Application of a Continuous Spectrophotometric Assay for 5'Nucleotidase Activity in Normal Subjects and Patients with Liver and Bone Disease

1969 ◽  
Vol 15 (10) ◽  
pp. 931-939 ◽  
Author(s):  
Alan Belfield ◽  
David M Goldberg
Keyword(s):  
Alkaline Phosphatase ◽  
Bone Disease ◽  
Serum Alkaline Phosphatase ◽  
Hepatic Metastases ◽  
Normal Subjects ◽  
Enzyme Concentration ◽  
Nucleotidase Activity ◽  
Kinetic Assay ◽  
Serum Alkaline Phosphatase Activity ◽  
Parenchymal Liver

Abstract Serum 5'nucleotidase activity has been measured by a coupled kinetic assay in which adenosine formed by hydrolysis of adenosine 5'-monophosphate in the presence of 150-fold excess of β-glycerophosphate is converted to inosine by adenosine deaminase, with a consequent decrease in absorbance at 265 nm. The method gives activity in proportion to enzyme concentration so long as the rate of decrease of absorbance at 265 nm does not exceed 0.025/min, and not more than 30% of the substrate is consumed. The normal range established in 517 healthy adults was 0-15 mU/ml. A significant correlation between enzyme activity and age was found in females but not in males. Raised levels of 5'nucleotidase activity were found in 92% of patients with obstructive jaundice, 70% of patients with parenchymal liver disease, 81% of patients with hepatic metastases, and 11% of patients with bone disease. The estimation is useful in aiding the elucidation of raised serum alkaline phosphatase activity, and is of value as a liver function test, but is not as frequently increased as alkaline phosphatase in all classes of hepatobiliary disease.

Serum Gamma-Glutamyl Transpeptidase Activity as an Indicator of Disease of Liver, Pancreas, or Bone

1972 ◽  
Vol 18 (4) ◽  
pp. 358-362 ◽  
Author(s):  
Gifford Lum ◽  
S Raymond Gambino
Keyword(s):  
Alkaline Phosphatase ◽  
Liver Disease ◽  
Viral Hepatitis ◽  
Bone Disease ◽  
Leucine Aminopeptidase ◽  
Serum Alkaline Phosphatase ◽  
Metastatic Carcinoma ◽  
Gamma Glutamyl Transpeptidase ◽  
Serum Alkaline Phosphatase Activity ◽  
Glutamyl Transpeptidase

Abstract Serum γ-glutamyl transpeptidase (GGT), leucine aminopeptidase, alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase activities were assayed in controls and in patients with liver, pancreatic, or bone disease. GGT activity was above normal in all forms of liver disease studied (viral hepatitis, cirrhosis, cholecystitis, metastatic carcinoma to liver, pancreatic carcinoma, liver granuloma, and acute pancreatitis). GGT more sensitively indicated hepatic disease than did alkaline phosphatase, much more so than did leucine aminopeptidase. GGT was disproportionately more active in relation to the transaminases in cases of intraor extrahepatic biliary obstruction; the reverse was true in cases of viral hepatitis. GGT activity was normal in children, adolescents, and pregnant women, and in cases of bone disease and renal failure. Kinetic measurement of GGT activity offers a simple, sensitive, and direct means for distinguishing whether bone or liver is the source of increased serum alkaline phosphatase activity. Activity was highest in obstructive liver disease.

Serum alkaline phosphatase activity is regulated by a chromosomal region containing the alkaline phosphatase 2 gene (Akp2) in C57BL/6J and DBA/2J mice

2005 ◽  
Vol 23 (3) ◽  
pp. 295-303 ◽  
Author(s):  
Jennifer E. Foreman ◽  
David A. Blizard ◽  
Glenn Gerhard ◽  
Holly A. Mack ◽  
Dean H. Lang ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Chromosomal Region ◽  
Serum Alkaline Phosphatase ◽  
Inbred Strains ◽  
Lod Score ◽  
Serum Alkaline Phosphatase Activity ◽  
Single Nucleotide ◽  
Significance Level ◽  
Hepatic Expression ◽  
Trait Locus

Quantitative trait locus (QTL) analyses were conducted to identify chromosomal regions that contribute to variability in serum alkaline phosphatase (AP) enzyme activity in mice derived from the C57BL/6J (B6) and DBA/2J (D2) inbred strains. Serum AP was measured in 400 B6D2 F2 mice at 5 mo and 400 B6D2 F2 mice at 15 mo of age that were genotyped at 96 microsatellite markers, and in 19 BXD recombinant inbred (RI) strains at 5 mo of age. A QTL on the distal end of chromosome 4 was present in all sex- and age-specific analyses with a peak logarithm of odds (LOD) score of 20.36 at 58.51 cM. The Akp2 gene, which encodes the major serum AP isozyme, falls within this QTL region at 70.2 cM where the LOD score reached 13.2 (LOD significance level set at 4.3). Serum AP activity was directly related to the number of D2 alleles of a single nucleotide polymorphism in the 5′-flanking region of the Akp2 gene, although no strain-related differences in hepatic expression of Akp2 RNA were found. A variety of sequence polymorphisms in this chromosomal region could be responsible for the differences in serum AP activity; the Akp2 gene, however, with several known amino acid substitutions between protein sequences of the B6 and D2 strains, is a leading candidate.

