scholarly journals Extensive Protein Similarity of the Hybridizing Chickadees Parus atricapillus and P. carolinensis

The Auk ◽  
1986 ◽  
Vol 103 (4) ◽  
pp. 667-675 ◽  
Author(s):  
Michael J. Braun ◽  
Mark B. Robbins
Keyword(s):  
Contact Zone ◽  
Gel Electrophoresis ◽  
Isozyme Variation ◽  
Starch Gel Electrophoresis ◽  
Parus Atricapillus ◽  
Parus Gambeli ◽  
Starch Gel ◽  
Well Differentiated ◽  
Emerging Pattern ◽  
Electrophoresis Of Proteins

Abstract Starch gel electrophoresis of proteins was used to assess genetic differentiation and introgression across a contact zone between Parus atricapillus and P. carolinensis. Little or no differentiation was found at 35 presumed genetic loci, even between distantly allopatric population samples. Nei's (1978) genetic distance (D) was ≤0.001 for all comparisons. In contrast, Parus gambeli, another chickadee known to hybridize with atricapillus, was well differentiated at 3 loci (D ≈ 0.065). While the data suggest that atricapillus and carolinensis are closely related, they do not allow conclusions on the extent of introgression across the contact zone. The implications of these data are discussed in the light of the emerging pattern of isozyme variation in birds.

scholarly journals Starch-Gel Electrophoresis of Wheat Flour Proteins

1963 ◽  
Vol 16 (2) ◽  
pp. 342 ◽  
Author(s):  
Janet SD Graham
Keyword(s):  
Acetic Acid ◽  
Wheat Flour ◽  
Gel Electrophoresis ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Starch Gel Electrophoresis ◽  
Similar Protein ◽  
Starch Gel ◽  
Protein Components ◽  
Electrophoresis Of Proteins

An improved apparatus and procedures for starch-gel electrophoresis of proteins of wheat flour are described; highly reproducible separation of the protein components was achieved. By starch-gel electrophoresis it was shown that similar protein components occur in the extracts of wheat flour obtained with a variety of solvents; however, there were marked differences in the proportions of these components in various extracts. Several protein components were present in the fJ'actions separated by ion-exchange chromatography of' the proteins soluble in Bodium pyrophosphate and of those soluble in acetic acid; some fractions containeda number of similar protein components.

Isozyme variation in the fungus Atkinsonella hypoxylon within and among populations of its host grasses

1989 ◽  
Vol 67 (9) ◽  
pp. 2600-2607 ◽  
Author(s):  
Adrian Leuchtmann ◽  
Keith Clay
Keyword(s):  
Gel Electrophoresis ◽  
Gene Diversity ◽  
Host Population ◽  
Multilocus Genotype ◽  
Isozyme Variation ◽  
Starch Gel Electrophoresis ◽  
Gene Exchange ◽  
Multilocus Genotypes ◽  
Starch Gel ◽  
Atkinsonella Hypoxylon

Isozyme variation of 291 isolates of Atkinsonella hypoxylon (Clavicipitaceae, tribe Balansieae) from 24 populations of its four known host grasses (Danthonia compressa, Danthonia sericea, Danthonia spicata, and Stipa leucotricha) was examined using starch gel electrophoresis. In total, there were 20 distinct multilocus genotypes. Eleven out of 13 enzyme loci (84.6%) exhibited more than one allele (mean 2.8) per locus. Nei's total gene diversity (HT) within all isolates was 0.229. Between isolate samples from S. leucotricha and the three Danthonia hosts, Nei's genetic identity (I) ranged from 0.21 to 0.31 and among isolate samples from the three Danthonia species I ranged from 0.65 to 0.88, with isolates from D. spicata and D. compressa being most similar. Variation of A. hypoxylon occurred both within and among populations of D. spicata and D. compressa, where up to 53 isolates were sampled per host population. In contrast, all 20 isolates from S. leucotricha were identical, as were all 6 from D. sericea. A few isolates from D. spicata exhibited the same, unusual multilocus genotype with unique alleles at six different loci. The occurrence of several multilocus genotypes in isolates from the same ascostroma and the 1:1 segregation of genotypes among ascospores from a single ascus indicated gene exchange among sexually reproducing individuals, consistent with a heterothallic mating system for A. hypoxylon.

