Mass-fragmentographic determination of catecholamine metabolites in amniotic fluid and its possible clinical usefulness.

1978 ◽  
Vol 24 (11) ◽  
pp. 1899-1902 ◽  
Author(s):  
F A Muskiet ◽  
H J Jeuring ◽  
G T Nagel ◽  
H W de Bruyn ◽  
B G Wolthers
Keyword(s):  
Amniotic Fluid ◽  
Gestational Age ◽  
Phenylacetic Acid ◽  
Mass Fragmentography ◽  
Absolute Concentration ◽  
Creatinine Concentration ◽  
Internal Standards ◽  
Catecholamine Metabolites ◽  
Congenital Neuroblastoma

Abstract We describe the determination of 3-methoxy-4-hydroxy-phenylacetic acid, 3-methoxy-4-hydroxyphenylamandelic acid, and 3-methoxy-4-hydroxyphenylethylene glycol in amniotic fluid by means of mass fragmentography, with use of deuterated internal standards. The results expressed in terms of absolute concentration and creatinine concentration, are given as a function of gestational age. In the 15th to 17th week, concentrations in amniotic fluid are a reflection of those in the mother's serum, whereas in the 32nd to 40th week, these concentrations, expressed in terms of creatinine, are similar to those found in the urine of newborns. We discuss the possible usefulness of the determination of catecholamine metabolites in amniotic fluid in the diagnosis of congenital neuroblastoma, maternal pheochromocytoma, and underdevelopment.

Determination of 3-methoxy-4-hydroxyphenylpyruvic acid, 3,4-dihydroxyphenylethylene glycol, and 3,4-dihydroxyphenylmandelic acid in urine by mass fragmentography, with use of deuterium-labeled internal standards.

1978 ◽  
Vol 24 (11) ◽  
pp. 2001-2008 ◽  
Author(s):  
F A Muskiet ◽  
D C Fremouw-Ottevangers ◽  
G T Nagel ◽  
B G Wolthers ◽  
J A de Vries
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Mass Fragmentography ◽  
Decarboxylase Inhibitor ◽  
Purification Method ◽  
Internal Standards ◽  
Chromatography Mass Spectrometry ◽  
Catecholamine Metabolites

Abstract We report the determination of 3-methoxy-4-hydroxyphenylpyruvic acid, 3,4-dihydroxyphenylmandelic acid, and 3,4-dihydroxyphenylethylene glycol in urine, by use of gas chromatography/mass spectrometry in combination with a simple purification method and deuterium-labeled internal standards. Normal excretion values in terms of creatinine, expressed as a function of age, are given, together with results obtained for patients with neuroblastoma, pheochromocytoma, or parkinsonism treated with L-DOPA + peripheral decarboxylase inhibitor, and for a patient receiving dopamine. We were unable to identify 3, 4-dihydroxyphenyllactic acid in urine. The results obtained and their relation to other catecholamine metabolites and catecholamine-precursor metabolites in urine are discussed.

Prediction of Fetal Lung and Kidney Maturity by Determination of Amniotic Fluid Lecithin: Sphingomyelin Ratio and Creatinine Concentration

1974 ◽  
Vol 19 (5) ◽  
pp. 229-232 ◽  
Author(s):  
R. D. Paton ◽  
R. W. Logan ◽  
J. MacVicar
Keyword(s):  
Amniotic Fluid ◽  
Gestational Age ◽  
Fetal Lung ◽  
Creatinine Concentration ◽  
Fetal Lung Maturity ◽  
Wide Range ◽  
Immature Lung ◽  
Lung Maturity ◽  
Lung And Kidney

The ratio of lecithin to sphingomyelin (L/S) in amniotic fluid was assessed in 209 patients on 275 occasions and confirmed to be a useful index of fetal lung maturity. Although low and high ratios were found mainly at early and late gestational ages respectively, lung maturity (L/S ratio ≥ 3:1) occurred at a wide range of ages, from 28 to 40 weeks. The L/S ratio was, therefore, not found to be a useful index of gestational age in individual cases. In 174 cases, in addition to determining the L/S ratio, the concentration of creatinine in the liquor was estimated as an index of renal maturity. (Creatinine ≥ 1.6 mg./100 ml. ≡ renal maturity). In 80 per cent of cases, lung and kidney were judged to be both mature or both immature. In the remainder, combinations of mature kidney/immature lung or immature kidney/mature lung existed. Liquor creatinine estimation remains useful as a guide to gestational age and as an adjunct to lung maturity studies.

Determination of 4-O-methylated catecholamine metabolites in urine by mass fragmentography.

