scholarly journals Effects of chronic nicotine on the autocrine regulation of pancreatic cancer cells and pancreatic duct epithelial cells by stimulatory and inhibitory neurotransmitters

2012 ◽  
Vol 33 (9) ◽  
pp. 1745-1753 ◽  
Author(s):  
M. H. Al-Wadei ◽  
H. A. N. Al-Wadei ◽  
H. M. Schuller
Keyword(s):  
Pancreatic Cancer ◽  
Epithelial Cells ◽  
Pancreatic Duct ◽  
Cancer Cells ◽  
Inhibitory Neurotransmitters ◽  
Chronic Nicotine ◽  
Pancreatic Cancer Cells ◽  
Autocrine Regulation

scholarly journals Activated Pancreatic Stellate Cells Enhance the Warburg Effect to Cause the Malignant Development in Chronic Pancreatitis

2021 ◽  
Author(s):  
Tao Ye ◽  
Shao Feng ◽  
Liu Zheng ◽  
Cai Ming ◽  
Meng Futao ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Chronic Pancreatitis ◽  
Epithelial Cells ◽  
Pancreatic Duct ◽  
Cancer Cells ◽  
Stellate Cells ◽  
Glycolytic Enzymes ◽  
Pancreatic Stellate Cells ◽  
Migration And Invasion ◽  
Pancreatic Cancer Cells

Abstract Background: Chronic pancreatitis (CP) is a precancerous condition associated with pancreatic ductal adenocarcinoma (PDAC), but its evolutionary mechanism is unclear. pancreatic stellate cells (PSCs) are closely related to the occurrence and development of CP and PDAC. We aimed to find out whether PSCs play a key role in this " inflammationcancer transition ". Methods: To evaluate the effect of activated pancreatic stellate cells on normal pancreatic duct epithelial cells and pancreatic cancer cells, pancreatic stellate cells isolated from human tissues were co-cultured with these two cells, respectively. Functional assays assessed the proliferation, migration, and invasion of these two cells. RT-qPCR and western blotting were used to detect the mRNA and protein expressions of glycolytic enzymes in these two cells. Lactate production and glucose utilization assays assessed the aerobic glycolysis level of these two cells. Immunohistochemistry was used to detect the expression of glycolytic enzymes and α-SMA, and the correlation between the two was analyzed in human tissues. Results: Our research found that co-culture with activated PSCs promoted the proliferation, migration and invasion of normal pancreatic duct epithelial cells and pancreatic cancer cells. At the same time, activated PSCs had a significant effect on the expression of the glycolytic enzymes PKM2 and LDHA in normal pancreatic duct epithelial cells and pancreatic cancer cells and increased lactic acid production and glucose consumption in these two cells. In vivo experiments showed that the expression of the glycolytic enzymes PKM2 and LDHA in pancreatic duct epithelial cells and the marker protein (α-SMA) of activated PSCs in the pancreatic duct peripancreatic interstitium were higher in pancreatic cancer tissues and chronic pancreatitis tissues than in normal pancreatic tissues in both animals and humans. In addition, analysis of human tissue specimens showed that there is a correlation between the expression of PKM2/LDHA and α-SMA. Conclusion: These findings indicate that activated PSCs play an important role in the development and progression of chronic pancreatitis into pancreatic cancer by regulating and promoting aerobic glycolysis. Our research provides a new theoretical basis for further understanding the mechanism of CP malignancy and the selection of targets for reversing CP malignancy.

scholarly journals Activated Pancreatic Stellate Cells Enhance the Warburg Effect to Cause the Malignant Development in Chronic Pancreatitis

2021 ◽  
Author(s):  
Tao Ye ◽  
Shao Feng ◽  
Liu Zheng ◽  
Cai Ming ◽  
Meng Futao ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Chronic Pancreatitis ◽  
Epithelial Cells ◽  
Pancreatic Duct ◽  
Cancer Cells ◽  
Stellate Cells ◽  
Monocarboxylate Transporter ◽  
Pancreatic Stellate Cells ◽  
Glucose Transporter 1 ◽  
Pancreatic Cancer Cells