A revision of the species of Porphyra (Rhodophyta: Bangiales) occurring in British Columbia and adjacent waters

1992 ◽  
Vol 70 (10) ◽  
pp. 2066-2075 ◽  
Author(s):  
Sandra C. Lindstrom ◽  
Kathleen M. Cole
Keyword(s):  
British Columbia ◽  
New Species ◽  
North Pacific ◽  
Gel Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Southeast Alaska ◽  
Starch Gel ◽  
One New Species ◽  
New Distribution ◽  
Electrophoresis Of Proteins

Starch gel electrophoresis of proteins of Porphyra species occurring in British Columbia and nearby areas has provided new data on species identities. One new species is described, Porphyra kurogii (formerly identified as North Pacific P. purpurea), and its eastern Pacific distribution limit is extended from northern to southern British Columbia. Porphyra maculosa is recognized to be a taxonomic synonym of P. fucicola; new distribution records are provided. Porphyra cuneiformis is the correct name for specimens formerly identified as P. miniata in the area, and P. occidentalis is the correct name for most local specimens of P. "variegata." A key to the 21 species and 2 subspecies of Porphyra currently recognized in Oregon, Washington, British Columbia, and southeast Alaska is provided. The key includes the recently described P. mumfordii, P. fallax ssp. fallax, and P. fallax ssp. conwayae. Key words: isozymes, key, Porphyra, taxonomy.

Isozyme variation between species and formae speciales of the genus Puccinia

1981 ◽  
Vol 59 (12) ◽  
pp. 2628-2634 ◽  
Author(s):  
J. J. Burdon ◽  
D. R. Marshall
Keyword(s):  
Gel Electrophoresis ◽  
Soluble Proteins ◽  
Isozyme Variation ◽  
Starch Gel Electrophoresis ◽  
Banding Patterns ◽  
Formae Speciales ◽  
Starch Gel

Soluble proteins extracted from germinating urediniospores of 11 different species of Puccinia Pers. and from three formae spéciales of each of 3 of these species were subjected to starch gel electrophoresis and the resultant isozyme banding patterns compared.Isozyme patterns obtained for the 11 different species showed considerable differentiation between species, indicating that all species were distinct taxonomic entities. Patterns obtained for the formae speciales were, in general, much more similar and suggested relationships which agree with existing knowledge from taxonomic and fertility studies.

Fractionation of Plasminogen by Starch Gel Electrophoresis

1964 ◽  
Vol 12 (01) ◽  
pp. 126-136 ◽  
Author(s):  
Karl H. Slotta ◽  
J. D Gonzalez
Keyword(s):  
Gel Electrophoresis ◽  
Room Temperature ◽  
Broad Band ◽  
Caproic Acid ◽  
Starch Gel Electrophoresis ◽  
Full Activity ◽  
Veronal Buffer ◽  
Starch Gel ◽  
Alkaline Range

SummaryWhen urea or ε-amino caproic acid were used as solublizing agents for plasminogen in electrophoretic experiments, only one broad band of the proenzyme was obtained on acetate cellulose, in starch block, and in acrylamide gel. In starch gel electrophoresis, however, both forms of plasminogen – the native or euglobulin and Kline’s or Pseudoglobulin plasminogen – separated into six bands. These migrated toward the cathode at room temperature in borate or veronal buffer in the alkaline range and showed full activity in fibrinagar-streptokinase plates.

scholarly journals THE INHERITANCE OF ENZYME VARIANTS FOR TYROSINE AMINOTRANSFERASE, NADP-DEPENDENT MALATE DEHYDROGENASE, NADP-DEPENDENT ISOCITRATE DEHYDROGENASE, AND TETRAZOLIUM OXIDASE IN TETRAHYMENA PYRIFORMIS, SYNGEN 1

Genetics ◽  
1973 ◽  
Vol 74 (4) ◽  
pp. 595-603
Author(s):  
D Borden ◽  
E T Miller ◽  
D L Nanney ◽  
G S Whitt
Keyword(s):  
Detailed Analysis ◽  
Malate Dehydrogenase ◽  
Isocitrate Dehydrogenase ◽  
Gel Electrophoresis ◽  
Tetrahymena Pyriformis ◽  
Tyrosine Aminotransferase ◽  
Breeding Program ◽  
Starch Gel Electrophoresis ◽  
Enzyme Locus ◽  
Starch Gel

ABSTRACT The isozymic patterns of tyrosine aminotransferase, NADP malate dehydrogenase, NADP isocitrate dehydrogenase, and tetrazolium oxidase were examined by starch-gel electrophoresis in Tetrahymena pyriformis, syngen 1. The genetics of the alleles controlling these enzymes was studied through a breeding program. Each enzyme locus was shown to assort vegetatively, as do other loci in this organism. A detailed analysis of the assortment process for the tyrosine aminotransferase locus indicated that the rate of stabilization of heterozygotes into pure types was essentially identical to previously-reported rates for other loci.

Sign in / Sign up

Export Citation Format

Share Document