Uniform Labeling

1971 ◽  
Vol 28 (5) ◽  
pp. 323-324
Author(s):  
Niles B. Ross
Keyword(s):  
Uniform Labeling

scholarly journals Association of a protease with polytene chromosomes of Drosophila melanogaster.

1980 ◽  
Vol 87 (2) ◽  
pp. 415-419 ◽  
Author(s):  
J Cavagnaro ◽  
D A Pierce ◽  
J C Lucchesi ◽  
C B Chae
Keyword(s):  
Acetic Acid ◽  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Protease Inhibitors ◽  
Polytene Chromosomes ◽  
Fixation Procedure ◽  
Apparent Effect ◽  
Chloromethyl Ketone ◽  
Diisopropyl Fluorophosphate ◽  
Uniform Labeling

Incubation of Drosophila salivary glands with radioactive diisopropyl fluorophosphate results in the uniform labeling of polytene chromosomes. Extensive labeling is seen only when chromosome squashes are prepared by a formaldehyde fixation procedure and not by standard acetic acid techniques. The labeling is inhibited in the presence of tosylphenylalanine chloromethyl ketone and phenylmethane sulfonylfluoride but not by tosyllysine chloromethyl ketone, suggesting that a chymotrypsin-like serine protease is associated with the chromosomes. Protease inhibitors show no apparent effect on heat-shock specific puffing.

scholarly journals Feature-rich covalent stains for super-resolution and cleared tissue fluorescence microscopy

2020 ◽  
Vol 6 (22) ◽  
pp. eaba4542 ◽  
Author(s):  
Chenyi Mao ◽  
Min Yen Lee ◽  
Jing-Ru Jhan ◽  
Aaron R. Halpern ◽  
Marcus A. Woodworth ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Fluorescent Dyes ◽  
Super Resolution ◽  
Fluorescent Labeling ◽  
Processing Conditions ◽  
Sample Processing ◽  
Tissue Fluorescence ◽  
Uniform Labeling ◽  
Tissue Microscopy

Fluorescence microscopy is a workhorse tool in biomedical imaging but often poses substantial challenges to practitioners in achieving bright or uniform labeling. In addition, while antibodies are effective specific labels, their reproducibility is often inconsistent, and they are difficult to use when staining thick specimens. We report the use of conventional, commercially available fluorescent dyes for rapid and intense covalent labeling of proteins and carbohydrates in super-resolution (expansion) microscopy and cleared tissue microscopy. This approach, which we refer to as Fluorescent Labeling of Abundant Reactive Entities (FLARE), produces simple and robust stains that are modern equivalents of classic small-molecule histology stains. It efficiently reveals a wealth of key landmarks in cells and tissues under different fixation or sample processing conditions and is compatible with immunolabeling of proteins and in situ hybridization labeling of nucleic acids.

A scanning electron microscope study of lectin receptors on neuroblastoma cells

1978 ◽  
Vol 36 (2) ◽  
pp. 302-303
Author(s):  
P. Maher ◽  
R.S. Molday
Keyword(s):  
Ricinus Communis ◽  
Neuroblastoma Cells ◽  
Fluorescent Light ◽  
Con A ◽  
Polymeric Microspheres ◽  
Lectin Receptors ◽  
Scanning Electron Microscope Study ◽  
Visual Markers ◽  
Uniform Labeling ◽  
Scanning Electron

The organization and redistribution of concanavalin A (Con A), Ricinus communis agglutinin I (RCA I) and wheat germ agglutinin (WGA) receptors on mouse neuroblastoma cells was analyzed using lectins conjugated to fluorescent polymeric microspheres as visual markers for scanning electron microscopy (SEM) and fluorescent light microscopys. Results indicate that all three lectins are internalized by these cells but the patterns of redistribution for Con A differ from those of RCA I and WGA.Labeling of prefixed undifferentiated and differentiated neuroblastoma cells indicated that Con A, WGA and RCA I receptors were densely and uniformly distributed over the cell surface and neurites. Cells treated with lectin for 5-10 minutes at 37°C, washed free of excess reagent and maintained for up to 15 minutes in buffer exhibited uniform labeling patterns.