Rickets Associated With Long-Term Anticonvulsant Therapy in a Pediatric Outpatient Population

PEDIATRICS ◽  
1975 ◽  
Vol 56 (1) ◽  
pp. 52-57 ◽  
Author(s):  
Carl J. Crosley ◽  
Claire Chee ◽  
Peter H. Berman
Keyword(s):  
Alkaline Phosphatase ◽  
Alkaline Phosphatase Activity ◽  
Serum Alkaline Phosphatase ◽  
Pediatric Population ◽  
Anticonvulsant Therapy ◽  
Control Population ◽  
Serum Alkaline Phosphatase Activity ◽  
Calcium And Phosphorus ◽  
And Control

Over a 12-month period, an ambulatory pediatric population receiving long-term anticonvulsants was surveyed for the presence of biochemical and radiologic rickets. There were 74 treated children and 95 matched controls. Elevations of serum alkaline phosphatase activity occurred in 31 of the 74 (42%) treated children (23 of 47 children between 2 and 10 years and 8 of 21 children between 10 and 16 years). This frequency of abnormal values was significantly greater than that which occurred in our control population. Calcium and phosphorus abnormalities were minimal in both treated and control populations. Radiologic rickets occurred in 6 of the 74 (8%) of the treated children and in none of the control population. Neither the severity of the rickets nor the degree of hyperalkaline phosphatasemia were correlated with age of the patient, duration, and/or dose of anticonvulsant therapy.

Alkaline phosphatase activity in the plasma of children and adolescents.

1977 ◽  
Vol 23 (3) ◽  
pp. 469-472 ◽  
Author(s):  
G A Fleisher ◽  
E S Eickelberg ◽  
L R Elveback
Keyword(s):  
Alkaline Phosphatase ◽  
Public Schools ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Serum Alkaline Phosphatase ◽  
Intraclass Correlation ◽  
Serum Alkaline Phosphatase Activity ◽  
Upper Limits ◽  
Prepubertal Girls

Abstract We determined plasma (serum alkaline phosphatase activity in 854 healthy students of the Rochester, Minnesota, public schools. Prepubertal girls had somewhat greater upper limits than did boys, and there was a low trend of increasing activity in both sexes. At the beginning of adolescence increasing activities were observed, which peaked at ages 11 to 12 years in girls and at ages 13 to 14 in boys. Adult values were not reached until six to eight years later. In 180 pairs of siblings, a significant intraclass correlation was noted. A possible role of alkaline phosphatase in the regulation of protein synthesis is suggested.

A Continuous Spectrophotometric Method for Measuring the Activity of Serum Alkaline Phosphatase

1966 ◽  
Vol 12 (2) ◽  
pp. 70-89 ◽  
Author(s):  
George N Bowers ◽  
Robert B McComb
Keyword(s):  
Alkaline Phosphatase ◽  
Standard Deviation ◽  
Sample Size ◽  
Spectrophotometric Method ◽  
Serum Alkaline Phosphatase ◽  
Serum Alkaline Phosphatase Activity ◽  
Factors Affecting ◽  
Nitrophenyl Phosphate ◽  
Relative Standard ◽  
Hydrolysis Of

Abstract A continuous spectrophotometric method for measuring serum alkaline phosphatase activity is described. The effects of temperature, pH, substrate concentration, type and molarity of the buffer, sample size, cofactors, and inhibitors on the enzymatic hydrolysis of p-nitrophenyl phosphate were studied. The optimal conditions for assay of serum alkaline phosphatase at 30° were found to be 0.75 M 2-amino-2-methyl-1-propanol buffer, pH30° 10.15, 4 mmole substrate, and 100 µl. or less sample size. Studies of the factors affecting analytical precision-i.e., control of reaction temperature, of reagent manufacture, and of standardization-are discussed. The precision of this method was 2.3% (relative standard deviation) on 10 within day replicates and 5.0% on day-to-day replicates spread over a 5-week period. The range of activity for 258 apparently healthy adult blood donors was 6-110 mU./ml. (International milliunits per milliliter), with a mean of 49 and a standard deviation of 14.

Effects of various fibrates on serum alkaline phosphatase activity

2002 ◽  
Vol 165 (1) ◽  
pp. 187-188 ◽  
Author(s):  
Emanuel Ganotakis ◽  
Vasilios Tsimihodimos ◽  
Eleni Bairaktari ◽  
Evagelos Rizos ◽  
Vasilios Athyros ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Serum Alkaline Phosphatase ◽  
Serum Alkaline Phosphatase Activity
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