scholarly journals Improved method for the automatic determination of serum inorganic phosphate.

1966 ◽  
Vol 19 (4) ◽  
pp. 397-399 ◽  
Author(s):  
D S Young
Keyword(s):  
Inorganic Phosphate ◽  
Improved Method ◽  
Automatic Determination ◽  
Serum Inorganic Phosphate

Improved method for measurement of inorganic phosphate in serum with a centrifugal analyzer.

1976 ◽  
Vol 22 (2) ◽  
pp. 257-260 ◽  
Author(s):  
P W Wentz ◽  
J Savory ◽  
R E Cross
Keyword(s):  
Plasma Cell ◽  
Data Reduction ◽  
Continuous Flow ◽  
Inorganic Phosphate ◽  
High Speed ◽  
Improved Method ◽  
Plasma Cell Dyscrasias

Abstract A direct mehtod [Clin. Chim. Acta 46, 113 (1973)] for determination of inorganic phosphate in serum was adapted for use with a centrifugal analyzer. Contamination is minimized and analysis rate maximized by doing the reaction in the reagent wells of the transfer disc and by utilizing the high-speed spectrophotometric and data-reduction capabilities of the centrifugal analyzer. Hemolysis, icterus, and moderate lipemia cause no interference. Grossly lipemic sera and sera from patients with plasma cell dyscrasias can be analyzed by incorporating appropriate blanking and dilution techniques. The method exhibits excellent sensitivity and precision and results correlate well with those from a continuous-flow procedure.

Continuous-flow determination of serum inorganic phosphate with a single reagent--the vanadomolybdate method re-evaluated.

1977 ◽  
Vol 23 (7) ◽  
pp. 1275-1280 ◽  
Author(s):  
H Baadenhuijsen ◽  
H E Seuren-Jacobs ◽  
A P Jansen
Keyword(s):  
Acid Medium ◽  
Inorganic Phosphate ◽  
Room Temperature ◽  
Visible Range ◽  
Color Development ◽  
Optimum Reaction ◽  
The Stability ◽  
Serum Inorganic Phosphate ◽  
Tungsten Lamp

Abstract The merits of the vanadomolybdate method in the determination of inorganic phosphate are highly underestimated with regard to the simplicity of the method and the stability of both the reagents and the color that is formed. The absorption curve of the phosphovanadomolybdate complex, with its maximum at 335 nm, extends into the visible range of the spectrum. This permits measurements with inexpensive tungsten-lamp colorimeters. On-stream dialysis is best done in a nitric acid medium, 0.15 mol/liter. Paralleled by the change in the H2PO4-/H3PO4 ratio, appreciable protein binding and poor dialysis efficiency are seen at lower acid concentrations (pH greater than 1.0). Optimum reaction-mixture concentrations of vanadium and molybdenum appeared to be respectively 0.2 and 5 mmol/liter up to 3 mmol of phosphate per liter, in a final acid medium of 0.2 mol/liter, concentrations considerably lower than those used in some studies published earlier. Color development with the stable combined reagent is complete after only 20 s at room temperature and the color is stable for at least 2 h. Figures on precision and accuracy demonstrate the reliability of the method.

scholarly journals Assay of Serum Inorganic Phosphate without Deproteinisation: Automated and Manual Micromethods

1974 ◽  
Vol 11 (1-6) ◽  
pp. 234-237 ◽  
Author(s):  
R. Lawrence
Keyword(s):  
Inorganic Phosphate ◽  
Sodium Lauryl Sulphate ◽  
Good Precision ◽  
Molybdenum Blue ◽  
Blue Reaction ◽  
Serum Inorganic Phosphate

Several current techniques for the determination of serum inorganic phosphate in the presence of proteins are shown to have important disadvantages. An improved manual micromethod is described in which proteins are kept in solution by the use of sodium lauryl sulphate and the phosphate determined by the molybdenum blue reaction. The procedure has been adapted for the Technicon AutoAnalyzer and the Vickers D-300 and M-300, and has been shown to give very good precision in routine use.

Error introduced by specimen handling before determination of inorganic phosphate concentrations in plasma and serum.

1976 ◽  
Vol 22 (11) ◽  
pp. 1909-1911 ◽  
Author(s):  
J E Carothers ◽  
N M Kurtz ◽  
J Lemann
Keyword(s):  
Coefficient Of Variation ◽  
Inorganic Phosphate ◽  
Room Temperature ◽  
Processing Technique ◽  
Healthy Adults ◽  
Blood Sampling ◽  
Specimen Handling ◽  
Evacuated Tubes ◽  
Serum Inorganic Phosphate

Abstract Values for serum inorganic phosphate (Pi) concentrations in groups of healthy adults vary widely, the coefficient of variation ranging from 10 to 15%. We undertook to determine in 23 healthy adults whether part of this variation could be accounted for by (a) drawing blood in syringes vs. evacuated tubes (b) the time between blood sampling and separation of serum or plasma, and (c) the prevention of clotting. Values were unaffected by a, decreased significantly with time at room temperature between blood sampling and separation of cells in both serum and plasma, and were significantly lower in plasma than in serum. The group coefficient of variation for Pi averaged 13% and was uninfluenced by the blood-processing technique.

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