Probing Molecular Interfaces Using 2D Magic-Angle-Spinning NMR on Protein Mixtures with Different Uniform Labeling

2004 ◽  
Vol 126 (45) ◽  
pp. 14746-14751 ◽  
Author(s):  
Manuel Etzkorn ◽  
Anja Böckmann ◽  
Adam Lange ◽  
Marc Baldus
Keyword(s):  
Magic Angle Spinning ◽  
Magic Angle ◽  
Uniform Labeling ◽  
Angle Spinning

scholarly journals NON-UNIFORM LABELING OF INSULIN AND RIBONUCLEASE SYNTHESIZED IN VITRO

1954 ◽  
Vol 211 (1) ◽  
pp. 367-374
Author(s):  
Martha Vaughan ◽  
Christian B. Anfinsen
Keyword(s):  
Uniform Labeling

Implication of Dispensing Cups in Dosing Errors and Pediatric Poisonings: A Report from the American Association of Poison Control Centers

1992 ◽  
Vol 26 (7-8) ◽  
pp. 917-918 ◽  
Author(s):  
Toby Litovitz
Keyword(s):  
American Association ◽  
Case Series ◽  
Poison Control ◽  
Poison Control Centers ◽  
Dosing Error ◽  
Liquid Medication ◽  
Poison Centers ◽  
Uniform Labeling ◽  
Design Case ◽  
Labeling System

OBJECTIVE: To characterize reports to poison centers involving liquid medication errors associated with the use of dispensing cups. design: Case series reported by 16 US poison centers over an eight-day period. SETTING: Calls to poison control centers, predominantly but not exclusively from homes. PATIENTS: Children and adults. RESULTS: Of 34 reported cases, most (79 percent) involved a two- to threefold dosing error, and most (94 percent) involved an error in a single dose of medication. Cough and cold preparations were implicated in 65 percent; acetaminophen elixirs in 18 percent. Three major causes of dosing errors were identified, including teaspoon/tablespoon confusion, assumption that the dispensing cup was the unit of measure, and assumption that the full dispensing cup was the actual dose. CONCLUSIONS: Dispensing cup markings should use a single unit of measure, and a uniform labeling system should be implemented. Teaspoon/tablespoon abbreviations should be avoided, and dispensing cup lettering should be more legible. Consumer education is essential to correct the misimpression that the full cup is always the recommended dose.

scholarly journals Ultrafast immunostaining of organ-scale tissues for scalable proteomic phenotyping

2019 ◽  
Author(s):  
Dae Hee Yun ◽  
Young-Gyun Park ◽  
Jae Hun Cho ◽  
Lee Kamentsky ◽  
Nicholas B. Evans ◽  
...  
Keyword(s):  
Animal Models ◽  
Three Dimensional ◽  
Chemical Transport ◽  
Cell Types ◽  
Spatial Context ◽  
Disease Models ◽  
Comprehensive Understanding ◽  
Wide Range ◽  
Unique Approach ◽  
Uniform Labeling

ABSTRACTStudying the function and dysfunction of complex biological systems necessitates comprehensive understanding of individual cells. Advancements in three-dimensional (3D) tissue processing and imaging modalities have enabled rapid visualization and phenotyping of cells in their spatial context. However, system-wide interrogation of individual cells within large intact tissue remains challenging, low throughput, and error-prone owing to the lack of robust labeling technologies. Here we introduce a rapid, versatile, and scalable method, eFLASH, that enables complete and uniform labeling of organ-scale tissue within one day. eFLASH dynamically modulates chemical transport and reaction kinetics to establish system-wide uniform labeling conditions throughout the day-long labeling period. This unique approach enables the same protocol to be compatible with a wide range of tissue types and probes, enabling combinatorial molecular phenotyping across different organs and species. We applied eFLASH to generate quantitative maps of various cell types in mouse brains. We also demonstrated multidimensional cell profiling in a marmoset brain block. We envision that eFLASH will spur holistic phenotyping of emerging animal models and disease models to help assess their functions and dysfunctions.

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