Abstract Chronic pancreatitis (CP) is a precancerous condition associated with pancreatic ductal adenocarcinoma (PDAC), but its evolutionary mechanism is unclear. Pancreatic stellate cells (PSCs) are closely related to the occurrence and development of CP and PDAC, but it is not clear whether PSCs play a key role in this "inflammation-cancer transition". Our research found that co-culture with activated PSCs promoted the proliferation, migration and invasion of normal pancreatic duct epithelial cells and pancreatic cancer cells. At the same time, activated PSCs had a significant effect on the expression of the glycolysis markers (pyruvate kinase M2, lactate dehydrogenase A, glucose transporter 1, hexokinase-II and monocarboxylate transporter 4; PKM2, LDHA, GLUT1, HK2 and MCT4) in normal pancreatic duct epithelial cells and pancreatic cancer cells and increased lactic acid production and glucose consumption in these two cells. In vivo experiments showed that the expression of the glycolysis markers in pancreatic duct epithelial cells and the marker protein (α-SMA) of activated PSCs in the pancreatic duct peripancreatic interstitium were higher in pancreatic cancer tissues and chronic pancreatitis tissues than in normal pancreatic tissues in both animals and humans. In addition, analysis of human tissue specimens showed that there is a correlation between the expression of glycolysis markers and α-SMA. These findings indicate that activated PSCs play an important role in the development and progression of chronic pancreatitis into pancreatic cancer by regulating and promoting aerobic glycolysis. Our research provides a new theoretical basis for further understanding the mechanism of CP malignancy and the selection of targets for reversing CP malignancy.

scholarly journals Activated Pancreatic Stellate Cells Enhance the Warburg Effect to Cause the Malignant Development in Chronic Pancreatitis

2021 ◽  
Vol 11 ◽  
Author(s):  
Ye Tao ◽  
Feng Shao ◽  
Ming Cai ◽  
Zhen Liu ◽  
Yao Peng ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Chronic Pancreatitis ◽  
Epithelial Cells ◽  
Pancreatic Duct ◽  
Cancer Cells ◽  
Stellate Cells ◽  
Monocarboxylate Transporter ◽  
Pancreatic Stellate Cells ◽  
Glucose Transporter 1 ◽  
Pancreatic Cancer Cells

Chronic pancreatitis (CP) is a precancerous condition associated with pancreatic ductal adenocarcinoma (PDAC), but its evolutionary mechanism is unclear. Pancreatic stellate cells (PSCs) are closely related to the occurrence and development of CP and PDAC, but it is not clear whether PSCs play a key role in this “inflammation-cancer transition”. Our research found that co-culture with activated PSCs promoted the proliferation, migration and invasion of normal pancreatic duct epithelial cells and pancreatic cancer cells. At the same time, activated PSCs had a significant effect on the expression of the glycolysis markers (pyruvate kinase M2, lactate dehydrogenase A, glucose transporter 1, hexokinase-II and monocarboxylate transporter 4; PKM2, LDHA, GLUT1, HK2 and MCT4) in normal pancreatic duct epithelial cells and pancreatic cancer cells and increased lactic acid production and glucose consumption in these two cells. In vivo experiments showed that the expression of the glycolysis markers in pancreatic duct epithelial cells and the marker protein (α-SMA) of activated PSCs in the pancreatic duct peripancreatic interstitium were higher in pancreatic cancer tissues and chronic pancreatitis tissues than in normal pancreatic tissues in both animals and humans. In addition, analysis of human tissue specimens showed that there is a correlation between the expression of glycolysis markers and α-SMA. These findings indicate that activated PSCs play an important role in the development and progression of chronic pancreatitis into pancreatic cancer by regulating and promoting aerobic glycolysis. Our research provides a new theoretical basis for further understanding the mechanism of CP malignancy and the selection of targets for reversing CP malignancy.

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