identified. Described are two semiautomated computer vision techniques for accurately determining the two-dimensional displacement field in granular soils from video images obtained through a transparent planar viewing window. The techniques described are applicable for studying the behavior of particulate media under plane strain and certain axisymmetric test conditions. Digital image processing and analysis routines are used in two different computer programs, Tracker and Tracer, Tracker uses a graphical user interface that allows individual particles to be selected and tracked through a sequence of digital video images. A contrast edge detection algorithm delineates the two-dimensional projected boundaries of particles. The location of the centroid of each particle selected for tracking is determined from the boundary to quantify the trajectory of each particle. Tracer maps the trace or trajectory of specially dyed fluorescent particles in a sequence of video frames. A thresholding technique segments individual particle trajectories. Together, Tracker and Tracer provide a set of tools for identifying small-scale displacement fields in particulate assemblies deforming under either quasi-static or rapid loading (such as gravity flow).

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