Improved continuous-flow method for detemination of total serum hexosamines.
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Abstract We describe an automated determination of serum hexosamine by the Elson-Morgan reaction, together with reagent modifications that minimiz interference from amino acids and sugars present in acid hydrolysates of sera. We used a novel 15-min autoclave procedure for the acid hydrolysis of sera before analysis to facilitate the detemination as compared to the 4-h hydrolysis used in the conventional manual method. Results correlated well (r = 0.906) with those obtained by the corresponding manual method.
1975 ◽
Vol 35
(3)
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pp. 267-273
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1975 ◽
Vol 62
(3)
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pp. 435-438
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2007 ◽
Vol 41
(3)
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pp. 243-248
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1983 ◽
Vol 152
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pp. 295-299
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1992 ◽
Vol 258
(2)
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pp. 253-258
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