FCSlib: an open-source tool for fluorescence fluctuation spectroscopy analysis for mobility, number and molecular brightness in R

2020 ◽  
Author(s):  
R Pinto-Cámara ◽  
A Linares ◽  
D S Moreno-Gutiérrez ◽  
H O Hernández ◽  
J D Martínez-Reyes ◽  
...  
Keyword(s):  
Open Source ◽  
Fluorescence Correlation Spectroscopy ◽  
Pair Correlation ◽  
Correlation Spectroscopy ◽  
Supplementary Information ◽  
Spectroscopy Analysis ◽  
Fluorescence Fluctuation Spectroscopy ◽  
Fluorescence Correlation ◽  
Sample Data ◽  
Molecular Brightness

Abstract Summary FCSlib is an open-source R tool for fluorescence fluctuation spectroscopy data analysis. It encompasses techniques such as Fluorescence Correlation Spectroscopy, Number and Brightness, Pair Correlation Function and Pair Correlation of Molecular Brightness. Availability and implementation Source code available at https://cran.r-project.org/web/packages/FCSlib/ for Linux, Windows and macOS platforms. Sample data as well as a user's guide are available at https://github.com/FCSlib/FCSlib. Contact [email protected] Supplementary information Supplementary data are available at Bioinformatics online.

scholarly journals FRET at the Single Molecule Level using Molecular Brightness and Fluorescence Correlation Spectroscopy

2019 ◽  
Vol 116 (3) ◽  
pp. 567a ◽  
Author(s):  
Robert C. Miller ◽  
Rowan Simonet ◽  
Christin Libal ◽  
Cody Aplin ◽  
Anh Cong ◽  
...  
Keyword(s):  
Single Molecule ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Single Molecule Level ◽  
Fluorescence Correlation ◽  
Molecular Brightness

scholarly journals FCSlib: An Open-Source Tool for Fluorescence Fluctuation Spectroscopy Analysis for Mobility, Number, and Molecular Brightness in R

2021 ◽  
Vol 120 (3) ◽  
pp. 175a
Author(s):  
Alejandro Linares ◽  
Raúl Pinto-Cámara ◽  
David Moreno ◽  
Haydee Hernández ◽  
José Martínez ◽  
...  
Keyword(s):  
Open Source ◽  
Spectroscopy Analysis ◽  
Fluorescence Fluctuation Spectroscopy ◽  
Open Source Tool ◽  
Molecular Brightness

scholarly journals A Comprehensive Review of Fluorescence Correlation Spectroscopy

2021 ◽  
Vol 9 ◽  
Author(s):  
Lan Yu ◽  
Yunze Lei ◽  
Ying Ma ◽  
Min Liu ◽  
Juanjuan Zheng ◽  
...  
Keyword(s):  
Diffusion Coefficient ◽  
Fluorescence Correlation Spectroscopy ◽  
Pair Correlation ◽  
Time Correlation ◽  
Correlation Spectroscopy ◽  
Local Concentration ◽  
Fluorescence Correlation ◽  
Pros And Cons

Fluorescence correlation spectroscopy (FCS) is a powerful technique for quantification of molecular dynamics, and it has been widely applied in diverse fields, e.g., biomedicine, biophysics, and chemistry. By time-correlation of the fluorescence fluctuations induced by molecules diffusing through a focused light, FCS can quantitatively evaluate the concentration, diffusion coefficient, and interaction of the molecules in vitro or in vivo. In this review, the basic principle and implementation of FCS are introduced. Then, the advances of FCS variants are reviewed, covering dual-color FCCS, multi-focus FCS, pair correlation function (pCF), scanning FCS, focus-reduced FCS, SPIM-FCS, and inverse-FCS. Besides, the applications of FCS are demonstrated with the measurement of local concentration, hydrodynamic radius, diffusion coefficient, and the interaction of different molecules. Lastly, a discussion is given by summarizing the pros and cons of different FCS techniques, as well as the outlooks and perspectives of FCS.

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