1979 ◽  
Vol 25 (10) ◽  
pp. 1708-1713 ◽  
Author(s):  
F A Muskiet ◽  
D C Fremouw-Ottevangers ◽  
G T Nagel ◽  
B G Wolthers
Keyword(s):  
Urinary Excretion ◽  
Intravenous Administration ◽  
Normal Values ◽  
Mass Fragmentography ◽  
Internal Standards ◽  
Hydroxyphenylacetic Acid ◽  
Catecholamine Metabolites ◽  
The Mean

Abstract We describe the determination of the 4-O-methylated catecholamine metabolites 4-methoxy-3-hydroxyphenylacetic acid (iso-HVA), 4-methoxy-3-hydroxyphenylmandelic acid (iso-VMA) and 4-methoxy-3-hydroxyphenylethylene glycol (iso-MOPEG) in urine with use of mass fragmentography and deuterated internal standards. Normal values, expressed in terms of creatinine, are given as a function of age. Urinary excretion values during neuroblastoma, pheochromocytoma, and parkinsonism treated with L-DOPA, and during the intravenous administration of dopamine are discussed. For normal persons, the mean percentages (+/- SD) of 4-O-methylated metabolites relative to their 3-O-methylated analogs were 6.29 +/- 2.79% (iso-HVA/HVA), 0.70 +/- 0.57% (iso-VMA/VMA), and 0.77 +/- 0.53% (iso-MOPEG/MOPEG). We observed no significant changes in these parameters for the patients studied and conclude that the determination of 4-O-methylated catecholamine metabolites for these patients does not add information to the results obtained from their quantitatively more important 3-O-methylated analogs.

Determination of catecholamines and their 3-O-methylated metabolites in urine by mass fragmentography with use of deuterated internal standards.

1979 ◽  
Vol 25 (3) ◽  
pp. 453-460 ◽  
Author(s):  
F A Muskiet ◽  
C G Thomasson ◽  
A M Gerding ◽  
D C Fremouw-Ottevangers ◽  
G T Nagel ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Mass Fragmentography ◽  
Decarboxylase Inhibitor ◽  
Internal Standards ◽  
Catecholamine Turnover ◽  
Catecholamine Metabolites ◽  
Normal Adults

Abstract We report the determination of catecholamines (dopamine, norepinephrine, and epinephrine) and their 3-O-methylated metabolites (3-methoxytyramine, normetanephrine, and metanephrine) in urine by gas chromatography/mass spectrometry, with use of stable isotopically labeled internal standards. Normal excretion values in terms of creatinine, expressed as a function of age, are given, together with results obtained for patients with neuroblastoma, ganglioneuroblastoma, pheochromocytoma, parkinsonism treated with L-DOPA plus peripheral decarboxylase inhibitor, and a patient with cardiogenic shock treated with dopamine. The results obtained for normal adults are discussed in relation to other catecholamine metabolites and mean overall catecholamine turnover.

Determination of nanogram amounts of catecholamine metabolites in amniotic fluid

1982 ◽  
Vol 125 (3) ◽  
pp. 291-297 ◽  
Author(s):  
Laura M. Dziedzic ◽  
Stanley W. Dziedzic ◽  
Sandra Cerqueira ◽  
Stanley E. Gitlow
Keyword(s):  
Amniotic Fluid ◽  
Catecholamine Metabolites

Methods for extracting phospholipids from human amniotic fluid compared.

1984 ◽  
Vol 30 (2) ◽  
pp. 271-274 ◽  
Author(s):  
C G Duck-Chong ◽  
G J Baker ◽  
S R Murdoch ◽  
R M Price
Keyword(s):  
Amniotic Fluid ◽  
Extraction Method ◽  
Phospholipid Composition ◽  
Fetal Lung ◽  
Absolute Concentration ◽  
Human Amniotic Fluid ◽  
Fetal Lung Maturity ◽  
Lung Maturity

Abstract Phospholipids were extracted from human amniotic fluid by various procedures, including the two most commonly applied to amniotic fluid for evaluation of fetal lung maturity. We find that the yield of phospholipid is greatly procedure dependent. This should be taken into account when one is considering the various reported methods of evaluating fetal lung maturity, because in some of them phospholipid data are expressed in terms of absolute concentration in the amniotic fluid. There were also significant differences in phospholipid composition in extracts prepared by the various procedures, but in general these were not large enough to influence evaluation of fetal lung maturity by methods in which phospholipid data are expressed in relative terms, as ratios or percentages--e.g., in the lecithin/sphingomyelin ratio and "lung profile" procedures. In the extraction method originally recommended for determination of the lecithin/sphingomyelin ratio, both the yield and composition of phospholipid depend on the extent of mixing.

Determination of some L-3,4-dihydroxyphenylalanine and dopamine metabolites in urine by means of mass fragmentography.

1978 ◽  
Vol 24 (1) ◽  
pp. 122-127 ◽  
Author(s):  
F A Muskiet ◽  
D C Fremouw-Ottevangers ◽  
J van der Meulen ◽  
B G Wolthers ◽  
J A de Vries
Keyword(s):  
Mass Fragmentography ◽  
Internal Standards ◽  
Neurogenic Tumors ◽  
Dopamine Metabolites ◽  
Dihydroxyphenylacetic Acid ◽  
Use Of Time

Abstract We describe a mass-fragmentographic method for determination in urine of the following metabolites of L-3,4-dihydroxyphenylalanine and dopamine: vanillactic acid, 3,4-dihydroxyphenylacetic acid, 3-methoxy-4-hydroxyphenylethanol, and 3,4-dihydroxyphenylethanol. Deuterated analogs were used as internal standards. The method is fast, reproducible, sensitive, and selective, and does not require the use of time-consuming clean-up procedures. Normal excretion values in terms of creatinine, expressed as a function of age, as well as values obtained for patients with neurogenic tumors, a patient during therapy with L-3,4-dihydroxyphenylalanine, and a patient receiving dopamine are presented and discussed.

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