Emergency Gas—Liquid Chromatographic Determination of Barbiturates and Glutethimide in Serum
Keyword(s):
Abstract A method is presented for the rapid gas chromatographic determination of barbiturates and glutethimide in serum, suitable for use in clinical emergencies. The sample is prepared for gas chromatography by acidification and a single extraction with chloroform. From 70 to 100% of the various barbiturates and glutethimide are analytically recovered, with a coefficient of variation of 8 to 10% for the method. During a 10-month clinical evaluation (87 requested determinations), 34 analyses (39% of the samples submitted) were positive for barbiturates, glutethimide, or both.
1988 ◽
Vol 71
(3)
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pp. 480-484
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1991 ◽
Vol 74
(5)
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pp. 780-784
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1990 ◽
Vol 73
(3)
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pp. 368-373
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