scholarly journals Presymptomatic Transmission of Severe Acute Respiratory Syndrome Coronavirus 2 Among Residents and Staff at a Skilled Nursing Facility: Results of Real-time Polymerase Chain Reaction and Serologic Testing

2020 ◽  
Author(s):  
Scott A Goldberg ◽  
Jochen Lennerz ◽  
Michael Klompas ◽  
Eden Mark ◽  
Virginia M Pierce ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Severe Acute Respiratory Syndrome ◽  
Real Time ◽  
Skilled Nursing Facility ◽  
Chain Reaction ◽  
Nursing Facility ◽  
Skilled Nursing ◽  
Routine Testing ◽  
Serologic Testing ◽  
Polymerase Chain

Abstract High rates of asymptomatic coronavirus disease 2019 infection suggest benefits to routine testing in congregate care settings. Screening was undertaken in a single nursing facility without a known case of coronavirus disease 2019, demonstrating an 85% prevalence among residents and 37% among staff. Serology was not helpful in identifying infections.

scholarly journals Using Serologic Testing to Assess the Effectiveness of Outbreak Control Efforts, Serial Polymerase Chain Reaction Testing, and Cohorting of Positive Severe Acute Respiratory Syndrome Coronavirus 2 Patients in a Skilled Nursing Facility

2020 ◽  
Author(s):  
Amy V Dora ◽  
Alexander Winnett ◽  
Jennifer A Fulcher ◽  
Linda Sohn ◽  
Feliza Calub ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Nursing Home ◽  
Severe Acute Respiratory Syndrome ◽  
Skilled Nursing Facility ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Nursing Facility ◽  
Skilled Nursing ◽  
Polymerase Chain Reaction Testing ◽  
Polymerase Chain

Abstract We characterized serology following a nursing home outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) where residents were serially tested by reverse-transcription polymerase chain reaction (RT-PCR) and positive residents were cohorted. When tested 46–76 days later, 24 of 26 RT-PCR–positive residents were seropositive; none of the 124 RT-PCR–negative residents had confirmed seropositivity, supporting serial SARS-CoV-2 RT-PCR testing and cohorting in nursing homes.

scholarly journals Multiplex Real-Time Reverse-Transcription Polymerase Chain Reaction Assays for Diagnostic Testing of Severe Acute Respiratory Syndrome Coronavirus 2 and Seasonal Influenza Viruses: A Challenge of the Phase 3 Pandemic Setting

2020 ◽  
Author(s):  
Fabiola Mancini ◽  
Fabrizio Barbanti ◽  
Maria Scaturro ◽  
Stefano Fontana ◽  
Angela Di Martino ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Severe Acute Respiratory Syndrome ◽  
Real Time ◽  
Reverse Transcription ◽  
Seasonal Influenza ◽  
Influenza Viruses ◽  
Activity Period ◽  
Clinical Samples ◽  
Chain Reaction ◽  
Polymerase Chain

Abstract Background Pandemic coronavirus disease 2019 (COVID-19) disease represents a challenge for healthcare structures. The molecular confirmation of samples from infected individuals is crucial and therefore guides public health decision making. Clusters and possibly increased diffuse transmission could occur in the context of the next influenza season. For this reason, a diagnostic test able to discriminate severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from influenza viruses is urgently needed. Methods A multiplex real-time reverse-transcription polymerase chain reaction (PCR) assay was assessed using 1 laboratory protocol with different real-time PCR instruments. Overall, 1000 clinical samples (600 from samples SARS-CoV-2–infected patients, 200 samples from influenza-infected patients, and 200 negative samples) were analyzed. Results The assay developed was able to detect and discriminate each virus target and to intercept coinfections. The limit of quantification of each assay ranged between 5 and 10 genomic copy numbers, with a cutoff value of 37.7 and 37.8 for influenza and SARS-CoV-2 viruses, respectively. Only 2 influenza coinfections were detected in COVID-19 samples. Conclusions This study suggests that multiplex assay is a rapid, valid, and accurate method for the detection of SARS-CoV-2 and influenza viruses in clinical samples. The test may be an important diagnostic tool for both diagnostic and surveillance purposes during the seasonal influenza activity period.

scholarly journals Real-Time Polymerase Chain Reaction for detecting SARS-COV-2 in Indonesia: Are the results reliable?

2020 ◽  
Vol 39 (2) ◽  
pp. 71
Author(s):  
Monica Dwi Hartanti
Keyword(s):  
Polymerase Chain Reaction ◽  
Severe Acute Respiratory Syndrome ◽  
Real Time ◽  
Real Time Pcr ◽  
Reliable Method ◽  
Chain Reaction ◽  
Indonesian Government ◽  
Diagnostic Management ◽  
Polymerase Chain

<p>Since it was first reported in Wuhan, China in December 2019, coronavirus disease 2019 (COVID-19) has been considered as a pandemic. It is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a member of the Coronavirus family. In Indonesia, this disease first detected in the middle of March 2020 and it was spreading all over Indonesia. The Indonesian Government has implemented several strategies in order to detain the spreading of COVID-19, including improving diagnostic management. Until now, the most reliable method of detecting COVID-19 is real time-PCR.</p>

scholarly journals Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection in Febrile Infants Without Respiratory Distress

2020 ◽  
Vol 71 (16) ◽  
pp. 2243-2245 ◽  
Author(s):  
Michal Paret ◽  
Jennifer Lighter ◽  
Rebecca Pellett Madan ◽  
Vanessa N Raabe ◽  
Gail F Shust ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Severe Acute Respiratory Syndrome ◽  
Respiratory Distress ◽  
Real Time ◽  
Reverse Transcription ◽  
Bacterial Infections ◽  
Chain Reaction ◽  
Polymerase Chain

Abstract We report 2 cases of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection (COVID-19) in infants presenting with fever in the absence of respiratory distress who required hospitalization for evaluation of possible invasive bacterial infections. The diagnoses resulted from routine isolation and real-time reverse-transcription polymerase chain reaction–based testing for SARS-CoV-2 for febrile infants in an outbreak setting.

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