scholarly journals Variant protein patterns in hepatomas and transformed liver cell lines as determined by high resolution two-dimensional gel electrophoresis (2DE)

1992 ◽  
Vol 13 (7) ◽  
pp. 1177-1183 ◽  
Author(s):  
Evelyn Zeindl-Eberhart ◽  
Hartmut M. Rabes
Keyword(s):  
High Resolution ◽  
Cell Lines ◽  
Liver Cell ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Protein Patterns ◽  
Two Dimensional Gel Electrophoresis ◽  
Variant Protein

Diversity of Glycoprotein Deficiencies in Glanzmann’s Thrombasthenia

1985 ◽  
Vol 54 (03) ◽  
pp. 626-629 ◽  
Author(s):  
M Meyer ◽  
F H Herrmann
Keyword(s):  
High Resolution ◽  
Gel Electrophoresis ◽  
Type Ii ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Structural Variant ◽  
Glanzmann’S Thrombasthenia ◽  
Crossed Immunoelectrophoresis ◽  
Glanzmann's Thrombasthenia ◽  
Platelet Proteins

SummaryThe platelet proteins of 9 thrombasthenic patients from 7 families were analysed by high resolution two-dimensional gel electrophoresis (HR-2DE) and crossed immunoelectrophoresis (CIE). In 7 patients both glycoproteins (GPs) IIb and Ilia were absent or reduced to roughly the same extent. In two related patients only a trace of GP Ilb-IIIa complex was detected in CIE, but HR-2DE revealed a glycopeptide in the position of GP Ilia in an amount comparable to type II thrombasthenia. This GP Ilia-like component was neither recognized normally by anti-GP Ilb-IIIa antibodies nor labeled by surface iodination. In unreduced-reduced two-dimensional gel electrophoresis two components were observed in the region of GP Ilia. The assumption of a structural variant of GP Ilia in the two related patients is discussed.

Technology: Methodology for High-Resolution Two-Dimensional Gel Electrophoresis

1987 ◽  
Vol 5 (2) ◽  
pp. 127-149 ◽  
Author(s):  
A Thomas Endler ◽  
Donald S. Young ◽  
Russell P. Tracy
Keyword(s):  
High Resolution ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

scholarly journals Demonstration of a third structurally distinct human Ia beta chain by two-dimensional gel electrophoresis.

1982 ◽  
Vol 156 (2) ◽  
pp. 652-657 ◽  
Author(s):  
R W Karr ◽  
C C Kannapell ◽  
J A Stein ◽  
T C Fuller ◽  
R J Duquesnoy ◽  
...  
Keyword(s):  
Cell Lines ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Beta Chain ◽  
Two Dimensional Gel Electrophoresis ◽  
Hla Dr

Previous studies have indicated that HLA-DR homozygous cell lines express two Ia alpha and Ia beta chains that combine to form at least two Ia molecules. This report demonstrates by two-dimensional gel electrophoresis the existence of a third structurally distinct human Ia beta chain on DR2 and DR5 cell lines. This suggests that at least five separate genes control the expression of Ia molecules on HLA-DR homozygous cell lines.

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