Fluorometric Determination of Zinc in Biologic Fluids

1968 ◽  
Vol 14 (1) ◽  
pp. 6-11 ◽  
Author(s):  
D Mahanand ◽  
J C Houck

Abstract The 8-quinolinol complexes of zinc at pH 8.0 (in universal buffer) are stabilized by gum arabic and demonstrate a marked fluorescence at 517 mµ when excited at 375 mµ. Under these conditions only zinc—not magnesium or calcium at physiologic concentrations—can be determined in plasma and urine. Adult plasma has 94 ± 7 µg. zinc per 100 ml. while the plasma of children contains 108 ± 15g./100 ml.

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
A Copra-Janicijevic ◽  
E Sofic ◽  
L Klepo ◽  
A Topcagic ◽  
I Tahirovic ◽  
...  

1964 ◽  
Vol 47 (2) ◽  
pp. 203-208
Author(s):  
Stanley E Katz ◽  
Joseph Spock

Abstract The fluorometric procedure for chlortetracycline in mixed feeds is based upon the degradation of chlortetracycline in alkaline solution to isochlortetracycline. The fluorescence of the isochlortetracycline is directly proportional to the concentration of chlortetracycline present prior to alkaline degradation. Chlortetracycline is extracted from mixed feeds with an acidmethanol solvent system, separated from the feed extract by adsorption on a Dowex 50 ion exchange resin, and eluted from the resin by ammonium hydroxide after conversion to isochlortetracycline. The isochlortetracycline is measured fluorometrically. Recoveries from known feeds are generally greater than 90% and agreements with microbiological assays are very close.


1980 ◽  
Vol 69 (3) ◽  
pp. 275-279 ◽  
Author(s):  
J.-S.K. Shim ◽  
J.W. Tolan ◽  
D.W. Fink

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