Improved method for measurement of inorganic phosphate in serum with a centrifugal analyzer.

1976 ◽  
Vol 22 (2) ◽  
pp. 257-260 ◽  
Author(s):  
P W Wentz ◽  
J Savory ◽  
R E Cross
Keyword(s):  
Plasma Cell ◽  
Data Reduction ◽  
Continuous Flow ◽  
Inorganic Phosphate ◽  
High Speed ◽  
Improved Method ◽  
Plasma Cell Dyscrasias

Abstract A direct mehtod [Clin. Chim. Acta 46, 113 (1973)] for determination of inorganic phosphate in serum was adapted for use with a centrifugal analyzer. Contamination is minimized and analysis rate maximized by doing the reaction in the reagent wells of the transfer disc and by utilizing the high-speed spectrophotometric and data-reduction capabilities of the centrifugal analyzer. Hemolysis, icterus, and moderate lipemia cause no interference. Grossly lipemic sera and sera from patients with plasma cell dyscrasias can be analyzed by incorporating appropriate blanking and dilution techniques. The method exhibits excellent sensitivity and precision and results correlate well with those from a continuous-flow procedure.

scholarly journals Continuous Flow Techniques for the Determination of Serum Calcium and the Simultaneous Determination of Calcium and Inorganic Phosphate in Serum and Urine

1974 ◽  
Vol 11 (1-6) ◽  
pp. 81-85
Author(s):  
W. G. Brydon
Keyword(s):  
Simultaneous Determination ◽  
Serum Calcium ◽  
Continuous Flow ◽  
Inorganic Phosphate ◽  
Methyl Green ◽  
Fluorometric Method ◽  
Precision Data ◽  
Accuracy And Precision ◽  
Serum And Urine

The fluorometric method of Fingerhut et al. (1969) for the determination of serum calcium has been modified to approximately treble the sensitivity. Using only 120 μl of sample a method has been developed for the simultaneous determination of calcium and inorganic phosphate in serum and urine, the phosphate being measured by complexing phosphomolybdate with methyl green, a method developed by Van Belle (1970). Only a single dialysis stage is required, and reagents and sample can be delivered using an AutoAnalyzer Pump I. The techniques correlate well with other methods. Accuracy and precision data are presented.

scholarly journals Combined method of PAP immunocytochemistry and autoradiography: application to cell kinetic study in plasma cell dyscrasias.

1987 ◽  
Vol 35 (10) ◽  
pp. 1157-1160
Author(s):  
T Kanoh ◽  
T Ohnaka ◽  
H Uchino
Keyword(s):  
Plasma Cell ◽  
Plasma Cells ◽  
Cell Labeling ◽  
Combined Method ◽  
Cell Kinetic ◽  
Diagnosis And Prognosis ◽  
Plasma Cell Dyscrasias ◽  
Pap Method ◽  
Combined Technique

The plasma cell labeling index (LI), in spite of being a reliable indicator for diagnosis and prognosis of multiple myeloma, has been measured in a limited number of laboratories because of technical difficulties. We have developed a new combined technique, using the peroxidase-antiperoxidase (PAP) method and autoradiography, which has several advantages over previously described methods. The primary advantages of our method in the determination of lymphoid-plasma cell LI% are: (a) no damage to slides during storage of more than 1 year; (b) an exact LI measurement in each morphological variety of pleomorphic immunoglobulin-containing cells; (c) no problem in differentiation of lymphoid plasma cells from early red cell precursors; and (d) a separate LI measurement for those lymphoid-plasma cells composed chiefly, if not exclusively, of monoclonal or neoplastic cells. Because of these advantages, this accurate and less difficult technique will facilitate performance of lymphoid plasma cell LI in a number of laboratories.

Automated determination of urinary Na+, K+, chloride, inorganic phosphate, urea, and creatinine without sample dilution, with the "RA-XT".

1989 ◽  
Vol 35 (3) ◽  
pp. 481-483
Author(s):  
P D Fesus ◽  
M Pressac ◽  
F Braconnier ◽  
P Aymard
Keyword(s):  
Continuous Flow ◽  
Inorganic Phosphate ◽  
Room Temperature ◽  
Flow Analysis ◽  
Continuous Flow Analysis ◽  
Sample Dilution ◽  
Automated Determination

Abstract We describe how concentrations of chloride, urea, inorganic phosphate, and creatinine in urine can be measured directly, without manual sample dilution, in a discrete analyzer (the Technicon "RA-XT"). These methods were accurate for concentrations of chloride up to 280 mmol/L, urea up to 500 mmol/L, inorganic phosphate up to 50 mmol/L, and creatinine up to 30 mmol/L. CVs are less than 3% nd results correlate well with those obtained by continuous-flow analysis (SMA-II). All these reagents are stable at room temperature for three weeks. Analyses are easy to perform and infrequent calibration is required.

Continuous-flow assay with immobilized enzymes for determining of inorganic phosphate in serum

1995 ◽  
Vol 41 (1) ◽  
pp. 99-102 ◽  
Author(s):  
M D Luque de Castro ◽  
R Quiles ◽  
J M Fernández-Romero ◽  
E Fernández
Keyword(s):  
Flow Injection Analysis ◽  
Continuous Flow ◽  
Inorganic Phosphate ◽  
Enzymatic Reaction ◽  
Immobilized Enzymes ◽  
Excellent Correlation ◽  
Serum Samples ◽  
Automated Method ◽  
Method R

Abstract An automated method for the determination of inorganic phosphate based on flow-injection analysis and on the use of immobilized enzymes is reported. The method features a linear range between 0.1 and 20 mumol/L with a CV < 2.1% and 3.4% for the within-run and between-run studies, respectively, and a sampling throughput of 40 h-1. The sensitivity of the method makes a 1:250 dilution of the serum samples feasible, thus making undetectable the interferences from analytes commonly present in serum. The method shows an excellent correlation with conventional automated analyzers based on the same enzymatic reaction (Hitachi, r = 0.988) but with the catalyst in solution, and with the Kodak Ektachem method (r = 0.974) based on the use of dry reagents and formation of the phosphomolybdo heteropolyacid.

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